Zobrazeno 1 - 10
of 73
pro vyhledávání: '"John Heptinstall"'
Autor:
Maria Gómez-Mingot, John Heptinstall, Vicente Montiel, Luis A. Alcaraz, Jesús Iniesta, Mario Piccioli, Antonio Donaire
Publikováno v:
Archives of Biochemistry and Biophysics. 529:26-33
Nitration in proteins is a physiologically relevant process and the formation of 3-nitrotyrosine was first proposed as an in vivo marker of the production of reactive nitrogen species in oxidative stress. No studies have been published on structural
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df8d3ea15bc95dd45c0719978de2c4e8
https://doi.org/10.1016/j.abb.2012.10.013
https://doi.org/10.1016/j.abb.2012.10.013
Autor:
Victor A. Mikhailov, Jesús Iniesta, John Heptinstall, Martin Cox, Maria Gómez-Mingot, David J. Walton, Helen J. Cooper, Sadie Dean
Publikováno v:
Ultrasonics Sonochemistry. 18:334-344
Solutions containing hen egg white lysozyme (HEWL) and nitrite were exposed to ultrasonic irradiation in order to study the possible sonochemical modifications. This is the first demonstration of the nitration of tyrosine residues in a protein (lysoz
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aff476de3f0b6a262e0884828e8015b2
https://doi.org/10.1016/j.ultsonch.2010.06.013
https://doi.org/10.1016/j.ultsonch.2010.06.013
Autor:
Jesús Iniesta, María Deseada Esclapez-Vicente, Helen J. Cooper, Victor A. Mikhailov, John Heptinstall, David J. Walton, Ian R. Peterson
Publikováno v:
Enzyme and Microbial Technology
In this paper we report the successful use of a non-metallic electrode material, boron-doped diamond (BDD), for the anodic electro-oxidative modification of hen egg white lysozyme (HEWL). Platinum electrodes can give rise to loss of activity of HEWL
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27a3cc0ccf6d0eb7015aa6a6bc830c24
http://europepmc.org/articles/PMC3101334
http://europepmc.org/articles/PMC3101334
Autor:
Petra F. Mens, R. J. Matelon, John Heptinstall, Jamie Beddow, Henk D. F. H. Schallig, Dave M. Newman, Luke Savage, M. Lesley Wears, Martin Cox
Publikováno v:
Biophysical journal, 95(2), 994-1000. Biophysical Society
We report the development of magneto-optic technology for the rapid quantitative diagnosis of malaria that may also be realizable in a noninvasive format. Hemozoin, the waste product of malarial parasitic action on hemoglobin, is produced in a form t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::604465e409d105e354e3ffa3f668a9cf
https://doi.org/10.1529/biophysj.107.128140
https://doi.org/10.1529/biophysj.107.128140
Autor:
Dominic Matters, Helen J. Cooper, Ian R. Peterson, Liam A. McDonnell, David J. Walton, Peter J. Derrick, John Heptinstall, Jesus Iniesta
Publikováno v:
Analytical Biochemistry. 356:171-181
In producing a method for selective protein nitration, we previously demonstrated the electrochemical nitration of hen egg white lysozyme to be at Tyr23 initially, followed by bisnitration at Tyr20, but with no trisnitration at Tyr53. The nitration s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3000cd7668103b358a0d993ccdbcaa94
https://doi.org/10.1016/j.ab.2006.06.033
https://doi.org/10.1016/j.ab.2006.06.033
Autor:
M Plattes, Timothy J. Mason, S.S. Phull, H. Fuji, Shuntaro Mataka, John Heptinstall, S. Ryley, A. Chyla, Jesus Iniesta, J.P. Lorimer, Yasuko Tanaka, Thies Thiemann, David J. Walton
Publikováno v:
Ultrasonics Sonochemistry. 10:209-216
This paper describes recent studies in organic sonoelectrochemistry at Coventry University, including the oxidation of thiophene monoxides, degradation of dye pollutants, formation of conducting polymers and electrosynthetic modification of proteins.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8aa5b7520aeda9b06d4caecb472e47f7
https://doi.org/10.1016/s1350-4177(02)00153-0
https://doi.org/10.1016/s1350-4177(02)00153-0
Publikováno v:
Journal of Electroanalytical Chemistry. 544:107-112
The diffusion through an agarose hydrogel of the ionophoric antibiotic valinomycin dissolved in a mixture of water and ethanol is characterized by electrochemically monitoring the conductivity of a lipid bilayer membrane buried within the gel. Classi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2e2a98079bdbe9daf81bb7a9e184fbe7
https://doi.org/10.1016/s0022-0728(03)00083-4
https://doi.org/10.1016/s0022-0728(03)00083-4
Autor:
Georgina Kendall, Helen J. Cooper, David J. Walton, Peter J. Derrick, John Heptinstall, Ian R. Peterson
Publikováno v:
Archives of Biochemistry and Biophysics. 392:169-179
Earlier findings on electronitration of hen egg-white lysozyme demonstrated a product which was mononitrated at Tyr23, by ion-exchange chromatography, absorbance at 430 nm, dithionite reduction, and Edman sequencing of a nitrated proteolytic peptide.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd5a9ba511d2367d6abfe99c956b38f9
https://doi.org/10.1006/abbi.2001.2451
https://doi.org/10.1006/abbi.2001.2451
Autor:
David J. Walton, John Heptinstall
Publikováno v:
Preparative Biochemistry and Biotechnology. 30:1-14
INTRODUCTION The majority of electrochemical studies of biomolecules have been analytical in nature, usually involving electron transfer by redox proteins. Synthetic applications of electrochemistry to proteins, leading to the production of recoverab
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d317955d01d00934c9a66f54803bd0a1
https://doi.org/10.1080/10826060008544939
https://doi.org/10.1080/10826060008544939
Publikováno v:
Biosensors and Bioelectronics. 14:265-271
We report improved techniques for the fabrication of biomimetic membranes protectively sandwiched between two slabs of agarose gel and the evaluation of their characteristics relevant for use in a biosensor based on gated channel proteins. The gel pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::34179f418c605b33ed70c90252368e9e
https://doi.org/10.1016/s0956-5663(99)00003-2
https://doi.org/10.1016/s0956-5663(99)00003-2