Zobrazeno 1 - 10
of 35
pro vyhledávání: '"John J. Macklin"'
Autor:
Jian-Zhong Guo, Austin R Graves, Wendy W Guo, Jihong Zheng, Allen Lee, Juan Rodríguez-González, Nuo Li, John J Macklin, James W Phillips, Brett D Mensh, Kristin M Branson, Adam W Hantman
Publikováno v:
eLife, Vol 5 (2016)
Externí odkaz:
https://doaj.org/article/5c86566ffac7461fb45c0a20ad009b45
Autor:
Hod Dana, Boaz Mohar, Yi Sun, Sujatha Narayan, Andrew Gordus, Jeremy P Hasseman, Getahun Tsegaye, Graham T Holt, Amy Hu, Deepika Walpita, Ronak Patel, John J Macklin, Cornelia I Bargmann, Misha B Ahrens, Eric R Schreiter, Vivek Jayaraman, Loren L Looger, Karel Svoboda, Douglas S Kim
Publikováno v:
eLife, Vol 5 (2016)
Genetically encoded calcium indicators (GECIs) allow measurement of activity in large populations of neurons and in small neuronal compartments, over times of milliseconds to months. Although GFP-based GECIs are widely used for in vivo neurophysiolog
Externí odkaz:
https://doaj.org/article/ab34ae2c5e2b47b2a8b288f3d69d627e
Autor:
Loren L. Looger, Douglas S. Kim, Eric R. Schreiter, Allan M. Wong, Chi-Yu Lee, Abhi Aggarwal, Jeong Jun Kim, Emiliano Jimenez Marquez, Allison M. Ahrens, Jonathan S. Marvin, Kaspar Podgorski, Yajie Liang, Daniel Bushey, Manuel A. Mohr, John J. Macklin, Getahun Tsegaye, Jerry L. Chen, Ronak Patel, Arthur Tsang
Publikováno v:
Nature methods
Femtosecond lasers at fixed wavelengths above 1,000 nm are powerful, stable and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a94b25e4a9d2307c74937c2fa6c0bb3e
https://doi.org/10.1038/s41592-020-0835-7
https://doi.org/10.1038/s41592-020-0835-7
Autor:
Jian-Zhong Guo, Austin R Graves, Wendy W Guo, Jihong Zheng, Allen Lee, Juan Rodríguez-González, Nuo Li, John J Macklin, James W Phillips, Brett D Mensh, Kristin Branson, Adam W Hantman
Publikováno v:
eLife, Vol 4 (2015)
Mammalian cerebral cortex is accepted as being critical for voluntary motor control, but what functions depend on cortex is still unclear. Here we used rapid, reversible optogenetic inhibition to test the role of cortex during a head-fixed task in wh
Externí odkaz:
https://doaj.org/article/ca20b66a26f24c9f97fae52732de8723
Autor:
Liam Paninski, Eric R. Schreiter, Bei Jung Lin, Takashi Kawashima, Ahmed S. Abdelfattah, Tsai Wen Chen, Ondrej Novak, Misha B. Ahrens, Gabe J. Murphy, Jihong Zheng, Karel Svoboda, Stephanie C. Seeman, Minoru Koyama, Jonathan B. Grimm, Yi Chieh Huang, Luke Campagnola, Jianing Yu, Johannes Friedrich, Ronak Patel, Amrita Singh, Yichun Shuai, Glenn C. Turner, Luke D. Lavis, Hui Liu, Kaspar Podgorski, John J. Macklin, Brett D. Mensh, Zhe Liu
Publikováno v:
Science. 365:699-704
Visualizing neuronal activity in vivo Imaging the changes in fluorescence of voltage-sensitive reagents would enable monitoring of the activity of neurons in vivo. Abdelfattah et al. created such a voltage indicator by designing a protein that combin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da6f1c68f1556fed66e0f4dab260e110
https://doi.org/10.1126/science.aav6416
https://doi.org/10.1126/science.aav6416
Autor:
Jasper eAkerboom, Nicole eCarreras Calderón, Lin eTian, Sebastian eWabnig, Matthias ePrigge, Johan eTolö, Andrew eGordus, Michael B Orger, Kristen E Severi, John J Macklin, Ronak ePatel, Stefan R Pulver, Trevor J Wardill, Elisabeth eFischer, Christina eSchüler, Tsai-Wen eChen, Karen S Sarkisyan, Jonathan S Marvin, Cornelia I Bargmann, Douglas S Kim, Sebastian eKügler, Leon eLagnado, Peter eHegemann, Alexander eGottschalk, Eric R Schreiter, Loren L Looger
Publikováno v:
Frontiers in Molecular Neuroscience, Vol 6 (2013)
Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Here we describe red, single-wavelength GECIs, RCaMPs, engineered from circular permutation of the thermostable red fluorescent protein mRuby. High-resolution
Externí odkaz:
https://doaj.org/article/46e541bc41ce4dd2bdcb29ec64a7b4d7
Autor:
Chi-Yu Lee, Abhi Aggarwal, John J. Macklin, Allan M. Wong, Eric R. Schreiter, Kaspar Podgorski, Jonathan S. Marvin, Daniel Bushey, Emiliano Jimenez Marquez, Manuel A. Mohr, Douglas S. Kim, Loren L. Looger, Yajie Liang, Ronak Patel
State-of-the-art GFP-based calcium indicators do not undergo efficient two-photon excitation at wavelengths above 1000 nm, for which inexpensive and powerful industrial femtosecond lasers are available. Here we report jYCaMP1, a yellow variant of jGC
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90a64867a18858ea307cf80416cb6369
https://doi.org/10.1101/816660
https://doi.org/10.1101/816660
Autor:
Michael Z. Lin, Younghee Oh, Ho Leung Ng, Michelle A. Baird, Douglas S. Kim, Niloufar Ataie, Mark A. Kay, Jun Chu, Reto Fiolka, Erik S. Welf, Benjamin Kim, Ryohei Yasuda, Alex Sens, Clement Tran Tang, Michael W. Davidson, Feijie Zhang, Michelle Hu, Kevin M. Dean, Tal Laviv, John J. Macklin, Hod Dana
Publikováno v:
Nature biotechnology
Orange-red fluorescent proteins (FPs) are widely used in biomedical research for multiplexed epifluorescence microscopy with GFP-based probes, but their different excitation requirements make multiplexing with new advanced microscopy methods difficul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::713b1194f39b47d59287a68f6937d089
https://doi.org/10.1038/nbt.3550
https://doi.org/10.1038/nbt.3550
Autor:
Luke D. Lavis, Hui Liu, Kaspar Podgorski, Liam Paninski, Ronak Patel, Brett D. Mensh, Tsai Wen Chen, Zhe Liu, Jonathan B. Grimm, Yi Chieh Huang, Minoru Koyama, Yichun Shuai, Bei Jung Lin, Karel Svoboda, Takashi Kawashima, Glenn C. Turner, Eric R. Schreiter, Misha B. Ahrens, Amrita Singh, Ondrej Novak, John J. Macklin, Johannes Friedrich, Ahmed S. Abdelfattah
Imaging changes in membrane potential using genetically encoded fluorescent voltage indicators (GEVIs) has great potential for monitoring neuronal activity with high spatial and temporal resolution. Brightness and photostability of fluorescent protei
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::479d63fae3403067d57d69e8bfbc3304
Autor:
Boaz Mohar, Karel Svoboda, Loren L. Looger, Hod Dana, Ronak Patel, Brad K. Hulse, Douglas S. Kim, Vivek Jayaraman, Eric R. Schreiter, Allan M. Wong, Aaron M. Kerlin, John J. Macklin, Arthur Konnerth, Yang Chen, Yi Sun, Jeremy P. Hasseman, Getahun Tsegaye, Arthur Tsang
Publikováno v:
Nature methods. 16(7)
Calcium imaging with genetically encoded calcium indicators (GECIs) is routinely used to measure neural activity in intact nervous systems. GECIs are frequently used in one of two different modes: to track activity in large populations of neuronal ce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7cb869db604edb3203371040bc2f257f
https://pubmed.ncbi.nlm.nih.gov/31209382
https://pubmed.ncbi.nlm.nih.gov/31209382