Zobrazeno 1 - 10
of 102
pro vyhledávání: '"John J. Finer"'
Publikováno v:
Journal of Microbiology & Biology Education, Vol 17, Iss 3, Pp 444-450 (2016)
In this investigation, the students’ goal was to isolate and characterize Agrobacterium strains from soil. Following selection and enrichment on 1A-t medium, putative Agrobacterium isolates were characterized by Gram stain reaction and biochemical
Externí odkaz:
https://doaj.org/article/51417f374a254d8180c6c5ecff5552a5
Autor:
Eric A, Dean, John J, Finer
Publikováno v:
Plant cell reports.
We characterize GFP expression driven by a soybean glycinin promoter in transgenic soybean. We demonstrate specific amino acid-mediated induction of this promoter in developing soybean seeds in vitro. In plants, gene expression is primarily regulated
Publikováno v:
PLoS ONE, Vol 11, Iss 11, p e0166074 (2016)
Introns, especially the first intron in the 5' untranslated region (5'UTR), can significantly impact gene expression via intron-mediated enhancement (IME). In this study, we demonstrate the leader intron of a soybean elongation factor 1A (eEF1A) gene
Externí odkaz:
https://doaj.org/article/39fd2be8151c4ffb975dd26e3e1a33a9
Autor:
Jixiang Kong, Paula Olhoft, Dhiraj Thakare, Lou Ann Batts, Bradford Rush, Susana Martin-Ortigosa, Maarten Stuiver, Oliver Schmitz, Nuananong Orchard, Andika Gunadi, David Pacheco-Villalobos, John J. Finer
Publikováno v:
Frontiers in Plant Science
Frontiers in Plant Science, Vol 11 (2020)
Frontiers in Plant Science, Vol 11 (2020)
1AbstractSuccessful regeneration of genetically modified plants from cell culture is highly dependent on the species, genotype, and tissue-type being targeted for transformation. Studies in some plant species have shown that when expression is altere
Publikováno v:
Plant Biotechnology Journal
Summary Cis‐regulatory elements in promoters are major determinants of binding specificity of transcription factors (TFs) for transcriptional regulation. To improve our understanding of how these short DNA sequences regulate gene expression, synthe
Publikováno v:
Planta. 245:849-860
Specific sequences within the leader intron of a soybean polyubiquitin gene stimulated gene expression when placed either within a synthetic intron or upstream of a core promoter. The intron in the 5′ untranslated region of the soybean polyubiquiti
Publikováno v:
Pest Management Science. 73:1229-1235
BACKGROUND Studies on plant–insect interactions of the soybean aphid, Aphis glycines (Matsumura), can be influenced by environmental fluctuations, status of the host plant and variability in microbial populations. Maintenance of aphids on in vitro-
Isolation and Characterization of Agrobacterium Strains from Soil: A Laboratory Capstone Experience†
Publikováno v:
Journal of Microbiology & Biology Education
Journal of Microbiology & Biology Education, Vol 17, Iss 3 (2016)
Journal of Microbiology & Biology Education, Vol 17, Iss 3, Pp 444-450 (2016)
Journal of Microbiology & Biology Education, Vol 17, Iss 3 (2016)
Journal of Microbiology & Biology Education, Vol 17, Iss 3, Pp 444-450 (2016)
In this investigation, the students’ goal was to isolate and characterize Agrobacterium strains from soil. Following selection and enrichment on 1A-t medium, putative Agrobacterium isolates were characterized by Gram stain reaction and biochemical
Autor:
John J. Finer
Publikováno v:
Current Protocols in Plant Biology
This protocol describes one method for generating transgenic soybean (Glycine max) using particle bombardment of embryogenic cultures. Embryogenic cultures consist of proliferating masses of somatic embryos and are initially obtained from the cotyled
Publikováno v:
Plant Cell, Tissue and Organ Culture (PCTOC). 127:145-160
Gene expression in plants is primarily modulated by the promoter region, which is located upstream of the gene coding region. Specific regulatory sequences within the promoter region contribute to the strength, tissue specificity, timing and duration