Correlative light and electron microscopy suggests that mutant huntingtin dysregulates the endolysosomal pathway in presymptomatic Huntington’s disease

Autor: Ya Zhou, Thomas R. Peskett, Christian Landles, John B. Warner, Kirupa Sathasivam, Edward J. Smith, Shu Chen, Ronald Wetzel, Hilal A. Lashuel, Gillian P. Bates, Helen R. Saibil

Publikováno v: Acta Neuropathologica Communications, Vol 9, Iss 1, Pp 1-21 (2021)

Abstract Huntington’s disease (HD) is a late onset, inherited neurodegenerative disorder for which early pathogenic events remain poorly understood. Here we show that mutant exon 1 HTT proteins are recruited to a subset of cytoplasmic aggregates in

Externí odkaz: https://doaj.org/article/0cbf701f6cb54600ae9ad669c9f4899d

Correlative light and electron microscopy suggests that mutant huntingtin dysregulates the endolysosomal pathway in presymptomatic Huntington's disease

Autor: Thomas R. Peskett, John B. Warner, Hilal A. Lashuel, Shu Chen, Ya Zhou, Gillian P. Bates, Ronald Wetzel, Kirupa Sathasivam, Edward J Smith, Helen R. Saibil, Christian Landles

Publikováno v: Acta Neuropathologica Communications
Acta Neuropathologica Communications, Vol 9, Iss 1, Pp 1-21 (2021)
Acta Neuropathologica Communications, 9 (1)

Huntington’s disease (HD) is a late onset, inherited neurodegenerative disorder for which early pathogenic events remain poorly understood. Here we show that mutant exon 1 HTT proteins are recruited to a subset of cytoplasmic aggregates in the cell

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fb45d96ccfe575fa106e7eb01cb9ad0
https://pubmed.ncbi.nlm.nih.gov/33853668

Real-Time In Situ Secondary Structure Analysis of Protein Monolayer with Mid-Infrared Plasmonic Nanoantennas

Autor: Dordaneh Etezadi, Hatice Altug, John B. Warner, Hilal A. Lashuel

Publikováno v: ACS Sensors

Dynamic detection of protein conformational changes at physiological conditions on a minute amount of samples is immensely important for understanding the structural determinants of protein function in health and disease and to develop assays and dia

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::252dd61480b71dc9406455c64af23958

Expressed Protein Ligation at Methionine: N-Terminal Attachment of Homocysteine, Ligation, and Masking

Autor: Yanxin J. Wang, Tomohiro Tanaka, John B. Warner, E. James Petersson, Anne Wagner

Publikováno v: Angewandte Chemie. 125:6330-6333

The synthesis of chemically-modified proteins is a valuable tool for the study of their function.[1] One of the most enabling protein synthesis technologies is native chemical ligation (NCL), in which a peptide thioester is reacted with a peptide bea

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5eae16b52f6f23c93096b0c88bcf9f04
https://doi.org/10.1002/ange.201302065

Comparison of strategies for non-perturbing labeling of α-synuclein to study amyloidogenesis

Autor: Conor M. Haney, Virginia M.-Y. Lee, John J. Ferrie, Rebecca F. Wissner, Dustin J. Covell, John B. Warner, Richard J. Karpowicz, Yanxin J. Wang, E. James Petersson

Publikováno v: Organicbiomolecular chemistry. 14(5)

Characterization of the amyloidogenic Parkinson's disease protein α-synuclein (αS) has proven difficult due to its structural plasticity. Here, we present a number of complementary methods to site-specifically introduce fluorescent probes to examin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b86cb8fbd4625c60a1600adb00692b2
https://pubmed.ncbi.nlm.nih.gov/27171750