Expression of three mammalian cDNAs that interfere with RAS function in Saccharomyces cerevisiae

Autor: Michael Wigler, Michael Riggs, C. Birchmeier, Linda Rodgers, John J. Colicelli, Charles Nicolette

Publikováno v: Scopus-Elsevier

Saccharomyces cerevisiae strains expressing the activated RAS2Val19 gene or lacking both cAMP phosphodiesterase genes, PDE1 and PDE2, have impaired growth control and display an acute sensitivity to heat shock. We have isolated two classes of mammali

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a177b92ff7a952802620f7c7f0256810
https://doi.org/10.1073/pnas.88.7.2913

Cloning and characterization of CAP, the S. cerevisiae gene encoding the 70 kd adenylyl cyclase-associated protein

Autor: Michael Riggs, K. Ferguson, Jeffrey E. Gerst, Jeffrey Field, R. Ballester, Scott Powers, Graeme Bolger, Anne Vojtek, T. Michaeli, Tohru Kataoka, B. Wheland, Michael Wigler, Linda Rodgers, Ilse Wieland, John J. Colicelli

Publikováno v: Scopus-Elsevier

Adenylyl cyclase from S. cerevisiae contains at least two subunits, a 200 kd catalytic subunit and a subunit with an apparent molecular size of 70 kd, which we now call CAP (cyclase-associated protein). We cloned a cDNA encoding CAP by screening a ye

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f67d499281354c5261146045958d17c
https://doi.org/10.1016/0092-8674(90)90812-s

Therapeutic Insights From a Novel Breast Tumor Suppressor Pathway

Autor: John J. Colicelli

Epigenetic gene silencing is a common element of breast cancers. The specific genes targeted and the mechanisms of silencing, and how these relate to disease progression and outcome, are not well understood. This research project has 1) identified a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::97461f3a4ceb08a618efb6b0c7628c54
https://doi.org/10.21236/ada437102

Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase

Autor: C. Birchmeier, T. Michaeli, Michael Wigler, Kathy O'Neill, John J. Colicelli, Michael Riggs

Publikováno v: Scopus-Elsevier

A rat brain cDNA library has been constructed in a Saccharomyces cerevisiae expression vector and used to isolate genes that can function in yeast to suppress the phenotypic effects of RAS2val19, a mutant form of the RAS2 gene analogous to an oncogen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::449f36a19b7df5cdd88a479f73ea7fc9
http://www.scopus.com/inward/record.url?eid=2-s2.0-0024670853&partnerID=MN8TOARS

Tyrosine phosphorylation of RAS by ABL allosterically enhances effector binding.

Autor: Ting PY; Molecular Biology Institute, Jonsson Comprehensive Cancer Center, Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California, USA; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA; and Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California at Los Angeles Metabolomics and Proteomics Center, California Nanosystems Institute and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, California, USA., Johnson CW; Molecular Biology Institute, Jonsson Comprehensive Cancer Center, Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California, USA; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA; and Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California at Los Angeles Metabolomics and Proteomics Center, California Nanosystems Institute and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, California, USA., Fang C; Molecular Biology Institute, Jonsson Comprehensive Cancer Center, Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California, USA; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA; and Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California at Los Angeles Metabolomics and Proteomics Center, California Nanosystems Institute and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, California, USA., Cao X; Molecular Biology Institute, Jonsson Comprehensive Cancer Center, Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California, USA; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA; and Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California at Los Angeles Metabolomics and Proteomics Center, California Nanosystems Institute and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, California, USA., Graeber TG; Molecular Biology Institute, Jonsson Comprehensive Cancer Center, Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California, USA; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA; and Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California at Los Angeles Metabolomics and Proteomics Center, California Nanosystems Institute and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, California, USA., Mattos C; Molecular Biology Institute, Jonsson Comprehensive Cancer Center, Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California, USA; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA; and Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California at Los Angeles Metabolomics and Proteomics Center, California Nanosystems Institute and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, California, USA., Colicelli J; Molecular Biology Institute, Jonsson Comprehensive Cancer Center, Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California, USA; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA; and Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California at Los Angeles Metabolomics and Proteomics Center, California Nanosystems Institute and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, California, USA colicelli@mednet.ucla.edu.

Publikováno v: FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2015 Sep; Vol. 29 (9), pp. 3750-61. Date of Electronic Publication: 2015 May 21.