Zobrazeno 1 - 10
of 11
pro vyhledávání: '"John L Macomber"'
Autor:
Audrey D McConnell, Minjee Do, Tamlyn Y Neben, Vladimir Spasojevic, Josh MacLaren, Andy P Chen, Laurence Altobell, John L Macomber, Ashley D Berkebile, Robert A Horlick, Peter M Bowers, David J King
Publikováno v:
PLoS ONE, Vol 7, Iss 11, p e49458 (2012)
A method has been developed for the rapid generation of high-affinity humanized antibodies from immunized animals without the need to make conventional hybridomas. Rearranged IgH D(J) regions were amplified from the spleen and lymph tissue of mice im
Externí odkaz:
https://doaj.org/article/b9ba0a64e8aa4557b73a1c4372b71869
Autor:
John L. Macomber, Sorena Rahmanian, Robert A. Horlick, David J. King, Vladimir Spasojevic, Eric Hare, Xue Zhang, Audrey D. McConnell, Peter Bowers, Joseph C. Sheffer, Betty Chau
Publikováno v:
mAbs. 6:1274-1282
Antibody engineering to enhance thermostability may enable further application and ease of use of antibodies across a number of different areas. A modified human IgG framework has been developed through a combination of engineering approaches, which
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76a55aab5480fbcf08c5fd25da0d68d1
https://doi.org/10.4161/mabs.29680
https://doi.org/10.4161/mabs.29680
Autor:
David J. King, Robert A. Horlick, Petra Verdino, Irina P. Krapf, Geoffery L. Tomlinson, John L. Macomber, Tamlyn Y. Neben, Peter Bowers, Jennifer L. Dalton
Publikováno v:
Journal of Biological Chemistry. 288:19861-19869
A mammalian expression system has been developed that permits simultaneous cell surface display and secretion of the same protein through alternate splicing of pre-mRNA. This enables a flexible system for in vitro protein evolution in mammalian cells
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45c7e828a4e19ae445f758dcbe4232fb
https://doi.org/10.1074/jbc.m113.452482
https://doi.org/10.1074/jbc.m113.452482
Autor:
Ashley D. Berkebile, Audrey D. McConnell, Peter Bowers, Joseph C. Sheffer, John L. Macomber, David J. King, Steven S. Neben, Andy P. Chen, Robert A. Horlick, Irina P. Krapf, Vladimir Spasojevich
Publikováno v:
Protein Engineering Design and Selection. 26:151-164
Antibodies are important tools for a broad range of applications due to their high specificity and ability to recognize virtually any target molecule. However, in order to be practically useful, antibodies must be highly stable and bind their target
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7315cc105800b3843017cde32deb94dc
https://doi.org/10.1093/protein/gzs090
https://doi.org/10.1093/protein/gzs090
Autor:
Laurence Altobell, Trevin Holland, Betty F. Wu, Geoffrey L. Tomlinson, Xue Zhang, Ashley D. Berkebile, Betty Chau, Rachelle M. Toobian, Robert A. Horlick, Steven S. Neben, John L. Macomber, David J. King, Jennifer L. Dalton, Tamlyn Y. Neben, Andy P. Chen, Irina P. Krapf, Heather A. Jones, Audrey D. McConnell, William J. Boyle, Peter Bowers
Publikováno v:
Proceedings of the National Academy of Sciences. 108:20455-20460
A novel approach has been developed for the isolation and maturation of human antibodies that replicates key features of the adaptive immune system by coupling in vitro somatic hypermutation (SHM) with mammalian cell display. SHM is dependent on the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e75ec7744f8b52feb415f2e0ec09038
https://doi.org/10.1073/pnas.1114010108
https://doi.org/10.1073/pnas.1114010108
Autor:
Toby Richardson, David Lam, Carl Miller, Jay M. Short, Dan E. Robertson, Mark Cabell, Gerhard Frey, Xuqiu Tan, Walter Callen, John L. Macomber
Publikováno v:
Journal of Biological Chemistry. 277:26501-26507
High throughput screening of microbial DNA libraries was used to identify α-amylases with phenotypic characteristics compatible with large scale corn wet milling process conditions. Single and multiorganism DNA libraries originating from various env
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ebc190d796d166644851b647e2f91731
https://doi.org/10.1074/jbc.m203183200
https://doi.org/10.1074/jbc.m203183200
Autor:
Irina P. Krapf, Petra Verdino, Tamlyn Y. Neben, Betty F. Wu, Eric Hare, Audrey D. McConnell, Peter Bowers, Larry Altobell, Betty Chau, Robert A. Horlick, David J. King, John L. Macomber, Ashley D. Berkebile, Marilyn Kehry, Xue Zhang, Geoffery L. Tomlinson
Publikováno v:
Methods (San Diego, Calif.). 65(1)
Recent advances are described for the isolation and affinity maturation of antibodies that couple in vitro somatic hypermutation (SHM) with mammalian cell display, replicating key aspects of the adaptive immune system. SHM is dependent on the action
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2908290eff61b3e24b23bbe39c5e177b
https://pubmed.ncbi.nlm.nih.gov/23792919
https://pubmed.ncbi.nlm.nih.gov/23792919
Autor:
Geoffery L. Tomlinson, Audrey D. McConnell, Peter Bowers, Betty F. Wu, Jennifer L. Dalton, John L. Macomber, Betty Chau, Tamlyn Y. Neben, Robert A. Horlick, David J. King, Petra Verdino, Larry Altobell, Xue Zhang, Rachelle M. Toobian
Publikováno v:
The Journal of biological chemistry. 288(11)
A method for simultaneous humanization and affinity maturation of monoclonal antibodies has been developed using heavy chain complementarity-determining region (CDR) 3 grafting combined with somatic hypermutation in vitro. To minimize the amount of m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c6cc975031adb32c7cda60c0395d6c5
https://pubmed.ncbi.nlm.nih.gov/23355464
https://pubmed.ncbi.nlm.nih.gov/23355464
Autor:
Laurence Altobell, Robert A. Horlick, Ashley D. Berkebile, Vladimir Spasojevic, Audrey D. McConnell, Peter Bowers, Tamlyn Y. Neben, David J. King, Josh MacLaren, John L. Macomber, Andy P. Chen, Minjee Do
Publikováno v:
PLoS ONE
PLoS ONE, Vol 7, Iss 11, p e49458 (2012)
PLoS ONE, Vol 7, Iss 11, p e49458 (2012)
A method has been developed for the rapid generation of high-affinity humanized antibodies from immunized animals without the need to make conventional hybridomas. Rearranged IgH D(J) regions were amplified from the spleen and lymph tissue of mice im
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c564c395749b6f3cb91ec0b66656aef
http://europepmc.org/articles/PMC3498135
http://europepmc.org/articles/PMC3498135
Autor:
Paul Dupree, Dan E. Robertson, Brian Steer, Florence Goubet, Chris S. Lyon, Nisha Palackal, Jay M. Short, Peter Luginbuhl, Geoffrey P. Hazlewood, John L. Macomber, Seema Zaidi
Publikováno v:
Applied microbiology and biotechnology. 74(1)
A unique multifunctional glycosyl hydrolase was discovered by screening an environmental DNA library prepared from a microbial consortium collected from cow rumen. The protein consists of two adjacent catalytic domains. Sequence analysis predicted th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8c7fd492a19f5150562b00ff7631280
https://pubmed.ncbi.nlm.nih.gov/17103163
https://pubmed.ncbi.nlm.nih.gov/17103163