Essential metabolism for a minimal cell

Autor: Marian Breuer, Tyler M Earnest, Chuck Merryman, Kim S Wise, Lijie Sun, Michaela R Lynott, Clyde A Hutchison, Hamilton O Smith, John D Lapek, David J Gonzalez, Valérie de Crécy-Lagard, Drago Haas, Andrew D Hanson, Piyush Labhsetwar, John I Glass, Zaida Luthey-Schulten

Publikováno v: eLife, Vol 8 (2019)

JCVI-syn3A, a robust minimal cell with a 543 kbp genome and 493 genes, provides a versatile platform to study the basics of life. Using the vast amount of experimental information available on its precursor, Mycoplasma mycoides capri, we assembled a

Externí odkaz: https://doaj.org/article/8a4a0da0c3184384beb1ab8154468210

A genome-scale metabolic reconstruction of Mycoplasma genitalium, iPS189.

Autor: Patrick F Suthers, Madhukar S Dasika, Vinay Satish Kumar, Gennady Denisov, John I Glass, Costas D Maranas

Publikováno v: PLoS Computational Biology, Vol 5, Iss 2, p e1000285 (2009)

With a genome size of approximately 580 kb and approximately 480 protein coding regions, Mycoplasma genitalium is one of the smallest known self-replicating organisms and, additionally, has extremely fastidious nutrient requirements. The reduced geno

Externí odkaz: https://doaj.org/article/28e845ac3ba54dd3aaccd8a178df7a32

Minimal cell JCVI-syn3B as a chassis to reveal the mechanisms behind Mycoplasma host–pathogen interactions

Autor: Daniela M. de C. Bittencourt, David M. Brown, Nacyra Assad-Garcia, Michaela R. Lynott, Lijie Sun, Luis Alberto M. Palhares de Melo, Marcelo Freire, John I. Glass

Mycoplasmas are atypical bacteria that are obligate parasites of animals and plants. They are known for their capacity to contaminate and parasitize mammalian cell cultures, thereby offering fortuitous models to examine the host-microbe interaction.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2db1747f1f7f797da0b63ef86b098bae
https://doi.org/10.1101/2022.09.19.508583

Traditional protocols and optimization methods lead to absent expression in a mycoplasma cell-tree gene expression platform

Autor: Andrei Sakai, Christopher R Deich, Frank H T Nelissen, Aafke J Jonker, Daniela M de C Bittencourt, Christopher P Kempes, Kim S Wise, Hans A Heus, Wilhelm T S Huck, Katarzyna P Adamala, John I Glass

Publikováno v: Synthetic Biology, 7, 1, pp. 1-14
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice)
Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
Synthetic Biology, 7, 1-14

Cell-free expression (CFE) systems are one of the main platforms for building synthetic cells. A major drawback is the orthogonality of cell-free systems across species. To generate a CFE system compatible with recently established minimal cell const

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16305d9546f890b7a0497a9116429293

High-Content Screening and Computational Prediction Reveal Viral Genes That Suppress the Innate Immune Response

Autor: Tai L. Ng, Erika J. Olson, Tae Yeon Yoo, H. Sloane Weiss, Yukiye Koide, Peter D. Koch, Nathan J. Rollins, Pia Mach, Tobias Meisinger, Trenton Bricken, Timothy Z. Chang, Colin Molloy, Jérôme Zürcher, Roger L. Chang, Timothy J. Mitchison, John I. Glass, Debora S. Marks, Jeffrey C. Way, Pamela A. Silver

Publikováno v: mSystems. 7(2)

Suppression of the host innate immune response is a critical aspect of viral replication. Upon infection, viruses may introduce one or more proteins that inhibit key immune pathways, such as the type I interferon pathway. However, the ability to pred

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3f0c5c4894a6bf36e89fd6c8b0ea6cc
https://pubmed.ncbi.nlm.nih.gov/35319251