Mechanical Genomic Studies Reveal the Role of d-Alanine Metabolism in Pseudomonas aeruginosa Cell Stiffness

Autor: Rishi R. Trivedi, John A. Crooks, George K. Auer, Joel Pendry, Ilona P. Foik, Albert Siryaporn, Nicholas L. Abbott, Zemer Gitai, Douglas B. Weibel

Publikováno v: mBio, Vol 9, Iss 5 (2018)

ABSTRACT The stiffness of bacteria prevents cells from bursting due to the large osmotic pressure across the cell wall. Many successful antibiotic chemotherapies target elements that alter mechanical properties of bacteria, and yet a global view of t

Externí odkaz: https://doaj.org/article/b0e1f27012c8493db4b737bc9e32c109

Rapid identification of ESKAPE bacterial strains using an autonomous microfluidic device.

Autor: Jack Y Ho, Nate J Cira, John A Crooks, Josue Baeza, Douglas B Weibel

Publikováno v: PLoS ONE, Vol 7, Iss 7, p e41245 (2012)

This article describes Bacteria ID Chips ('BacChips'): an inexpensive, portable, and autonomous microfluidic platform for identifying pathogenic strains of bacteria. BacChips consist of a set of microchambers and channels molded in the elastomeric po

Externí odkaz: https://doaj.org/article/565df6ac6fd94efa965987c428c389e1

Mechanical Genomic Studies Reveal the Role of d -Alanine Metabolism in Pseudomonas aeruginosa Cell Stiffness

Autor: Ilona P. Foik, George K. Auer, Rishi R. Trivedi, Zemer Gitai, Douglas B. Weibel, Joel Pendry, John A. Crooks, Nicholas L. Abbott, Albert Siryaporn

Publikováno v: mBio, Vol 9, Iss 5 (2018)
mBio, Vol 9, Iss 5, p e01340-18 (2018)
mBio, vol 9, iss 5
Trivedi, RR; Crooks, JA; Auer, GK; Pendry, J; Foik, IP; Siryaporn, A; et al.(2018). Mechanical Genomic Studies Reveal the Role of d-Alanine Metabolism in Pseudomonas aeruginosa Cell Stiffness.. mBio, 9(5). doi: 10.1128/mBio.01340-18. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/8tf287v6
mBio

A central question in mechanobiology is how cellular-scale structures are established and regulated. In bacteria, the cell envelope is essential for mechanical integrity, protecting against environmental stresses and bearing the load from high turgor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e8fab36f5b43f332c0e460eb431a13c
https://journals.asm.org/doi/10.1128/mBio.01340-18

Microfluidics for High School Chemistry Students

Autor: Jennifer Gilbertson, Katie Brenner, Piercen M. Oliver, Melissa Hemling, John A. Crooks, George C. Lisensky, Douglas B. Weibel

Publikováno v: Journal of Chemical Education. 91:112-115

We present a laboratory experiment that introduces high school chemistry students to microfluidics while teaching fundamental properties of acid–base chemistry. The procedure enables students to create microfluidic systems using nonspecialized equi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fd738da290b55cf79ab21a95c5c69d37
https://doi.org/10.1021/ed4003018

Snapshot Voltammetry Using a Triangular Bipolar Microelectrode

Autor: John A. Crooks, François Mavré, Richard M. Crooks, Kwok-Fan Chow, Byoung-Yong Chang

Publikováno v: Analytical Chemistry. 82:5317-5322

In this paper, we report a new electroanalytical technique we call snapshot voltammetry. This method combines the properties of bipolar electrodes with electrogenerated chemiluminescence (ECL) to provide a means for recording optical voltammograms in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1603f287715e71b6bad1c3497fd065dc
https://doi.org/10.1021/ac100846v