Robotic microinjection enables large-scale transgenic studies of Caenorhabditis elegans

Autor: Peng Pan, Michael Zoberman, Pengsong Zhang, Sharanja Premachandran, Sanjana Bhatnagar, Pallavi P. Pilaka-Akella, William Sun, Chengyin Li, Charlotte Martin, Pengfei Xu, Zefang Zhang, Ryan Li, Wesley Hung, Hua Tang, Kailynn MacGillivray, Bin Yu, Runze Zuo, Karinna Pe, Zhen Qin, Shaojia Wang, Ang Li, W. Brent Derry, Mei Zhen, Arneet L. Saltzman, John A. Calarco, Xinyu Liu

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-15 (2024)

Abstract The nematode Caenorhabditis elegans is widely employed as a model organism to study basic biological mechanisms. However, transgenic C. elegans are generated by manual injection, which remains low-throughput and labor-intensive, limiting the

Externí odkaz: https://doaj.org/article/b503551c7f214a86a2d6655e303a67ea

The transcriptional landscape of Shh medulloblastoma

Autor: Patryk Skowron, Hamza Farooq, Florence M. G. Cavalli, A. Sorana Morrissy, Michelle Ly, Liam D. Hendrikse, Evan Y. Wang, Haig Djambazian, Helen Zhu, Karen L. Mungall, Quang M. Trinh, Tina Zheng, Shizhong Dai, Ana S. Guerreiro Stucklin, Maria C. Vladoiu, Vernon Fong, Borja L. Holgado, Carolina Nor, Xiaochong Wu, Diala Abd-Rabbo, Pierre Bérubé, Yu Chang Wang, Betty Luu, Raul A. Suarez, Avesta Rastan, Aaron H. Gillmor, John J. Y. Lee, Xiao Yun Zhang, Craig Daniels, Peter Dirks, David Malkin, Eric Bouffet, Uri Tabori, James Loukides, François P. Doz, Franck Bourdeaut, Olivier O. Delattre, Julien Masliah-Planchon, Olivier Ayrault, Seung-Ki Kim, David Meyronet, Wieslawa A. Grajkowska, Carlos G. Carlotti, Carmen de Torres, Jaume Mora, Charles G. Eberhart, Erwin G. Van Meir, Toshihiro Kumabe, Pim J. French, Johan M. Kros, Nada Jabado, Boleslaw Lach, Ian F. Pollack, Ronald L. Hamilton, Amulya A. Nageswara Rao, Caterina Giannini, James M. Olson, László Bognár, Almos Klekner, Karel Zitterbart, Joanna J. Phillips, Reid C. Thompson, Michael K. Cooper, Joshua B. Rubin, Linda M. Liau, Miklós Garami, Peter Hauser, Kay Ka Wai Li, Ho-Keung Ng, Wai Sang Poon, G. Yancey Gillespie, Jennifer A. Chan, Shin Jung, Roger E. McLendon, Eric M. Thompson, David Zagzag, Rajeev Vibhakar, Young Shin Ra, Maria Luisa Garre, Ulrich Schüller, Tomoko Shofuda, Claudia C. Faria, Enrique López-Aguilar, Gelareh Zadeh, Chi-Chung Hui, Vijay Ramaswamy, Swneke D. Bailey, Steven J. Jones, Andrew J. Mungall, Richard A. Moore, John A. Calarco, Lincoln D. Stein, Gary D. Bader, Jüri Reimand, Jiannis Ragoussis, William A. Weiss, Marco A. Marra, Hiromichi Suzuki, Michael D. Taylor

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)

Sonic Hedgehog medulloblastoma (Shh-MB) comprises four subtypes each with distinct clinical traits. Here the authors characterize the genome, transcriptome, and methylome of Shh-MB subtypes, revealing a complex fusion landscape and the molecular conv

Externí odkaz: https://doaj.org/article/091429a4162845e7a18371f487596c6f

Serotonin-dependent kinetics of feeding bursts underlie a graded response to food availability in C. elegans

Autor: Kyung Suk Lee, Shachar Iwanir, Ronen B. Kopito, Monika Scholz, John A. Calarco, David Biron, Erel Levine

Publikováno v: Nature Communications, Vol 8, Iss 1, Pp 1-11 (2017)

Regulating food intake is an important physiological mechanism. Here, the authors use a custom microfluidic device to investigate feeding dynamics inC. elegans, and identify roles of serotonergic neurons in regulating bursts of feeding in response to

Externí odkaz: https://doaj.org/article/eac0a957a6d24619b1ca95b99c87377c

Two-Color Fluorescent Reporters for Analysis of Alternative Splicing

Autor: John A, Calarco, Pallavi P, Pilaka-Akella

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2537

Alternative splicing is a key layer of gene regulation that is frequently modulated in a spatiotemporal manner. As such, it is a major goal to understand the mechanisms controlling alternative splicing in specific cellular contexts. Reporters that re

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::80d80a1ebb1ee354b5b3c31d8a7314de
https://pubmed.ncbi.nlm.nih.gov/35895267

Approaches for CRISPR/Cas9 Genome Editing in C. elegans

Autor: Charlotte J, Martin, John A, Calarco

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2468

The clustered, regularly interspaced, short, palindromic repeat (CRISPR)-associated (CAS) nuclease Cas9 has been used in many organisms to generate specific mutations and transgene insertions. Here we describe our most up-to-date protocols using the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ba22ed5a6167167964f4dc23b01030fb
https://pubmed.ncbi.nlm.nih.gov/35320567