Výsledky vyhledávání - "John E. McCartney"

Avidity-Mediated Enhancement of In vivo Tumor Targeting by Single-Chain Fv Dimers

Autor: John E. McCartney, James S. Huston, Louis M. Weiner, Mei-Sheng Tai, L. L. Houston, Gregory P. Adams, James D. Marks, Walter F. Stafford

Publikováno v: Clinical Cancer Research. 12:1599-1605

Radiolabeled single-chain Fv (sFv) molecules display highly specific tumor retention in the severe combined immunodeficient (SCID) mouse model; however, the absolute quantity of sFv retained in the tumors is diminished by the rapid renal elimination

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e092d0e4bdc85653ba55ddb1d06ff8b
https://doi.org/10.1158/1078-0432.ccr-05-2217

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Optimization of in vivo tumor targeting in SCID mice with divalent forms of 741F8 anti-c-erbB-2 single-chain Fv: effects of dose escalation and repeated i.v. administration

Autor: Jamie Eisenberg, John E. McCartney, Michael A. Bookman, Gregory P. Adams, E J Wolf, Walter F. Stafford, Mei-Sheng Tai, L. L. Houston, Louis M. Weiner, James S. Huston

Publikováno v: Cancer Immunology, Immunotherapy. 40:299-306

Single-chain Fv molecules in monovalent (sFv) and divalent [(sFv')2] forms exhibit highly specific tumor targeting in mice as a result of their small size and rapid systemic clearance. As a consequence, there is a rapid reversal of the sFv blood/tumo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::902c4531e34f46172e3f31b3a579bc7e
https://doi.org/10.1007/bf01519629

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Radiometal labeling of recombinant proteins by a genetically engineered minimal chelation site: technetium-99m coordination by single-chain Fv antibody fusion proteins through a C-terminal cysteinyl peptide

Autor: F Jamar, M S Tai, Louis M. Weiner, L L Houston, A. M. Peters, Hermann Oppermann, G P Adams, B T Heelan, Andrew J.T. George, John E. McCartney

Publikováno v: Proceedings of the National Academy of Sciences. 92:8358-8362

We describe a method to facilitate radioimaging with technetium-99m (99mTc) by genetic incorporation of a 99mTc chelation site in recombinant single-chain Fv (sFv) antibody proteins. This method relies on fusion of the sFv C terminus with a Gly4Cys p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::931e2f825c690cb439eb086f4b1f9b90
https://doi.org/10.1073/pnas.92.18.8358

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Preclinical Studies and Optimization of Specific Tumor Delivery for 741F8 sFv, an Anti-c-erbB-2 Single-Chain Species

Autor: John E. McCartney, Axel A. Laminet, James S. Huston, Louis M. Werner, Mei-Sheng Tai, Gregory P. Adams, Sen Liu, Josephine Schultz, Jamie Eisenberg, E J Wolf, Gerald Apell, L. L. Houston, Hermann Oppermann, Walter F. Stafford, Bruce J. Giantonio, Michael A. Bookman

Publikováno v: International Journal of Pharmacognosy. 33:75-91

Single-chain Fv fragments (sFv) that bind tumor-associated antigens exhibit highly specific tumor targeting characteristics, based on studies of intravenous sFv administration to immunodeficient mice bearing human tumor xenografts. These features sug

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f14c21278870505fbb9ed0cf1aa4fcca
https://doi.org/10.3109/13880209509067090

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Engineering disulfide-linked single-chain Fv dimers [(sFv')2] with improved solution and targeting properties: anti-digoxin 26–10 (sFv')2 and anti-c-erbB-2 741F8 (sFv')2 made by protein folding and bonded through C-terminal cysteinyl peptides

Autor: Sen Liu, Gregory P. Adams, Mei-Sheng Tai, Hermann Oppermann, Donald Jin, John E. McCartney, James S. Huston, Robert M. Hudziak, Axel A. Laminet, Irwin Fand, Michael A. Bookman, Louis M. Weiner, Walter F. Stafford, L. L. Houston

Publikováno v: "Protein Engineering, Design and Selection". 8:301-314

Single-chain Fv fusions with C-terminal cysteinyl peptides (sFv') have been engineered using model sFv proteins based upon the 26-10 anti-digoxin IgG and 741F8 anti-c-erbB-2 IgG monoclonal antibodies. As part of the 741F8 sFv construction process, th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::cbcb2dd232369614f19186a6586b3647
https://doi.org/10.1093/protein/8.3.301

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Mammalian Cell Expression of Single–Chain Fv (sFv) Antibody Proteins and Their C–terminal Fusions with Interleukin–2 and Other Effector Domains

Autor: James S. Huston, Haimanti Dorai, Mei-Sheng Tai, Hermann Oppermann, Axel A. Laminet, John E. McCartney, L. L. Houston, Robert M. Hudziak

Publikováno v: Nature Biotechnology. 12:890-897

The production of several single-chain Fv (sFv) antibody proteins was examined by three modes of mammalian cell expression. Our primary model was the 741F8 anti-c-erbB-2 sFv, assembled as either the VH-VL or VL-VH, and expressed alone, with C-termina

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7a79aaa738d4826579e18bc3608925b
https://doi.org/10.1038/nbt0994-890

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Biosynthetic antibody binding sites: Development of a single-chain Fv model based on antidinitrophenol IgA myeloma MOPC 315

Autor: John E. McCartney, Lynne Lederman, Gay-May Wu, James S. Huston, Roberta S. Batorsky, Hermann Oppermann, Eric A. Drier, Nancy A. Cabral-Denison

Publikováno v: Journal of Protein Chemistry. 10:669-683

The functional antigen binding region of antidinitrophenol mouse IgA myeloma MOPC 315 has been produced as a single-chain Fv (sFv) protein in E. coli. Recombinant 315 proteins included sFv alone, a bifunctional fusion protein with amino-terminal frag

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd0bf71f47a7b5c9e4c6d540438a124d
https://doi.org/10.1007/bf01025718

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In vitro and in vivo characterization of a human anti-c-erbB-2 single-chain Fv isolated from a filamentous phage antibody library

Autor: Michael A. Bookman, Robert Schier, John E. McCartney, E J Wolf, L L Houston, Gerald Apell, C Wong, Louis M. Weiner, James S. Huston, James D. Marks

Publikováno v: Immunotechnology : an international journal of immunological engineering. 1(1)

Background: Antibody-based reagents have failed to live up to their anticipated role as highly specific targeting agents for cancer therapy. Targeting with human single-chain Fv (sFv) molecules may overcome some of the limitations of murine IgG, but

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60ee6b6d6b5c92f815cb0be41ce85da5
https://pubmed.ncbi.nlm.nih.gov/9373335

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Tumor targeting in a murine tumor xenograft model with the (sFv')2 divalent form of anti-c-erbB-2 single-chain Fv

Autor: James S. Huston, Gregory P. Adams, John E. McCartney, Mei-Sheng Tai, Robert M. Hudziak, Hermann Oppermann, Walter F. Stafford, Sen Liu, Irwin Fand, Gerald Apell, Axel Laminet, Michael A. Bookman, L. L. Houston, Louis M. Weiner

Publikováno v: Cell biophysics.

This investigation has utilized novel forms of the single-chain Fv (sFv), wherein a cysteine-containing peptide has been fused to the sFv carboxyl terminus to facilitate disulfide bonding or specific cross-linking of this sFv' to make divalent (sFv')

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1182b4aa79de85594efb6e2275abef45
https://pubmed.ncbi.nlm.nih.gov/7736532

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