Zobrazeno 1 - 10
of 60
pro vyhledávání: '"John Aach"'
Autor:
Gleb Kuznetsov, Daniel B. Goodman, Gabriel T. Filsinger, Matthieu Landon, Nadin Rohland, John Aach, Marc J. Lajoie, George M. Church
Publikováno v:
Genome Biology, Vol 18, Iss 1, Pp 1-12 (2017)
Abstract We present a method for identifying genomic modifications that optimize a complex phenotype through multiplex genome engineering and predictive modeling. We apply our method to identify six single nucleotide mutations that recover 59% of the
Externí odkaz:
https://doaj.org/article/6d97b73a33114393895f5b605eecfd99
Autor:
Luhan Yang, Adrian W. Briggs, Wei Leong Chew, Prashant Mali, Marc Guell, John Aach, Daniel Bryan Goodman, David Cox, Yinan Kan, Emal Lesha, Venkataramanan Soundararajan, Feng Zhang, George Church
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-12 (2016)
Precision genome engineering using homology donors and the endogenous DNA break repair machinery and recently CRISPR-Cas9 targeted APOBECs have been demonstrated. Here the authors design zinc-finger and TALE chimeric deaminases and investigate editin
Externí odkaz:
https://doaj.org/article/46f1a4a987da4603908d6583916fecd1
Publikováno v:
eLife, Vol 6 (2017)
Externí odkaz:
https://doaj.org/article/a99f02c2648444c0808f32398cf2f94b
Publikováno v:
eLife, Vol 6 (2017)
The "14-day rule" for embryo research stipulates that experiments with intact human embryos must not allow them to develop beyond 14 days or the appearance of the primitive streak. However, recent experiments showing that suitably cultured human plur
Externí odkaz:
https://doaj.org/article/42e607dc25004e77b6d70f04ea84d936
Publikováno v:
Molecular Systems Biology, Vol 7, Iss 1, Pp 1-12 (2011)
Abstract Fermentation of plant biomass by microbes like Clostridium phytofermentans recycles carbon globally and can make biofuels from inedible feedstocks. We analyzed C. phytofermentans fermenting cellulosic substrates by integrating quantitative m
Externí odkaz:
https://doaj.org/article/70dfba1c34194a7793881ecef1ebed5a
Autor:
Luhan Yang, Adrian W. Briggs, Wei Leong Chew, Prashant Mali, Marc Guell, John Aach, Daniel Bryan Goodman, David Cox, Yinan Kan, Emal Lesha, Venkataramanan Soundararajan, Feng Zhang, George Church
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-1 (2017)
Nature Communications 7: Article number: 13330 (2016); Published 2 November 2016; Updated 9 October 2017. The original version of this Article contained an error in the email address of the corresponding author George Church. The correct email is gch
Externí odkaz:
https://doaj.org/article/5cf6e9e69a8e4840a2aa262d893d4b95
Publikováno v:
PLoS ONE, Vol 9, Iss 9, p e106232 (2014)
Cell-free RNA and protein synthesis (CFPS) is becoming increasingly used for protein production as yields increase and costs decrease. Advances in reconstituted CFPS systems such as the Protein synthesis Using Recombinant Elements (PURE) system offer
Externí odkaz:
https://doaj.org/article/33fd31997b8446fa91cca38afefbf3c6
Autor:
Tobias Moemke, Anthony D. Schmitt, Mike Chou, Heng Li, Fritz J. Sedlazeck, Andrew Carroll, Tobias Marschall, David Heller, Stephen Mac, Jared Maguire, John Aach, Emily Hatas, George M. Church, Arkarachai Fungtammasan, Jay Ghurye, Medhat Mahmoud, Justin M. Zook, Shilpa Garg, Xiang Zhou, Chen-Shan Chin, Haoyu Cheng, Paul Peluso
Publikováno v:
Nature Biotechnology
Haplotype-resolved or phased genome assembly provides a complete picture of genomes and their complex genetic variations. However, current algorithms for phased assembly either do not generate chromosome-scale phasing or require pedigree information,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fe3e543a8188cf01c48cf8b9a7de39fd
https://opus.bibliothek.uni-augsburg.de/opus4/frontdoor/index/index/docId/86843
https://opus.bibliothek.uni-augsburg.de/opus4/frontdoor/index/index/docId/86843
Autor:
Felix B. Pedersen, Stan Wang, Marc J. Lajoie, Verena Volf, Xavier Rios, Timothy M. Wannier, Anik Debnath, John Aach, Michael T. Laub, Devon A. Stork, Isaac D. Lutz, Gabriel T. Filsinger, Julie Zhang, Kevin Gozzi, Christopher J. Gregg, Seth L. Shipman, George M. Church, Helene M. Kuchwara
Publikováno v:
Filsinger, G T, Wannier, T M, Pedersen, F B, Lutz, I D, Zhang, J, Stork, D A, Debnath, A, Gozzi, K, Kuchwara, H, Volf, V, Wang, S, Rios, X, Gregg, C J, Lajoie, M J, Shipman, S L, Aach, J, Laub, M T & Church, G M 2021, ' Characterizing the portability of phage-encoded homologous recombination proteins ', Nature Chemical Biology, vol. 17, no. 4, pp. 394-402 . https://doi.org/10.1038/s41589-020-00710-5
Nature chemical biology
Nature chemical biology
Efficient genome editing methods are essential for biotechnology and fundamental research. Homologous recombination (HR) is the most versatile method of genome editing, but techniques that rely on host RecA-mediated pathways are inefficient and labor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ce71405ae8898e68bc616a0a56a0d02
https://portal.findresearcher.sdu.dk/da/publications/a434af18-9d3d-4fe9-be6d-13d7c0ffd667
https://portal.findresearcher.sdu.dk/da/publications/a434af18-9d3d-4fe9-be6d-13d7c0ffd667
Autor:
Kunal Jindal, Je-Hyuk Lee, Daniel Goodwin, Thomas C. Ferrante, Conor K. Camplisson, George M. Church, Shahar Alon, Eswar Prasad Ramachandran Iyer, Lauren Mifflin, Anne Cheng, Bruce A. Yankner, Richie E. Kohman, Edward S. Boyden, Songlei Liu, King Hwa Ling, Valerie Willocq, Sukanya Punthambaker, Katharina Meyer, John Aach, Jenny M. Tam, Asmamaw T Wassie, Fei Chen, Daniel Fürth, Anubhav Sinha, Andrew C. Pawlowski
Publikováno v:
Nucleic Acids Research
We present barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel insitu analyses (BOLORAMIS), a reverse transcription-free method for spatially-resolved, targeted, in situ RNA identification of single or multiple targets. BO