Zobrazeno 1 - 4
of 4
pro vyhledávání: '"John Antonius Verhees"'
Publikováno v:
Cytotechnology
Cytotechnology, 63(4), 371-384. Springer Netherlands
Cytotechnology, 63(4), 371-384. Springer Netherlands
The efficient establishment of high protein producing recombinant mammalian cell lines is facilitated by the use of a stringent selection system. Here, we describe two methods to create a stringent selection system based on the Zeocin resistance mark
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b114157b3f8652f4d406d9743b36998
https://dare.uva.nl/personal/pure/en/publications/methods-to-create-a-stringent-selection-system-for-mammalian-cell-lines(7bd56bbd-a72c-4422-954f-92f1a2134b59).html
https://dare.uva.nl/personal/pure/en/publications/methods-to-create-a-stringent-selection-system-for-mammalian-cell-lines(7bd56bbd-a72c-4422-954f-92f1a2134b59).html
Autor:
Karien M. Hamer, Femke Hoeksema, John Antonius Verhees, Tjalling Siersma, Rik van Blokland, Michel Siep, Arie P. Otte, Jan L. den Blaauwen
Publikováno v:
Molecular Biotechnology, 48(1), 19-29. Humana Press
Molecular Biotechnology
Molecular Biotechnology
The use of high stringency selection systems often results in the induction of very few recombinant mammalian cell lines, which limits the ability to isolate a cell line with favorable characteristics. The employment of for instance STAR elements in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8ea28a9a154d12db2f14d2eea7b4620
https://hdl.handle.net/11245/1.348059
https://hdl.handle.net/11245/1.348059
Publikováno v:
Biotechnology Research International
Biotechnology Research International, 2011
Biotechnology Research International, 2011
The use of high stringency selection systems commonly results in a strongly diminished number of stably transfected mammalian cell lines. Here we placed twelve different promoters upstream of an adjacent primary promoter and tested whether this might
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7015fd2a1c550097edf25ea00ab969a5
https://pubmed.ncbi.nlm.nih.gov/21350661
https://pubmed.ncbi.nlm.nih.gov/21350661
A novel, high stringency selection system allows screening of few clones for high protein expression
Autor:
John Antonius Verhees, Tjalling Siersma, S.Y. Man, H. J. M. Van Blokland, Ted H.J. Kwaks, C. van Mer, S. Botschuijver, Richard George Antonius Bernardus Sewalt, Vincent Klaren, N.C. Teunissen, Arie P. Otte, J.W.M. Korse
Publikováno v:
Journal of Biotechnology, 128(2), 237-245. Elsevier
To obtain highly productive mammalian cell lines, often large numbers of clones need to be screened. This is largely due to low selection stringencies, creating many, but low protein producing clones. To remedy this problem, a novel, very stringent s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19dde5c2efb35fca5081e6acc1c020c5
https://dare.uva.nl/personal/pure/en/publications/a-novel-high-stringency-selection-system-allows-screening-of-few-clones-for-high-protein-expression(71a96205-6661-40ad-a972-8bbbcfdb6aa9).html
https://dare.uva.nl/personal/pure/en/publications/a-novel-high-stringency-selection-system-allows-screening-of-few-clones-for-high-protein-expression(71a96205-6661-40ad-a972-8bbbcfdb6aa9).html
