Zobrazeno 1 - 10
of 94
pro vyhledávání: '"John D. Termine"'
Autor:
John D. Termine, Pamela Gehron Robey
Publikováno v:
Calcified tissue international. 37(5)
Human bone cell cultures were established by maintaining collagenase-treated, bone fragments in low Ca++ medium. The resulting cell cultures exhibited a high level of alkaline phosphatase activity and produced a significant increase in intracellular
Autor:
Pedro Domínguez, Pamela Gehron Robey, John D. Termine, Marian F. Young, Kyomi Ibaraki, Theresa E. Hefferan
Publikováno v:
Journal of Bone and Mineral Research. 6:1127-1136
The cis-acting regulatory elements of the osteonectin gene have been studied using a chloramphenicol acetyltransferase (CAT) promoter assay in osteonectin-expressing and nonexpressing cultured cells. When various stretches of the promoter were transi
Publikováno v:
Journal of Bone and Mineral Research. 7:743-754
In the accompanying study, we report an in vitro culture system from bovine bone cells that can be applied to investigate bone cell growth and differentiation. In this system, bovine bone cells placed in mineralization medium formed multilayers (days
Autor:
Ulrich Vetter, Larry W. Fisher, Walther Vogel, Pamela Gehron Robey, N S Fedarko, Doris Wöhrle, Claudia Geerkens, Walter Just, John D. Termine, Marian F. Young
Publikováno v:
Human Genetics. 96:44-52
We report the mRNA and protein expression levels of human biglycan (BGN) in patients with different numbers of sex chromosomes. BGN maps to the distal long arm of the X chromosome, band Xq28, near the second pseudoautosomal region. BGN expression lev
Publikováno v:
Journal of Histochemistry & Cytochemistry. 41:183-191
We defined two distinct maturational compartments (proliferative and secretory) of osteogenic cells in vivo on the basis of ALP activity, BrdU incorporation, cell shape, and BSP production. BSP immunoreactivity was found to mark cells in the secretor
Autor:
Mara Riminucci, Paolo Bianco, Larry W. Fisher, Ermanno Bonucci, John D. Termine, Pamela Gehron Robey, G. Silvestrini
Publikováno v:
Journal of Histochemistry & Cytochemistry. 41:193-203
Bone sialoprotein (BSP), a bone matrix-enriched glycoprotein containing the Arg-Gly-Asp (RGD) motif and endowed with cell binding properties, was localized in osteoblasts and early bone matrix of developing rat bone at the ultrastructural level. Prel
Autor:
W Vogel, Larry W. Fisher, W Just, Anne-Marie Heegaard, John D. Termine, Ulrich Vetter, M F Young
Publikováno v:
Journal of Biological Chemistry. 266:14371-14377
Biglycan (PG-I, DS-PG-1, PG-S1) is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin (PG-II, PG-S2, DS-PG2, or PG-40) and fibromodulin. The core protein is made up pred
Autor:
Larry W. Fisher, Jerome S. Perlish, Lloydstone Jacobs, Raul Fleischmajer, E. Douglas MacDonald, John D. Termine
Publikováno v:
Journal of Structural Biology. 106:82-90
Biglycan (PG-I, BGN) and decorin (PG-II, DCN) are small proteoglycans that have been isolated in cartilage, skin, and bone. Although the function of biglycan is unknown, there is biochemical evidence that decorin interacts with fibrillar collagens (t
Autor:
Wendi Ding, Carolyn W. Gibson, John D. Termine, Ellis E. Golub, Joel Rosenbloom, M F Young, Hitoyata Shimokawa
Publikováno v:
Biochemical and Biophysical Research Communications. 174:1306-1312
The polymerase chain reaction was used to amplify bovine tooth amelogenin cDNA, resulting in several products which were separated by agarose gel electrophoresis. Sequence determination of one of the products revealed that it encoded an amino acid se
Publikováno v:
Analytical Biochemistry. 188:398-407
Four proteoglycans and hyaluronan synthesized by cultured human bone cells were isolated using a two-step high-performance liquid chromatography system involving desalting and buffer exchange with a TSK-GEL HW 40(S) column followed by ion-exchange se