Zobrazeno 1 - 10
of 61
pro vyhledávání: '"John G Coote"'
Publikováno v:
PLoS ONE, Vol 8, Iss 8, p e71524 (2013)
A reporter plasmid pSRG has been developed which expresses red fluorescent protein (RFP) from a constitutive prokaryotic promoter within Pasteurella multocida B:2 and green fluorescent protein (GFP) from a constitutive eukaryotic promoter within mamm
Externí odkaz:
https://doaj.org/article/4e61a126c8cb4d3eace056983a7be75a
Publikováno v:
Microbial Pathogenesis. 52:353-358
A Pasteurella multocida B:2 strain from a case of bovine haemorrhagic septicaemia (HS) and a derivative, JRMT12, that was attenuated by a deletion in the aroA gene, were shown to adhere to, invade and survive within cultured embryonic bovine lung (EB
Publikováno v:
Microbial Pathogenesis. 51:169-177
A protein designated Bap-5 (GenBank accession no. AF081494 ) or BapC (GenBank accession no. AJ277634 ) has been identified as a member of the Bordetella pertussis autotransporter family and the present work suggests that this protein, like the previo
Publikováno v:
Veterinary Microbiology. 150:331-337
The objective of this study was to investigate the haemolytic and cytotoxic activity of Pasteurella multocida B:2 strains, originally from cases of haemorrhagic septicaemia in cattle. All six P. multocida B:2 strains were non-haemolytic on sheep bloo
Autor:
Sandra Prior, Gordon Yiu Chong Cheung, Thomas J. Jess, John G. Coote, Roger Parton, Nicholas C. Price, Sharon M. Kelly
Publikováno v:
Microbial Pathogenesis. 46:36-42
A comparison was made of the cytotoxic activity and secondary structural features of four recombinant forms of adenylate cyclase toxin (CyaA). These forms were fully functional CyaA, CyaA lacking adenylate cyclase enzymatic activity (CyaA*), and non-
Autor:
Paul Dickinson, Roger Parton, Gordon Y. C. Cheung, Garwin Sing, Marie Craigon, John G. Coote, Peter Ghazal
Publikováno v:
Microbial Pathogenesis. 44:61-70
Three different recombinant forms of CyaA were used to investigate transcriptional responses of murine bone marrow-derived macrophages (BMMs) using Affymetrix Mouse Genome GeneChips®. These forms were enzymically active, invasive CyaA, non-enzymical
Autor:
J. Christopher Hodgson, Jill Sales, Anna Finucane, Roger Parton, John G. Coote, Jeanie Finlayson, Saeed Ataei, Mark P. Dagleish
Publikováno v:
Infection and Immunity. 75:5837-5844
Three groups of five calves, namely, V1, V2, and V3, were immunized intramuscularly at 4 and 8 weeks of age with ca. 109, 108, and 107CFU, respectively, of a derivative ofPasteurella multocidaB:2 wild-type strain 85020 containing a deletion in thearo
Autor:
Dorothy K.L. Xing, Sheena R. Gomez, Chun-Ting Yuen, John G. Coote, Roger Parton, Michael J. Corbel
Publikováno v:
Analytical Biochemistry. 356:244-253
Pertussis toxin (PTx) is a major virulence factor produced by Bordetella pertussis and, in its detoxified form PTd, is an important component of pertussis vaccines. The in vivo histamine sensitization test (HIST) is currently used for the safety test
Publikováno v:
Journal of Medical Microbiology. 53:355-365
To determine the value of gene markers for surveillance and to assess the genetic stability of potential acellular pertussis vaccine components, the sequence variation in ten virulence-related genes of Bordetella pertussis was investigated in strains
Autor:
Paul D. Lawrence, F. Yasmin Kazi, Mathis O. Riehle, Anthony J. Lawrence, Barbara M. Orr, John Young, Nicholas C. Price, James Sinclair, Julia MacDonald-Fyall, Roger Parton, John G. Coote
Publikováno v:
Journal of Biological Chemistry. 277:22289-22296
Continuous recording of the activity of recombinant adenylate cyclase (CyaA) of Bordetella pertussis (EC 4.6.1.1) by conductimetric determination of enzyme-coupled pyrophosphate cleavage has enabled us to define a number of novel features of the acti