Zobrazeno 1 - 10
of 254
pro vyhledávání: '"John A Gerlt"'
Autor:
Sara Calhoun, Magdalena Korczynska, Daniel J Wichelecki, Brian San Francisco, Suwen Zhao, Dmitry A Rodionov, Matthew W Vetting, Nawar F Al-Obaidi, Henry Lin, Matthew J O'Meara, David A Scott, John H Morris, Daniel Russel, Steven C Almo, Andrei L Osterman, John A Gerlt, Matthew P Jacobson, Brian K Shoichet, Andrej Sali
Publikováno v:
eLife, Vol 7 (2018)
The functions of most proteins are yet to be determined. The function of an enzyme is often defined by its interacting partners, including its substrate and product, and its role in larger metabolic networks. Here, we describe a computational method
Externí odkaz:
https://doaj.org/article/fd3b096083a24d889b6a4ac902e9dcb6
Autor:
Suwen Zhao, Ayano Sakai, Xinshuai Zhang, Matthew W Vetting, Ritesh Kumar, Brandan Hillerich, Brian San Francisco, Jose Solbiati, Adam Steves, Shoshana Brown, Eyal Akiva, Alan Barber, Ronald D Seidel, Patricia C Babbitt, Steven C Almo, John A Gerlt, Matthew P Jacobson
Publikováno v:
eLife, Vol 3 (2014)
Metabolic pathways in eubacteria and archaea often are encoded by operons and/or gene clusters (genome neighborhoods) that provide important clues for assignment of both enzyme functions and metabolic pathways. We describe a bioinformatic approach (g
Externí odkaz:
https://doaj.org/article/987f7767be6744a18463d2a7fd3a79e8
Publikováno v:
ACS Bio & Med Chem Au, Vol 2, Iss 1, Pp 22-35 (2021)
Externí odkaz:
https://doaj.org/article/50d366fb081449e5873827e8b91f460a
Publikováno v:
ACS Bio Med Chem Au
ACS Bio & Med Chem Au, Vol 2, Iss 1, Pp 22-35 (2021)
ACS Bio & Med Chem Au, Vol 2, Iss 1, Pp 22-35 (2021)
The radical SAM superfamily (RSS), arguably the most functionally diverse enzyme superfamily, is also one of the largest with ~700K members currently in the UniProt database. The vast majority of the members have uncharacterized enzymatic activities
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3bb47ceb787926e1ed496babb5e5fe3
https://pubmed.ncbi.nlm.nih.gov/36119373
https://pubmed.ncbi.nlm.nih.gov/36119373
Publikováno v:
Microbial Genomics. 9
Pyridoxal 5’-phosphate or PLP is a cofactor derived from B6 vitamers and essential for growth in all known organisms. PLP synthesis and salvage pathways are well characterized in a few model species even though key components, such as the vitamin B
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::043f3f7a1d80f80b29e0d33f04357a26
https://doi.org/10.1099/mgen.0.000926
https://doi.org/10.1099/mgen.0.000926
Autor:
Brian S. MacTavish, Rémi Zallot, You Hu, Valérie de Crécy-Lagard, Daniel J. Payan, Steven D. Bruner, Alexander Angerhofer, Alvaro Montoya, John A. Gerlt, Qiang Li
Publikováno v:
Biochemistry
Queuosine is a structurally unique and functionally important tRNA modification, widely distributed in eukaryotes and bacteria. The final step of queuosine biosynthesis is the reduction/deoxygenation of epoxyqueuosine to form the cyclopentene motif o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::713b40f68014aab886c60bc2fced3649
https://doi.org/10.1021/acs.biochem.1c00164
https://doi.org/10.1021/acs.biochem.1c00164
Autor:
John A. Gerlt
Publikováno v:
Biochemistry. 60:3529-3538
Every reader knows that an enzyme accelerates a reaction by reducing the activation-energy barrier. However, understanding how this is achieved by the structure of the enzyme and its interactions with stable complexes and transition states and, then,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e23d75004a0b30e9868fc5e11414b581
https://doi.org/10.1021/acs.biochem.1c00211
https://doi.org/10.1021/acs.biochem.1c00211
Autor:
Justin A. North, John A. Wildenthal, Tobias J. Erb, Fred Robert Tabita, Bradley S. Evans, John A. Gerlt, Kathryn M. Byerly
Publikováno v:
Mol Microbiol
S-adenosyl-l-methionine (SAM) is a necessary cosubstrate for numerous essential enzymatic reactions including protein and nucleotide methylations, secondary metabolite synthesis and radical-mediated processes. Radical SAM enzymes produce 5'-deoxyaden
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd59254aa9c66fedf30c7179e0b6a316
https://doi.org/10.1111/mmi.14459
https://doi.org/10.1111/mmi.14459
Publikováno v:
Journal of Molecular Biology. :168018
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4ef00306acf92e783d4b501f040c0fff
https://doi.org/10.1016/j.jmb.2023.168018
https://doi.org/10.1016/j.jmb.2023.168018
Publikováno v:
Biochemistry
The assignment of functions to uncharacterized proteins discovered in genome projects requires easily accessible tools and computational resources for large-scale, user-friendly leveraging of the protein, genome, and metagenome databases by experimen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b84f80bc36e074514dfc5296798ce5c
https://doi.org/10.1021/acs.biochem.9b00735
https://doi.org/10.1021/acs.biochem.9b00735