Zobrazeno 1 - 10
of 28
pro vyhledávání: '"John B. March"'
Publikováno v:
BioTechniques, Vol 29, Iss 3, Pp 536-542 (2000)
The stability of restriction enzymes as supplied by manufacturers without any modification has been examined. No reduction in activity was observed for three enzymes (HindIII, EcoRI and Tsp509I) held at ambient temperature or 4°C for the period of s
Externí odkaz:
https://doaj.org/article/77a398f11ef64b61b42ab025dae1513b
Publikováno v:
Veterinary Immunology and Immunopathology. 144:389-395
A bacteriophage-delivered DNA vaccine against Chlamydophila abortus was constructed by cloning a eukaryotic cassette containing the ompA gene (which expresses the Major Outer Membrane Protein) into a bacteriophage lambda vector. Four groups, each of
Publikováno v:
FEMS Immunology & Medical Microbiology. 61:197-204
A bacteriophage lambda DNA vaccine expressing the small surface antigen (HBsAg) of hepatitis B was compared with Engerix B, a commercially available vaccine based on the homologous recombinant protein (r-HBsAg). Rabbits (five per group) were vaccinat
Autor:
John B. March
Publikováno v:
Expert Review of Vaccines. 5:753-759
Autor:
Jason R. Clark, John B. March
Publikováno v:
Trends in Biotechnology. 24:212-218
In recent years it has been recognized that bacteriophages have several potential applications in the modern biotechnology industry: they have been proposed as delivery vehicles for protein and DNA vaccines; as gene therapy delivery vehicles; as alte
Publikováno v:
Infection and Immunity. 74:167-174
A new strategy for rapidly selecting and testing genetic vaccines has been developed, in which a whole genome library is cloned into a bacteriophage λ ZAP Express vector which contains both prokaryotic (P lac ) and eukaryotic (P CMV ) promoters upst
Autor:
John B. March
Publikováno v:
Vaccine. 22:4358-4364
Contagious bovine pleuropneumonia (CBPP) is an economically important transboundary disease, widely present in sub-saharan Africa. Social, cultural and economic factors mean that effective vaccination is the only viable control policy at present. Unf
Autor:
Jason R. Clark, John B. March
Publikováno v:
Expert Review of Vaccines. 3:463-476
Bacteriophages (or phages) are viruses of bacteria, consisting of nucleic acid packaged within a protein coat. In eukaryotic hosts, phages are unable to replicate and in the absence of a suitable prokaryotic host, behave as inert particulate antigens
Publikováno v:
Vaccine. 22:1666-1671
Mice and rabbits have been vaccinated with whole bacteriophage lambda particles containing a DNA vaccine expression cassette under the control of the CMV promoter (enhanced green fluorescent protein [lambda-EGFP] or hepatitis B surface antigen [lambd
Autor:
John B. March
Publikováno v:
Expert Review of Vaccines. 3:113-117