Durable transgene expression and efficient re-administration after rAAV2.5T-mediated fCFTRΔR gene delivery to adult ferret lungs

Autor: Yinghua Tang, Mehrnoosh Ebadi, Junying Lei, Zehua Feng, Shahab Fakhari, Peipei Wu, Mark D. Smith, Maria P. Limberis, Roland Kolbeck, Katherine J. Excoffon, Ziying Yan, John F. Engelhardt

Publikováno v: Molecular Therapy: Methods & Clinical Development, Vol 32, Iss 2, Pp 101244- (2024)

The dosing interval for effective recombinant adeno-associated virus (rAAV)-mediated gene therapy of cystic fibrosis lung disease remains unknown. Here, we assessed the durability of rAAV2.5T-fCFTRΔR-mediated transgene expression and neutralizing an

Externí odkaz: https://doaj.org/article/27a6b1d9b32d4d5fbd5e3361073b18ed

Immunosuppression reduces rAAV2.5T neutralizing antibodies that limit efficacy following repeat dosing to ferret lungs

Autor: Yinghua Tang, Shahab Fakhari, Eric D. Huntemann, Zehua Feng, Peipei Wu, William Y. Feng, Junying Lei, Feng Yuan, Katherine J. Excoffon, Kai Wang, Maria P. Limberis, Roland Kolbeck, Ziying Yan, John F. Engelhardt

Publikováno v: Molecular Therapy: Methods & Clinical Development, Vol 29, Iss , Pp 70-80 (2023)

The efficacy of redosing the recombinant adeno-associated virus (rAAV) vector rAAV2.5T to ferret lung is limited by AAV neutralizing antibody (NAb) responses. While immunosuppression strategies have allowed for systemic rAAV repeat dosing, their util

Externí odkaz: https://doaj.org/article/1cbaa351bc964c2ea2b39626805cbbeb

Development and characterization of ferret ex vivo tracheal injury and cell engraftment model

Autor: Vitaly Ievlev, Albert C. Pai, Drew S. Dillon, Spencer Kuhl, Thomas J. Lynch, Kyle W. Freischlag, Caitlyn B. Gries, John F. Engelhardt, Kalpaj R. Parekh

Publikováno v: Frontiers in Medicine, Vol 10 (2023)

The field of airway biology research relies primarily on in vitro and in vivo models of disease and injury. The use of ex vivo models to study airway injury and cell-based therapies remains largely unexplored although such models have the potential t

Externí odkaz: https://doaj.org/article/93e055f61a2548cc8dc2449b72fa86ca

Extant diversity of bryophytes emerged from successive post-Mesozoic diversification bursts

Autor: Jochen Heinrichs, Blanka Shaw, A. J. Shaw, M von Konrat, E. C. Davis, Stuart F. McDaniel, David G. Long, Endymion D. Cooper, John F. Engel, Harald Schneider, Alain Vanderpoorten, Emmanuel Paradis, Bernard Goffinet, Barbara Crandall-Stotler, Michelle L. Hollingsworth, Benjamin Laenen, Aurélie Désamoré, Cymon J. Cox, Laura L. Forrest, Jairo Patiño, Juan Carlos Villarreal, S. R. Gradstein

Publikováno v: Nature Communications
Nature Communications, Nature Publishing Group, 2014, 5 (1), ⟨10.1038/ncomms6134⟩
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP

Unraveling the macroevolutionary history of bryophytes, which arose soon after the origin of land plants but exhibit substantially lower species richness than the more recently derived angiosperms, has been challenged by the scarce fossil record. Her

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27ef7973297de993be60f44b8f14171c
http://www.documentation.ird.fr/hor/fdi:010062669

Oxidative stress and impaired insulin secretion in cystic fibrosis pig pancreas

Autor: Yunxia O'Malley, Mitchell C. Coleman, Xingshen Sun, Junying Lei, Jianrong Yao, Casey F. Pulliam, Paige Kluz, Michael L. McCormick, Yaling Yi, Yumi Imai, John F. Engelhardt, Andrew W. Norris, Douglas R. Spitz, Aliye Uc

Publikováno v: Advances in Redox Research, Vol 5, Iss , Pp 100040- (2022)

Cystic fibrosis-related diabetes (CFRD) is one the most common comorbidities in cystic fibrosis (CF). Pancreatic oxidative stress has been postulated in the pathogenesis of CFRD, but no studies have been done to show an association. The main obstacle

Externí odkaz: https://doaj.org/article/522cd40a4710415fae812e3ff30a32cd