Molecular design of controllable recombinant adeno-associated virus (AAV) expression systems for enhanced vector production.

Autor: Johari YB; Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, UK., Pohle TH; Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, UK.; Syngensys Ltd., Sheffield, UK., Whitehead J; Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, UK., Scarrott JM; Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, UK., Liu P; Cell Line Development, REGENXBIO Inc., Rockville, Maryland, USA., Mayer A; Cell Line Development, REGENXBIO Inc., Rockville, Maryland, USA., James DC; Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, UK.; Syngensys Ltd., Sheffield, UK.

Publikováno v: Biotechnology journal [Biotechnol J] 2024 Jun; Vol. 19 (6), pp. e2300685.

Engineering of the CMV promoter for controlled expression of recombinant genes in HEK293 cells.

Autor: Johari YB; Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, UK., Scarrott JM; Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, UK., Pohle TH; Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, UK., Liu P; Cell Line Development, REGENXBIO Inc., Rockville, Maryland, USA., Mayer A; Cell Line Development, REGENXBIO Inc., Rockville, Maryland, USA., Brown AJ; Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, UK.; Syngensys Ltd., Sheffield, UK., James DC; Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, UK.; Syngensys Ltd., Sheffield, UK.

Publikováno v: Biotechnology journal [Biotechnol J] 2022 Aug; Vol. 17 (8), pp. e2200062. Date of Electronic Publication: 2022 May 06.

Production of trimeric SARS-CoV-2 spike protein by CHO cells for serological COVID-19 testing.

Autor: Johari YB; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Jaffé SRP; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Scarrott JM; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Johnson AO; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Mozzanino T; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Pohle TH; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Maisuria S; Department of Immunology, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, UK., Bhayat-Cammack A; Department of Immunology, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, UK., Lambiase G; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Brown AJ; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Tee KL; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Jackson PJ; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Wong TS; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Dickman MJ; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Sargur RB; Department of Immunology, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, UK., James DC; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK.

Publikováno v: Biotechnology and bioengineering [Biotechnol Bioeng] 2021 Feb; Vol. 118 (2), pp. 1013-1021. Date of Electronic Publication: 2020 Nov 11.

Promoter engineering enables precise metabolic regulation towards efficient β-elemene production in Ogataea polymorpha.

Autor: Min Ye^1,2, Jiaoqi Gao^1,3,4, Jingjing Li^1,4, Wei Yu^1,4, Fan Bai^1,4, Zhou, Yongjin J.^1,3,4 zhouyongjin@dicp.ac.cn

Publikováno v: Synthetic & Systems Biotechnology. Jun2024, Vol. 9 Issue 2, p234-241. 8p.

Metabolic phenotyping of CHO cells varying in cellular biomass accumulation and maintenance during fed-batch culture.

Autor: Fernandez-Martell A; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Johari YB; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., James DC; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK.

Publikováno v: Biotechnology and bioengineering [Biotechnol Bioeng] 2018 Mar; Vol. 115 (3), pp. 645-660. Date of Electronic Publication: 2017 Nov 28.