Automated analysis of NF-κB nuclear translocation kinetics in high-throughput screening.

Autor: Zi Di, Bram Herpers, Lisa Fredriksson, Kuan Yan, Bob van de Water, Fons J Verbeek, John H N Meerman

Publikováno v: PLoS ONE, Vol 7, Iss 12, p e52337 (2012)

Nuclear entry and exit of the NF-κB family of dimeric transcription factors plays an essential role in regulating cellular responses to inflammatory stress. The dynamics of this nuclear translocation can vary significantly within a cell population a

Externí odkaz: https://doaj.org/article/76f7747a52b841dda8a95e82c5398888

IGF1R signaling drives antiestrogen resistance through PAK2/PIX activation in luminal breast cancer

Autor: Lynn Wester, Marjo de Graauw, Tamar Geiger, John W.M. Martens, Yinghui Zhang, Chantal Pont, Bob van de Water, Jean A. Helmijr, Maurice P.H.M. Jansen, Maxime P. Look, Marja Moerkens, Caroline H M van Deurzen, John H. N. Meerman, Els M.J.J. Berns, Erik H.J. Danen, Mieke M. Timmermans, Ram Siddappa, Jichao He

Publikováno v: Oncogene, 37(14), 1869-1884. Nature Publishing Group

Antiestrogen resistance in estrogen receptor positive (ER+) breast cancer is associated with increased expression and activity of insulin-like growth factor 1 receptor (IGF1R). Here, a kinome siRNA screen has identified 10 regulators of IGF1R-mediate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::474be77ac75a490aa422f25e9f8034a7
https://hdl.handle.net/1887/3202823

The Nuclear FactorκB Family Member RelB Facilitates Apoptosis of Renal Epithelial Cells Caused by Cisplatin/Tumor Necrosis FactorαSynergy by Suppressing an Epithelial to Mesenchymal Transition–Like Phenotypic Switch

Autor: Bram Herpers, Lisa Fredriksson, Kuan Yan, Marjo de Graauw, Michiel Fokkelman, John H. N. Meerman, Bob van de Water, Giulia Benedetti

Publikováno v: Molecular Pharmacology. 84:128-138

Cis-diamminedichloroplatinum(II) (cisplatin)-induced renal proximal tubular apoptosis is known to be preceded by actin cytoskeleton reorganization, in conjunction with disruption of cell-matrix and cell-cell adhesion. In the present study, we show th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f59f6a29decbbd827fe4a930aab9a86
https://doi.org/10.1124/mol.112.084053

Some nonylphenol isomers show antiestrogenic potency in the MVLN cell assay

Autor: Hans Toni Ratte, John H. N. Meerman, Hande Gurer-Orhan, Thomas G. Preuss

Publikováno v: Toxicology in Vitro. 24:129-134

It has been shown that nonylphenol (NP) isomers vary in their estrogenic potency. These differences may be due to varieties in receptor affinity, receptor activation, or activation/deactivation of non-receptor mediated side paths of reporter gene tra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55aad36cc1cb51a49d6ee6e82e608fc1
https://doi.org/10.1016/j.tiv.2009.08.017

Metabolic profiling of endocrine-disrupting compounds by on-line cytochrome p450 bioreaction coupled to on-line receptor affinity screening

Autor: Sebastiaan M. van Liempd, Jeroen Kool, Hubertus Irth, Nico P. E. Vermeulen, John H. N. Meerman

Publikováno v: Chemical Research in Toxicology, 20(12), 1825-32. American Chemical Society
Van Liempd, S M, Kool, J, Meerman, J H, Irth, H & Vermeulen, N P 2007, ' Metabolic profiling of endocrine-disrupting compounds by
cytochrome p450 bioreaction coupled to
receptor affinity screening ', Chemical Research in Toxicology, vol. 20, no. 12, pp. 1825-32 . https://doi.org/10.1021/tx7000724

We present a fully automated and hyphenated bioanalytical method for metabolic profiling of potentially harmful xenoestrogens. The system consists of an on-line cytochrome P450 bioreactor coupled to a reversed-phase, gradient high-performance liquid

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3cc6dca3bdf101922073998d77731354
https://hdl.handle.net/1871.1/98a0f0f3-e8eb-445b-984e-3c27b75ac6fa