Zobrazeno 1 - 10
of 33
pro vyhledávání: '"John Heptinstall"'
Publikováno v:
Dyes and Pigments. 99:160-167
Here we report that the difficulties encountered in the synthesis of a dual-pendant sulfonate bis(indolenine)squaraine dye can be overcome through the use of equimolar amounts of the common reaction catalyst, thus creating an organic salt between the
Autor:
Maria Gómez-Mingot, John Heptinstall, Vicente Montiel, Luis A. Alcaraz, Jesús Iniesta, Mario Piccioli, Antonio Donaire
Publikováno v:
Archives of Biochemistry and Biophysics. 529:26-33
Nitration in proteins is a physiologically relevant process and the formation of 3-nitrotyrosine was first proposed as an in vivo marker of the production of reactive nitrogen species in oxidative stress. No studies have been published on structural
Autor:
Victor A. Mikhailov, Jesús Iniesta, John Heptinstall, Martin Cox, Maria Gómez-Mingot, David J. Walton, Helen J. Cooper, Sadie Dean
Publikováno v:
Ultrasonics Sonochemistry. 18:334-344
Solutions containing hen egg white lysozyme (HEWL) and nitrite were exposed to ultrasonic irradiation in order to study the possible sonochemical modifications. This is the first demonstration of the nitration of tyrosine residues in a protein (lysoz
Autor:
Jesús Iniesta, María Deseada Esclapez-Vicente, Helen J. Cooper, Victor A. Mikhailov, John Heptinstall, David J. Walton, Ian R. Peterson
Publikováno v:
Enzyme and Microbial Technology
In this paper we report the successful use of a non-metallic electrode material, boron-doped diamond (BDD), for the anodic electro-oxidative modification of hen egg white lysozyme (HEWL). Platinum electrodes can give rise to loss of activity of HEWL
Autor:
Petra F. Mens, R. J. Matelon, John Heptinstall, Jamie Beddow, Henk D. F. H. Schallig, Dave M. Newman, Luke Savage, M. Lesley Wears, Martin Cox
Publikováno v:
Biophysical journal, 95(2), 994-1000. Biophysical Society
We report the development of magneto-optic technology for the rapid quantitative diagnosis of malaria that may also be realizable in a noninvasive format. Hemozoin, the waste product of malarial parasitic action on hemoglobin, is produced in a form t
Autor:
Dominic Matters, Helen J. Cooper, Ian R. Peterson, Liam A. McDonnell, David J. Walton, Peter J. Derrick, John Heptinstall, Jesus Iniesta
Publikováno v:
Analytical Biochemistry. 356:171-181
In producing a method for selective protein nitration, we previously demonstrated the electrochemical nitration of hen egg white lysozyme to be at Tyr23 initially, followed by bisnitration at Tyr20, but with no trisnitration at Tyr53. The nitration s
Autor:
M Plattes, Timothy J. Mason, S.S. Phull, H. Fuji, Shuntaro Mataka, John Heptinstall, S. Ryley, A. Chyla, Jesus Iniesta, J.P. Lorimer, Yasuko Tanaka, Thies Thiemann, David J. Walton
Publikováno v:
Ultrasonics Sonochemistry. 10:209-216
This paper describes recent studies in organic sonoelectrochemistry at Coventry University, including the oxidation of thiophene monoxides, degradation of dye pollutants, formation of conducting polymers and electrosynthetic modification of proteins.
Publikováno v:
Journal of Electroanalytical Chemistry. 544:107-112
The diffusion through an agarose hydrogel of the ionophoric antibiotic valinomycin dissolved in a mixture of water and ethanol is characterized by electrochemically monitoring the conductivity of a lipid bilayer membrane buried within the gel. Classi
Autor:
Georgina Kendall, Helen J. Cooper, David J. Walton, Peter J. Derrick, John Heptinstall, Ian R. Peterson
Publikováno v:
Archives of Biochemistry and Biophysics. 392:169-179
Earlier findings on electronitration of hen egg-white lysozyme demonstrated a product which was mononitrated at Tyr23, by ion-exchange chromatography, absorbance at 430 nm, dithionite reduction, and Edman sequencing of a nitrated proteolytic peptide.
Autor:
David J. Walton, John Heptinstall
Publikováno v:
Preparative Biochemistry and Biotechnology. 30:1-14
INTRODUCTION The majority of electrochemical studies of biomolecules have been analytical in nature, usually involving electron transfer by redox proteins. Synthetic applications of electrochemistry to proteins, leading to the production of recoverab