Zobrazeno 1 - 10
of 61
pro vyhledávání: '"John Hubble"'
Publikováno v:
Journal of Membrane Science. 339:138-142
A novel method for the preparation of a composite pore-filled membrane based on self-assembled hydrogels is described. Responsive pore-filled membranes based on mixtures of dextran (molecular weight 6000) and the glucose binding lectin Concanavalin A
Publikováno v:
Advances in Polymer Technology. 27:27-34
A temperature-sensitive hydrogel based on a copolymer of BSA and poly(N-isopropylacrylamide) (PNIPAAm) has been synthesized using carbodiimide chemistry. Fourier transform infrared spectroscopy confirmed primary complex formation between carbodiimide
Autor:
John Hubble
Publikováno v:
Journal of Chemical Technology & Biotechnology. 36:487-493
The performance of a low cost differential enzyme thermistor system was studied. Initial results from physical characterisations showed the interdependence of operating parameters (flow rate and ambient temperature) on detector sensitivity. Subsequen
Publikováno v:
Reactive and Functional Polymers. 66:757-767
A facile method is described for the preparation of d -glucose-sensitive hydrogel membranes based on cross-linking carboxymethyl dextran (CM-dextran) with the glucose binding lectin concanavalin A (ConA) using carbodiimide chemistry. The mesh size of
Publikováno v:
Biotechnology Letters. 27:1587-1590
A facile method for the characterization of hydrogel swelling is described which is based on the determination of changes in the liquid phase concentration of an excluded tracer as gel swells in a constant volume system. The utility of this approach
Publikováno v:
Biomaterials. 26:4677-4683
A fast and simple method for the preparation of pH-sensitive hydrogel membranes for drug delivery and tissue engineering applications has been developed using carbodiimide chemistry. The hydrogels were formed by the intermolecular cross-linking of ca
Autor:
John Hubble, F. Santori
Publikováno v:
Separation Science and Technology. 40:1733-1748
Low affinity pair size exclusion chromatography (LAPSEC) is herein described as a novel approach specifically for lectin based monosaccharide separation but with the potential for wider application using weak antibody‐antigen interactions. This tec
Publikováno v:
Biotechnology and Bioengineering. 87:791-796
A hydrogel membrane containing immobilized ligands and receptors was synthesized and investigated for the controlled diffusion of test proteins (cytochrome C and hemoglobin). Both Cibacron blue (ligand) and lysozyme (receptor) were covalently linked
Autor:
John Hubble
Publikováno v:
Chemical Engineering Science. 58:4465-4474
A theoretical model is developed to describe the affinity-mediated interaction of a suspended cell population and a support surface. The model is capable of describing attachment and detachment phenomena, taking account of both bulk and surface effec
Autor:
F. Santori, John Hubble
Publikováno v:
Journal of Chromatography A. 1003:123-126
The successful fractionation of a mixture of monosaccharides using an immobilized lectin column operating under isocratic conditions is described. The key factors for effective separation were found to be sample size, feed velocity and column length.