Zobrazeno 1 - 10
of 29
pro vyhledávání: '"John H, Leamon"'
Publikováno v:
BioTechniques, Vol 28, Iss 5, Pp 994-1005 (2000)
A method is described that uses the ABI PRISMTM 310 genetic analyzer in conjunction with custom-designed software to identify and classify RAPD products. This methodology will also work well with AFLPs and microsatellite analyses. The methodology use
Externí odkaz:
https://doaj.org/article/96ffa7f7a1cf44d8810011a95156a645
Autor:
Michael Thornton, Carol Beadling, John H. Leamon, Michael Heinrich, Mark Andersen, Kate Rhodes, Christopher L. Corless, Tanaya Neff
Publikováno v:
The Journal of Molecular Diagnostics. 15:171-176
There is growing demand for routine identification of actionable mutations in clinical cancer specimens. Genotyping platforms must provide rapid turnaround times and work effectively with limited amounts of formalin-fixed, paraffin-embedded (FFPE) ti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a5824b57b8cd616b1d73bea12c72b39
https://doi.org/10.1016/j.jmoldx.2012.09.003
https://doi.org/10.1016/j.jmoldx.2012.09.003
Publikováno v:
Gene Therapy and Regulation. :15-31
A challenge to the realization of the vision of personalized medicine is our lack of understanding of the amount of genetic variation contained in the human species, which has been prohibitively expensive to acquire to date. In this review, we discus
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::096d013aeb49a8312c374c83444c2f4e
https://doi.org/10.1142/s1568558607000046
https://doi.org/10.1142/s1568558607000046
Autor:
Jeffrey, Sabina, John H, Leamon
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1347
Whole genome amplification (WGA) is a widely used molecular technique that is becoming increasingly necessary in genetic research on a range of sample types including individual cells, fossilized remains and entire ecosystems. Multiple methods of WGA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::8f736c012877e006b00d92cc4120739f
https://pubmed.ncbi.nlm.nih.gov/26374307
https://pubmed.ncbi.nlm.nih.gov/26374307
Autor:
Jeffrey Sabina, John H. Leamon
Publikováno v:
Whole Genome Amplification ISBN: 9781493929894
Whole genome amplification (WGA) is a widely used molecular technique that is becoming increasingly necessary in genetic research on a range of sample types including individual cells, fossilized remains and entire ecosystems. Multiple methods of WGA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2e3997cf46f85f16c02fb1258d909c15
https://doi.org/10.1007/978-1-4939-2990-0_2
https://doi.org/10.1007/978-1-4939-2990-0_2
Autor:
Marcel, Margulies, Michael, Egholm, William E, Altman, Said, Attiya, Joel S, Bader, Lisa A, Bemben, Jan, Berka, Michael S, Braverman, Yi-Ju, Chen, Zhoutao, Chen, Scott B, Dewell, Lei, Du, Joseph M, Fierro, Xavier V, Gomes, Brian C, Godwin, Wen, He, Scott, Helgesen, Chun Heen, Ho, Chun He, Ho, Gerard P, Irzyk, Szilveszter C, Jando, Maria L I, Alenquer, Thomas P, Jarvie, Kshama B, Jirage, Jong-Bum, Kim, James R, Knight, Janna R, Lanza, John H, Leamon, Steven M, Lefkowitz, Ming, Lei, Jing, Li, Kenton L, Lohman, Hong, Lu, Vinod B, Makhijani, Keith E, McDade, Michael P, McKenna, Eugene W, Myers, Elizabeth, Nickerson, John R, Nobile, Ramona, Plant, Bernard P, Puc, Michael T, Ronan, George T, Roth, Gary J, Sarkis, Jan Fredrik, Simons, John W, Simpson, Maithreyan, Srinivasan, Karrie R, Tartaro, Alexander, Tomasz, Kari A, Vogt, Greg A, Volkmer, Shally H, Wang, Yong, Wang, Michael P, Weiner, Pengguang, Yu, Richard F, Begley, Jonathan M, Rothberg
Publikováno v:
Nature. 437:376-380
The proliferation of large-scale DNA-sequencing projects in recent years has driven a search for alternative methods to reduce time and cost. Here we describe a scalable, highly parallel sequencing system with raw throughput significantly greater tha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be63f61c869490283189d10b7b7d43b2
https://doi.org/10.1038/nature03959
https://doi.org/10.1038/nature03959
Publikováno v:
Journal of Aquatic Animal Health. 13:246-256
In fall 1998, a sample of 716 mummichogs Fundulus heteroclitus was collected from seven Connecticut salt marshes as part of an estuary biomonitoring program. The collection sites consisted of one relatively unpolluted reference site and six others wi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7eaae160894e654b33e60edba6d99d05
https://doi.org/10.1577/1548-8667(2001)013<0246:vipbam>2.0.co
https://doi.org/10.1577/1548-8667(2001)013<0246:vipbam>2.0.co
Publikováno v:
Journal of Aquatic Animal Health. 13:276-279
We present a new technique for determining RNA:DNA ratios in larval and juvenile fish that relies on the rapid homogenization of samples with salts, detergents, and proteinase K and the detection of DNA and RNA with specific fluorescent dyes. Samples
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3cb013cfb3f1670d128eb4dd6ec8391a
https://doi.org/10.1577/1548-8667(2001)013<0276:tdoara>2.0.co
https://doi.org/10.1577/1548-8667(2001)013<0276:tdoara>2.0.co
Autor:
Gemma Tarafa, Todd Strugnell, Daniela Ladner, John H. Leamon, Stefan Hamann, Deborah A. Dillon, Jose Costa, Paul M. Lizardi
Publikováno v:
Scopus-Elsevier
Detection of somatic low abundance mutations in early cancer development requires a discriminatory, specific, and high-throughput methodology. In this study we report specific, discriminatory detection of low abundance mutations through a novel combi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9cd146d3cfb1e73f8564fae6288f3816
https://doi.org/10.1038/labinvest.3780320
https://doi.org/10.1038/labinvest.3780320
Autor:
John H. Leamon, Yi Zhou, Tod Strugnell, Stefan Hamann, Matthew W. Christian, Margaret Calciano, Paul M. Lizardi
Publikováno v:
Experimental and Molecular Pathology. 70:281-288
The detection of specific RNA molecules in situ is routinely performed using haptenated probes, which are detected by either enzymatic amplification or direct fluorescence. A drawback of fluorescence labeling has been the reduced sensitivity relative
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7132a4f99011d7ea54da651ab00f956
https://doi.org/10.1006/exmp.2001.2365
https://doi.org/10.1006/exmp.2001.2365