Zobrazeno 1 - 10
of 70
pro vyhledávání: '"John C. Gebler"'
Autor:
Ying Qing Yu, Pauline M. Rudd, William R. Alley, John C. Gebler, Mark Hilliard, Ciara A. McManus, Sinead Hallinan
Publikováno v:
mAbs
Glycosylation is an important attribute of biopharmaceutical products to monitor from development through production. However, glycosylation analysis has traditionally been a time-consuming process with long sample preparation protocols and manual in
Publikováno v:
Analytical Chemistry. 81:3485-3499
Current chromatographic and mass spectrometric techniques have limitations for analyzing heparin and heparin oligomers due to their high polarity, structural diversity, and sulfate lability. A rapid method for the analysis of heparin oligosaccharides
Autor:
Aleksander Jaworski, Asish B. Chakraborty, Martin Gilar, John C. Gebler, Scott J. Geromanos, Petra Olivova
Publikováno v:
ELECTROPHORESIS. 30:1157-1167
1-D and 2-D LC methods were utilized for proteome analysis of undepleted human serum. Separation of peptides in 2-D LC was performed either with strong cation exchange (SCX)-RP chromatography or with an RP-RP 2-D LC approach. Peptides were identified
Publikováno v:
Journal of Chromatography A. 1191:162-170
A mixed-mode chromatographic (MMC) sorbent was prepared by functionalizing the silica sorbent with a pentafluorophenyl (PFP) ligand. The resulting stationary phase provided a reversed-phase (RP) retention mode along with a relatively mild strong cati
Publikováno v:
Rapid Communications in Mass Spectrometry. 22:29-40
This paper presents an improved analytical method for glycosylation structural characterizations of a monoclonal antibody (mAb) using a newly developed quadrupole ion-mobility time-of-flight (ESI-Q-IM-TOF) mass spectrometer. Using this method, high-r
Publikováno v:
Rapid Communications in Mass Spectrometry. 21:2813-2821
A retention prediction model was developed for peptides separated in reversed-phase chromatography. The model was utilized to identify and exclude the false positive (FP) peptide identifications obtained via database search. The selected database inc
Autor:
Michael J. Lavallee, John C. Gebler, Robert A. Collamati, Jeffrey W. Finch, Hongji Liu, Christopher C. Benevides
Publikováno v:
Journal of Chromatography A. 1147:30-36
The effects of the column length, the particle size, the gradient length and the flow rate of a nanoLC system on peptide peak capacity were investigated and compared. Columns packed with 1.7 microm and 3 microm C(18) materials into pieces of 75 micro
Publikováno v:
Rapid Communications in Mass Spectrometry. 21:730-744
Liquid chromatography/mass spectrometry (LC/MS) peptide maps have become a basic tool for characterizing proteins of biological and pharmaceutical interest. The ability to generate reproducible maps with high protein sequence coverage is a central go
Publikováno v:
Analytical Chemistry. 79:1731-1738
Glycopeptides are typically prepared by cleaving the proteins with specific proteolytic enzymes, such as trypsin. The resulting glycopeptides tend to have weak mass spectrometry ion signals (ESI or MALDI) due to their relatively large molecular weigh
Publikováno v:
Journal of Proteome Research. 5:135-146
In this paper, we present a combined top-down/bottom-up proteomic analysis workflow for the characterization of proteomic samples. This workflow combines protein fractionation (multidimensional chromatographic separation) with parallel online ESI-TOF