Zobrazeno 1 - 10
of 26
pro vyhledávání: '"John K. Koeppe"'
Autor:
Mary D. Shearin, Eric A. Whitsel, Richard C. Rayne, Deborah J. Carver, John K. Koeppe, Robert C. Vogler
Publikováno v:
Insect Biochemistry. 18:661-666
Hemolymph juvenile hormone-binding protein (JHBP) is synthesized and secreted from fat body in the adult female cockroach, Leucophaea maderae . The data in this paper suggest it is initially secreted from the fat body as a larger peptide whereas data
Publikováno v:
Journal of Insect Physiology. 26:749-753
Juvenile hormone (JH) synthesized and secreted in vitro by the corpora allata of mated adult Leucophaea maderae females was determined to be JH III (methyl-10,11-epoxy-3,7,11-trimethyl-2,6-dodecadienoate). The haemolymph titre of JH was determined du
Publikováno v:
Journal of Insect Physiology. 24:45-51
Several peptides have been found in the haemolymph which are antigenically similar to peptides found in the terminal oocyte during vitellogenesis. There appear to be two major peptides. labeled A and D, in the oocyte with a stoichiometry of A2D1. The
Autor:
Richard R. Mills, John K. Koeppe
Publikováno v:
Insect Biochemistry. 5:399-408
Carbinol- 14 C-noradrenalin and 2- 14 C-dopamine were injected into immature American cockroaches while in the process of shedding the old cuticle (ecdysis). In both cases, cuticles removed 8 hr later was found to have taken up the injected label. Pr
Autor:
Richard C. Rayne, John K. Koeppe
Publikováno v:
Insect Biochemistry. 18:667-673
Monoclonal antibodies (MAb) specific for the hemolymph 220 kD juvenile hormone-binding protein (JHBP) of the cockroach, Leucophaea maderae, were used to further characterize the JHBP. Spleen cells, from mice immunized with semipurified hemolymph JHBP
Publikováno v:
Insect Biochemistry. 5:223-229
The injection of carbinol- 14 C-noradrenalin into adult female American cockroaches resulted in the formation of labelled protocatechuic acid glucoside (3-hydroxy-4-0-β- d glucosylbenzoic acid). The glucoside was isolated metabolites were also ident
Autor:
Gae E. Kovalick, John K. Koeppe
Publikováno v:
Molecular and Cellular Endocrinology. 31:271-286
We modified a binding assay using polyethylene glycol (PEG) to precipitate bound hormone. Optimum precipitation occurred when reaction mixtures were incubated with 10–40% PEG and 1.25–2.5 mg/ml γ-globulins for 2–90 min at 4 or 23°C. Results f
Publikováno v:
Journal of Insect Physiology. 31:187-193
Microspectrophotometric analysis of Feulgen-stained nuclei of the terminal follicle cells in the cockroach Leucophaea maderae showed that during maturation the follicle cells became polyploid. In virgin females, the follicle cell nuclei were diploid.
Publikováno v:
Journal of Insect Physiology. 27:281-291
Changes in follicle cell morphology were correlated with changes in rates of protein synthesis and DNA synthesis by the ovary during ovarian maturation in Leucophaea maderae. During the vitellogenic period of oocyte development, which lasts approx, 1
Publikováno v:
Journal of Biological Chemistry. 259:3219-3223
A tritium-labeled diazocarbonyl juvenile hormone (JH) analog, (10-[10,11-3H]epoxyfarnesyl diazoacetate, [3H]EFDA), covalently bound to proteins in both hemolymph and ovarian extracts when reaction mixtures were irradiated with UV light. The addition