Zobrazeno 1 - 10
of 10
pro vyhledávání: '"John C. Bramley"'
Autor:
Alex L. Yenkin, John C. Bramley, Colin L. Kremitzki, Jason E. Waligorski, Mariel J. Liebeskind, Xinyuan E. Xu, Vinay D. Chandrasekaran, Maria A. Vakaki, Graham W. Bachman, Robi D. Mitra, Jeffrey D. Milbrandt, William J. Buchser
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-14 (2022)
Raft-Seq is a generalizable pooled screening platform that combines high-content imaging, machine learning and microraft isolation, and enables efficient screening of genetic perturbations based on their impact on phenotypes.
Externí odkaz:
https://doaj.org/article/63108157402f4421b5e89ea34d7f7eab
Autor:
John C. Bramley, Coyne G. Drummond, Nicholas J. Lennemann, Charles A. Good, Kwang Sik Kim, Carolyn B. Coyne
Publikováno v:
mSphere, Vol 2, Iss 3 (2017)
ABSTRACT The blood-brain barrier (BBB) comprises the foremost protective barrier in the brain and is composed in part of a layer of microvascular endothelial cells that line the capillaries surrounding the brain. Here, we describe a human three-dimen
Externí odkaz:
https://doaj.org/article/d4742a1ff0b441fa9ea71cda5c0ec1a3
Autor:
William Buchser, Aaron DiAntonio, John C. Bramley, Alex L. Yenkin, Jeffrey Milbrandt, Mark A. Zaydman
Publikováno v:
Scientific Data, Vol 7, Iss 1, Pp 1-11 (2020)
Scientific Data
Scientific Data
Protein domain-based approaches to analyzing sequence data are valuable tools for examining and exploring genomic architecture across genomes of different organisms. Here, we present a complete dataset of domains from the publicly available sequence
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43b33295d4a717483460e778a6d5d75c
http://link.springer.com/article/10.1038/s41597-020-0536-1
http://link.springer.com/article/10.1038/s41597-020-0536-1
Autor:
John C. Bramley, Marianna Vakaki, William Buchser, Jason Waligorski, Robi D. Mitra, Alex L. Yenkin, Mariel J. Liebeskind, Xiaoxiao Xu, Chandrasekaran Vd, Colin Kremitzki, Jeffrey Milbrandt
Most human genetic variation is classified as VUS - variants of uncertain significance. While advances in genome editing have allowed innovation in pooled screening platforms, many screens deal with relatively simple readouts (viability, fluorescence
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8cd0b9f59d3e6141461d898e89e71f6e
https://doi.org/10.1101/2021.03.12.434746
https://doi.org/10.1101/2021.03.12.434746
Autor:
Jeffrey Milbrandt, Matthew A. Lalli, William Buchser, Stacey L. House, Mariel J. Liebeskind, Philip A. Mudd, Alex L. Yenkin, Colin Kremitzki, John C. Bramley, Robi D. Mitra, Jane A. O’Halloran, Xinyuan E Xu, Jason Waligorski
Distributed “Point-of-Care” or “at-Home” testing is an important component for a complete suite of testing solutions. This manuscript describes the construction and operation of a platform technology designed to meet this need. The ongoing CO
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e7d6498a6d4d731d7e02ca64e1e68b53
https://doi.org/10.1101/2020.10.19.20215319
https://doi.org/10.1101/2020.10.19.20215319
Autor:
Natalia S. Harasymowicz, Chia-Lung Wu, Ruhang Tang, Neda Rashidi, Alireza Savadipour, William Buchser, John C. Bramley, Farshid Guilak
Publikováno v:
FASEB J
Macrophages and other immune cells are important contributors to obesity-associated inflammation; however, the cellular identities of these specific populations remain unknown. In this study, we identified individual populations of myeloid cells foun
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17e84986762df292c9b75afbc6f7e422
https://pubmed.ncbi.nlm.nih.gov/33566380
https://pubmed.ncbi.nlm.nih.gov/33566380
Publikováno v:
Methods in Molecular Biology ISBN: 9781071605844
A network of intersecting molecular pathways interacts to initiate and execute axon destruction. Maximum protection against axon degeneration likely requires more than manipulation of a single target. Here, we describe the process of designing a high
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3ab4d4360b8aae55c09d80885b44c5f5
https://doi.org/10.1007/978-1-0716-0585-1_6
https://doi.org/10.1007/978-1-0716-0585-1_6
Autor:
Coyne G. Drummond, Kwang Sik Kim, Charles A. Good, Nicholas J. Lennemann, John C. Bramley, Carolyn B. Coyne
Publikováno v:
mSphere, Vol 2, Iss 3, p e00206-17 (2017)
mSphere
mSphere, Vol 2, Iss 3 (2017)
mSphere
mSphere, Vol 2, Iss 3 (2017)
Neurotropic viral infections are significant sources of global morbidity and mortality. The blood-brain barrier (BBB) is composed in part of a layer of microvascular endothelial cells and functions to restrict viral access to the brain. In vitro mode
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2e646368d339e038ce3aa3004c3cbd0
https://doaj.org/article/3e611312612d4adaa8c3194786fd97d7
https://doaj.org/article/3e611312612d4adaa8c3194786fd97d7
Autor:
Avraham Bayer, Carolyn B. Coyne, Ernesto Torres De Azeved Marques, Yoel Sadovsky, Stefanie A. Morosky, Nicholas J. Lennemann, John C. Bramley, Sara Cherry, Yingshi Ouyang
During mammalian pregnancy, the placenta acts as a barrier between the maternal and fetal compartments. The recently observed association between Zika virus (ZIKV) infection during human pregnancy and fetal microcephaly and other anomalies suggests t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b2d82d69db5d775d35dc16dac3cb963a
https://europepmc.org/articles/PMC4866896/
https://europepmc.org/articles/PMC4866896/
Publikováno v:
Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology. 202(11)
The integrity of long axons is essential for neural communication. Unfortunately, relatively minor stress to a neuron can cause extensive loss of this integrity. Axon degeneration is the cell-intrinsic program that actively deconstructs an axon after
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c5f52d1c25a3e28fded3f9c0d1657d7
https://pubmed.ncbi.nlm.nih.gov/27614771
https://pubmed.ncbi.nlm.nih.gov/27614771