Zobrazeno 1 - 10
of 153
pro vyhledávání: '"John D Biggers"'
Publikováno v:
PLoS ONE, Vol 9, Iss 6, p e99809 (2014)
The purpose of this study is to develop a mouse sperm preservation method based on evaporative drying. Mouse sperm were evaporatively dried and stored at 4°C and ambient temperature for 3 months to 2 years. Upon rehydration, a single sperm was injec
Externí odkaz:
https://doaj.org/article/d7fe4e1f335847248b103366c065b6ea
Publikováno v:
PLoS ONE, Vol 7, Iss 8, p e42267 (2012)
The prevalence of genetically engineered mice in medical research has led to ever increasing storage costs. Trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following partial desiccation and stora
Externí odkaz:
https://doaj.org/article/8a939c1815ad4c6a85f48d1911b2f9bf
Publikováno v:
PLoS ONE, Vol 7, Iss 1, p e29924 (2012)
With the fast advancement in the genetics and bio-medical fields, the vast number of valuable transgenic and rare genetic mouse models need to be preserved. Preservation of mouse sperm by convective drying and subsequent storing at above freezing tem
Externí odkaz:
https://doaj.org/article/c58afff4d3c744fcbd972cc0a318a482
Publikováno v:
Cryobiology. 98
Vitrification of mammalian oocytes and embryos is typically a two-step procedure involving two solutions of increasing concentrations of cryoprotectants. In the present study, we report a simple vitrification protocol that uses low cryoprotectant con
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc150facf42a9b1cc7037f91fcf13e5c
https://pubmed.ncbi.nlm.nih.gov/33137307
https://pubmed.ncbi.nlm.nih.gov/33137307
Autor:
Mehmet Toner, Diane L. Wright, Gloria Y. Lee, Jie Liu, Thomas L. Toth, Cigdem Tanrikut, John D. Biggers
Publikováno v:
Cryobiology. 73:162-167
Cryopreservation of human spermatozoa is a commonly used technique in assisted reproduction, however freezing low concentrations of sperm while maintaining adequate post-thaw motility remains a challenge. In an effort to optimize post-thaw motility y
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c2a070ac313c97f556e0e0cd692a151
https://doi.org/10.1016/j.cryobiol.2016.08.004
https://doi.org/10.1016/j.cryobiol.2016.08.004
Autor:
John D. Biggers, Michael C. Summers, Fatima Mirzazadeh Mirzai, Sophie Bird, Alan R. Thornhill
Publikováno v:
Human Fertility. 16:278-285
A comparison was made of the development of human zygotes in either a one-step (Global® medium) or two-step culture system (Quinn's Advantage®). A total of 257 normally fertilized 2PN zygotes from 28 patients were used in the study. The study was b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::efc855c6fd1d044b8ff82cd8c3e59194
https://doi.org/10.3109/14647273.2013.806823
https://doi.org/10.3109/14647273.2013.806823
Publikováno v:
Journal of Animal Science. 90:3739-3742
A piezo-driven pipette that includes a small amount of mercury to enhance efficiency is widely used for mouse intracytoplasmic sperm injection (ICSI). Unfortunately, the use of toxic mercury is not permitted in hospital facilities and alternatives to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::508dff9c78b1bd467b61a68ca069fbc4
https://doi.org/10.2527/jas.2012-5136
https://doi.org/10.2527/jas.2012-5136
Autor:
Kimball O. Pomeroy, William E. Gibbons, John D. Biggers, Barry Behr, Judy E. Stern, Catherine Racowsky
Publikováno v:
Fertility and Sterility. 95:1985-1989
Objective To evaluate the validity of collecting day 3 embryo morphology variables into the Society for Assisted Reproductive Technology Clinic Outcomes Reporting System (SART CORS). Design Retrospective. Setting National database—SART CORS. Patien
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72d81eb00e9c10e5878e2f83ae01cb27
https://doi.org/10.1016/j.fertnstert.2011.02.009
https://doi.org/10.1016/j.fertnstert.2011.02.009
Publikováno v:
Human Reproduction. 24:2104-2113
This study was undertaken to determine what characteristics should be recorded on which days to build a predictive model for selection of Day 3 embryos.Embryos failing to form a clinical sac or that formed a viable fetus (toor =12 weeks), and transfe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b2f8d20a51827c2362b3ac2caadbbb0
https://doi.org/10.1093/humrep/dep198
https://doi.org/10.1093/humrep/dep198