Zobrazeno 1 - 10
of 67
pro vyhledávání: '"John C. Owicki"'
Publikováno v:
Journal of Fluorescence. 15:363-375
As the sophistication of instruments that make fluorimetric measurements on samples in microplates has increased, so has the need for methods to validate instrumental performance. This paper describes a solid-state validation microplate that tests mu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4430bcaef0a308b908e7070a37692a5b
https://doi.org/10.1007/s10895-005-2631-2
https://doi.org/10.1007/s10895-005-2631-2
Autor:
John C. Owicki
Publikováno v:
Acta Histochemica. 100:339-343
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::68e3997144304bb2e1415ee84479a67d
https://doi.org/10.1016/s0065-1281(98)80031-7
https://doi.org/10.1016/s0065-1281(98)80031-7
Publikováno v:
Sensors and Actuators A: Physical. 63:47-57
The surface potential of semiconductor devices in contact with electrolyte solutions is an important part of signal transduction for a variety of bioanalytical devices. Here we have investigated the lateral resolution at which the surface potential m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::17bb65f0fc2e0167fb9b360bc246b8ba
https://doi.org/10.1016/s0924-4247(97)80428-1
https://doi.org/10.1016/s0924-4247(97)80428-1
Publikováno v:
Biophysical Journal. 64(3):905-918
We studied specific membrane-membrane interactions mediated by ligand-receptor binding in a model system, which consisted of (a) FG3P, the fluorescein hapten attached to a phospholipid by a peptidyl spacer as described previously (Petrossian, A., A.B
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1432ba4c64f45d8dfa3cc4b7b039804d
Autor:
S Pitchford, Harden M. McConnell, J. W. Parce, D.L. Miller, John C. Owicki, G T Baxter, H G Wada
Publikováno v:
Science. 257:1906-1912
A silicon-based device, dubbed a microphysiometer, can be used to detect and monitor the response of cells to a variety of chemical substances, especially ligands for specific plasma membrane receptors. The microphysiometer measures the rate of proto
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5787fe38a35b49e881df0865de298cbc
https://doi.org/10.1126/science.1329199
https://doi.org/10.1126/science.1329199
Publikováno v:
Current Opinion in Structural Biology. 1:647-652
Biosensors provide analytical information about biochemical and biological systems. One such device is a light-addressable potentiometric sensor at the heart of an instrument called a microphysiometer. The instrument detects a signal that is closely
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::34886b6fcfc9b4962bf0ebd6d716478b
https://doi.org/10.1016/s0959-440x(05)80091-0
https://doi.org/10.1016/s0959-440x(05)80091-0
Publikováno v:
Micro Total Analysis Systems 2002 ISBN: 9789401039536
A new method of detecting changes in the electrophoretic mobility of fluorescent molecules in a microfluidic device suitable for high throughput experimentation is presented. This approach is based on applying a pulsed electric field to modulate the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::500e547d86f0be32112242c2306bc3fd
https://doi.org/10.1007/978-94-010-0504-3_19
https://doi.org/10.1007/978-94-010-0504-3_19
Autor:
John C. Owicki
Publikováno v:
Journal of biomolecular screening. 5(5)
Fluorescence polarization and anisotropy are two nearly equivalent techniques that have together, over the past 5 years, achieved wide use in high throughput screening in drug discovery. These are single-label methods that can be used to construct ho
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f58e773a1afb583ae96e7a5028934b8
https://pubmed.ncbi.nlm.nih.gov/11080688
https://pubmed.ncbi.nlm.nih.gov/11080688
Publikováno v:
SPIE Proceedings.
A fluorescence polarization assay was developed as an alternative to the radiolabeled SPA assays currently used to monitor the activity of tyrosine kinases in drug discovery. The assay can be used with enzymes having substrate specificity similar to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0c96ff30406e037bb6460ea21c96629d
https://doi.org/10.1117/12.346748
https://doi.org/10.1117/12.346748
Autor:
Douglas N. Modlin, Will Burton, Kimberly Crawford, Sudhir S. Deshpande, I. Mineyev, John C. Owicki, Todd E. French
Publikováno v:
SPIE Proceedings.
Fluorescence lifetime, the mean interval between absorption and emission, is as fundamental a characteristic of fluorescence as excitation and emission wavelengths and quantum yield. Yet, with the exception of time-resolved fluorescence assays utiliz
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1c7b1337f9e304cbb7a6708cf04b43d9
https://doi.org/10.1117/12.307330
https://doi.org/10.1117/12.307330