Optimizing complex phenotypes through model-guided multiplex genome engineering

Autor: Gleb Kuznetsov, Daniel B. Goodman, Gabriel T. Filsinger, Matthieu Landon, Nadin Rohland, John Aach, Marc J. Lajoie, George M. Church

Publikováno v: Genome Biology, Vol 18, Iss 1, Pp 1-12 (2017)

Abstract We present a method for identifying genomic modifications that optimize a complex phenotype through multiplex genome engineering and predictive modeling. We apply our method to identify six single nucleotide mutations that recover 59% of the

Externí odkaz: https://doaj.org/article/6d97b73a33114393895f5b605eecfd99

Correction: Addressing the ethical issues raised by synthetic human entities with embryo-like features

Autor: John Aach, Jeantine Lunshof, Eswar Iyer, George M Church

Publikováno v: eLife, Vol 6 (2017)

Externí odkaz: https://doaj.org/article/a99f02c2648444c0808f32398cf2f94b

Proteome‐wide systems analysis of a cellulosic biofuel‐producing microbe

Autor: Andrew C Tolonen, Wilhelm Haas, Amanda C Chilaka, John Aach, Steven P Gygi, George M Church

Publikováno v: Molecular Systems Biology, Vol 7, Iss 1, Pp 1-12 (2011)

Abstract Fermentation of plant biomass by microbes like Clostridium phytofermentans recycles carbon globally and can make biofuels from inedible feedstocks. We analyzed C. phytofermentans fermenting cellulosic substrates by integrating quantitative m

Externí odkaz: https://doaj.org/article/70dfba1c34194a7793881ecef1ebed5a

Improved cell-free RNA and protein synthesis system.

Autor: Jun Li, Liangcai Gu, John Aach, George M Church

Publikováno v: PLoS ONE, Vol 9, Iss 9, p e106232 (2014)

Cell-free RNA and protein synthesis (CFPS) is becoming increasingly used for protein production as yields increase and costs decrease. Advances in reconstituted CFPS systems such as the Protein synthesis Using Recombinant Elements (PURE) system offer

Externí odkaz: https://doaj.org/article/33fd31997b8446fa91cca38afefbf3c6

Characterizing the portability of phage-encoded homologous recombination proteins

Autor: Felix B. Pedersen, Stan Wang, Marc J. Lajoie, Verena Volf, Xavier Rios, Timothy M. Wannier, Anik Debnath, John Aach, Michael T. Laub, Devon A. Stork, Isaac D. Lutz, Gabriel T. Filsinger, Julie Zhang, Kevin Gozzi, Christopher J. Gregg, Seth L. Shipman, George M. Church, Helene M. Kuchwara

Publikováno v: Filsinger, G T, Wannier, T M, Pedersen, F B, Lutz, I D, Zhang, J, Stork, D A, Debnath, A, Gozzi, K, Kuchwara, H, Volf, V, Wang, S, Rios, X, Gregg, C J, Lajoie, M J, Shipman, S L, Aach, J, Laub, M T & Church, G M 2021, ' Characterizing the portability of phage-encoded homologous recombination proteins ', Nature Chemical Biology, vol. 17, no. 4, pp. 394-402 . https://doi.org/10.1038/s41589-020-00710-5
Nature chemical biology

Efficient genome editing methods are essential for biotechnology and fundamental research. Homologous recombination (HR) is the most versatile method of genome editing, but techniques that rely on host RecA-mediated pathways are inefficient and labor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ce71405ae8898e68bc616a0a56a0d02
https://portal.findresearcher.sdu.dk/da/publications/a434af18-9d3d-4fe9-be6d-13d7c0ffd667