Zobrazeno 1 - 10
of 70
pro vyhledávání: '"John J. Buckley"'
Publikováno v:
Environmental Research Letters, Vol 10, Iss 12, p 124005 (2015)
Interbasin water transfers are globally important water management strategies, yet little is known about their role in the hydrologic cycle at regional and continental scales. Specifically, there is a dearth of centralized information on transfer loc
Externí odkaz:
https://doaj.org/article/f38c391c3ce14795945ba8d1434d8d97
Publikováno v:
Journal of Membrane Science. 490:294-300
Ultrafiltration is widely used for the purification of polysaccharide-based vaccines against pneumococci and meningococci, but there is currently no fundamental understanding of the factors controlling the ultrafiltration behavior of these polysaccha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e5c2e695c9f6a592c077fb933300d2a0
https://doi.org/10.1016/j.memsci.2015.04.047
https://doi.org/10.1016/j.memsci.2015.04.047
Autor:
Kaley L. Meadows, John J. Buckley
Publikováno v:
Journal of Healthcare Management. 59:173-176
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6d593e69c40ae5b0448b4af72e9e17dc
https://doi.org/10.1097/00115514-201405000-00004
https://doi.org/10.1097/00115514-201405000-00004
Publikováno v:
Biotechnology progress. 32(6)
Charged polysaccharides are used in the food industry, as cosmetics, and as vaccines. The viscosity, thermodynamics, and hydrodynamic properties of these charged polysaccharides are known to be strongly dependent on the solution ionic strength becaus
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbc458457ec2907a305f133a1f0b23f4
https://pubmed.ncbi.nlm.nih.gov/27673326
https://pubmed.ncbi.nlm.nih.gov/27673326
Publikováno v:
Carbohydrate polymers. 152
Molecular properties of bacterial polysaccharides and protein-polysaccharide conjugates play an important role in the efficiency and immunogenicity of the final vaccine product. Size exclusion chromatography (SEC) is commonly used to analyze and char
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::639eb6482c907bbf2ce66d6fc966c1c3
https://pubmed.ncbi.nlm.nih.gov/27516244
https://pubmed.ncbi.nlm.nih.gov/27516244
Autor:
Kevin Wamiti Pfizer Global Res. Dev. Muthurania, Gopinath Vedachalam Annathur, Natrajan Ramasubramanyan, John J. Buckley
Publikováno v:
Journal of Chromatography A. 1217:3783-3793
A cation exchange chromatographic purification process step was developed for the purification of human PEGylated PYY 3-36 from the PEGylation reaction mixture. In this publication we describe experiments carried out to evaluate the chromatographic p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae64fa2f8462925042d26e157b030dd8
https://doi.org/10.1016/j.chroma.2010.04.019
https://doi.org/10.1016/j.chroma.2010.04.019
Publikováno v:
Biotechnology and Applied Biochemistry. 56:27-34
A scalable refold for human neurotrophin-4 was developed as part of a manufacturing process required for the production of supplies for preclinical and clinical studies. The process redox system, chaotrope, solubilization additives, pH, temperature a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9f9639886f679c2f8e06fc1f9462a11
https://doi.org/10.1042/ba20090306
https://doi.org/10.1042/ba20090306
Publikováno v:
Biotechnology and Applied Biochemistry. 54:85-92
Optimization of refolding conditions for progenipoietin was performed. The molecule has five disulfide bonds and, hence, is a challenge to refold. Variables studied included pH, DTT (dithiothreitol) concentration, cystine concentration, urea concentr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54bbd8baad318d47dd81fcdee5f0d78b
https://doi.org/10.1042/ba20080268
https://doi.org/10.1042/ba20080268
Publikováno v:
Journal of Chromatography A. 1147:172-182
We have studied the effect of protein PEGylation on ion-exchange adsorption using bovine serum albumin as a model system. The free sulfhydryl group of BSA, located on cysteine 34, was PEGylated using the maleimido-PEG chemistry. Several different BSA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64b84d1eecb1c63a1fe36c22638b98df
https://doi.org/10.1016/j.chroma.2007.02.051
https://doi.org/10.1016/j.chroma.2007.02.051
Publikováno v:
Biotechnology and applied biochemistry. 37(Pt 1)
Process development and characterization studies were performed for the chromatographic steps in the purification process of a recombinant Escherichia coli -expressed protein product candidate. The objective of this work was to develop a robust and e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e4102ae7c72b97f36167a26f458b636
https://pubmed.ncbi.nlm.nih.gov/12578552
https://pubmed.ncbi.nlm.nih.gov/12578552