Výsledky vyhledávání - "John B. Sharefkin"

Heparin and dibutyryl cAMP modulate gene expression in stimulated human saphenous vein smooth muscle cells

Autor: Hiroyuki Watanabe, Wendy Baur, Harald Gallati, Scott L. Diamond, John B. Sharefkin, Masahiko Yamaguchi

Publikováno v: In Vitro Cellular & Developmental Biology - Animal. 29:867-872

Increased expression of basic fibroblast growth factor (bFGF), platelet-derived growth factor (PDGF) A chain, and tissue plasminogen activator (tPA) by smooth muscle cells (SMC) has been postulated to mediate the progression of intimal hyperplasia. W

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d204dc06bbc6396c58ea6fb52c4626d
https://doi.org/10.1007/bf02631365

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Enzymatic harvesting of adult human saphenous vein endothelial cells: Use of a chemically defined combination of two purified enzymes to attain viable cell yields equal to those attained by crude bacterial collagenase preparations

Autor: William Suggs, Harold Van Wart, John B. Sharefkin

Publikováno v: Journal of Vascular Surgery. 15:0205-0213

Seeding vascular prostheses with enzymatically harvested endothelial cells can create endothelial linings that improve small-caliber prosthetic patency. But crude bacterial collagenases used for endothelial harvest contain cytotoxic nonspecific prote

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea014057d439d92b5f273260f172c103
https://doi.org/10.1067/mva.1992.30863

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Evaluation of the Role of Shiga and Shiga-like Toxins in Mediating Direct Damage to Human Vascular Endothelial Cells

Autor: Vernon L. Tesh, L P Perera, Alison D. O'Brien, James E. Samuel, John B. Sharefkin

Publikováno v: Journal of Infectious Diseases. 164:344-352

Infection with Shiga toxin- and Shiga-like toxin-producing strains of Shigella dysenteriae and Escherichia coli, respectively, can progress to the hemolytic-uremic syndrome. It has been hypothesized that circulating Shiga toxin, Shiga-like toxins, an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa6464f64f5af7e5c26e2be97a774430
https://doi.org/10.1093/infdis/164.2.344

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Fluid flow decreases preproendothelin mRNA levels and suppresses endothelin-1 peptide release in cultured human endothelial cells

Autor: John B. Sharefkin, Carl W. Dieffenbach, Suzanne G. Eskin, Larry V. McIntire, Scott L. Diamond

Publikováno v: Journal of Vascular Surgery. 14:1-9

Endothelin-1, a 21-amino acid peptide secreted by endothelial cells, has constrictor and mitogenic activity for vascular smooth muscle cells, and its mitogenic activity is synergistic with that of platelet-derived growth factor. Endothelial cell—de

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3067e3e12b82cf166470bf10459781cf
https://doi.org/10.1067/mva.1991.27207

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Recombinant DNA and Surgery

Autor: John B. Sharefkin, James M. Brown, Alden H. Harken

Publikováno v: Annals of Surgery. 212:178-186

We have the ability to isolate DNA from tissue, determine its base-pair sequence, and ask if a gene of interest is present. DNA strands can be isolated from one type of cell or organism, cleaved, and inserted (recombined) with DNA from another cell o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d32ec9da65874f5f5ed467e9471124c5
https://doi.org/10.1097/00000658-199008000-00011

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Tissue plasminogen activator messenger RNA levels increase in cultured human endothelial cells exposed to laminar shear stress

Autor: John B. Sharefkin, Larry V. McIntire, S. G. Eskin, K. Frasier-Scott, Carl W. Dieffenbach, Scott L. Diamond

Publikováno v: Journal of Cellular Physiology. 143:364-371

Fluid shear stress can stimulate secretion of tissue plasminogen activator (tPA) by cultured human endothelial cells, while plasminogen activator inhibitor type-1 secretion remains unstimulated. To determine whether hemodynamically induced changes in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d4a466a723f370327197d40581eeb0d
https://doi.org/10.1002/jcp.1041430222

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A computer program for selection of oligonucleotide primers for polymerase chain reactions

Autor: Shi Qi Yang, John B. Sharefkin, Carl W. Dieffenbach, Todd M. Lowe

Publikováno v: Nucleic Acids Research. 18:1757-1761

We have designed a computer program which rapidly scans nucleic acid sequences to select all possible pairs of oligonucleotides suitable for use as primers to direct efficient DNA amplification by the polymerase chain reaction. This program is based

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a3872e259e284e9d4328376b60bf08d
https://doi.org/10.1093/nar/18.7.1757

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