Zobrazeno 1 - 10
of 126
pro vyhledávání: '"John C. Chaput"'
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-11 (2023)
Abstract Low activity has been the primary obstacle impeding the use of DNA enzymes (DNAzymes) as gene silencing agents in clinical applications. Here we describe the chemical evolution of a DNAzyme with strong catalytic activity under near physiolog
Externí odkaz:
https://doaj.org/article/90e6e03d316f4a4193a3570f10b8462e
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-8 (2021)
DNA polymerases are the key enzymes responsible for DNA replication and repair. Here the authors reveal through time-lapsed images of X-ray crystal structures that translocation precedes phosphodiester bond formation in the mechanism of DNA synthesis
Externí odkaz:
https://doaj.org/article/fdef6c3a75d9466da9d07b297cab93f2
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
Artificial genetic polymers (XNAs) have been explored for their nuclease activity, but XNAzymes have proven challenging to discover by in vitro selection. Here, the authors generate an efficient RNA-cleaving 2’-fluoroarabino nucleic acid enzyme (FA
Externí odkaz:
https://doaj.org/article/596b420be50f478c8b1343b5a6191768
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
The laboratory-evolved polymerase Kod-RI catalyzes α-L-threose nucleic acid (TNA) synthesis. Here, the authors present Kod-RI crystal structures that give insights into how TNA triphosphates are selected and extended in a template-dependent manner,
Externí odkaz:
https://doaj.org/article/3bbeaf8d41a942a9b6a734d7962c9baa
Autor:
Andrew C. Larsen, Matthew R. Dunn, Andrew Hatch, Sujay P. Sau, Cody Youngbull, John C. Chaput
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-9 (2016)
Droplet-based optical polymerase sorting employs a fluorescent sensor to monitor polymerase activity inside the microenvironment of uniform water-in-oil emulsions. Here, the authors use this technique to select and isolate single cells for evolution
Externí odkaz:
https://doaj.org/article/c7f8937b5925459eaf6e4d4fd43af4f8
Autor:
Li Zhang, John C. Chaput
Publikováno v:
Molecules, Vol 25, Iss 18, p 4194 (2020)
Recent advances in polymerase engineering have made it possible to isolate aptamers from libraries of synthetic genetic polymers (XNAs) with backbone structures that are distinct from those found in nature. However, nearly all of the XNA aptamers pro
Externí odkaz:
https://doaj.org/article/c1b1a142b9c7451f87e7ed77d999daf1
Publikováno v:
BioTechniques, Vol 59, Iss 3, Pp 149-151 (2015)
Cell-free protein synthesis (CFPS) allows researchers to rapidly generate functional proteins independent of cell culture. Although advances in eukaryotic lysates have increased the amount of protein that can be produced, the nuances of different tra
Externí odkaz:
https://doaj.org/article/0919a52293bc4a38af8c719ab22af468
Publikováno v:
Nucleic Acids Research. 50:9663-9674
Xeno-nucleic acids (XNAs) are synthetic genetic polymers with backbone structures composed of non-ribose or non-deoxyribose sugars. Phosphonomethylthreosyl nucleic acid (pTNA), a type of XNA that does not base pair with DNA or RNA, has been suggested
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80060592821b19a05edaa15ee793c65f
https://doi.org/10.1093/nar/gkac792
https://doi.org/10.1093/nar/gkac792
Autor:
Robert C. Spitale, John C. Chaput
Publikováno v:
Nature Chemistry. 14:859-861
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::955754110bdec77d80d42f9bb7831dfc
https://doi.org/10.1038/s41557-022-00983-4
https://doi.org/10.1038/s41557-022-00983-4