Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Johannes van der Winden"'
Publikováno v:
Plant Physiology. 139:1586-1596
Sixteen distinct sites distributed on all five Arabidopsis (Arabidopsis thaliana) chromosomes have been tagged using different fluorescent proteins and one of two different bacterial operator-repressor systems: (1) a yellow fluorescent protein-Tet re
Publikováno v:
Plant Molecular Biology Reporter. 21:9-19
The bacterial tetracycline operator/repressor (tetO/TetR) system and enhanced yellow fluorescent protein (EYFP) have been adapted for use inArabidopsis to visualize tagged T-DNAs in interphase nuclei of living cells. The 2-component system was assemb
Autor:
M. Florian Mette, Werner Aufsatz, Marjori Matzke, Antonius J. M. Matzke, Johannes van der Winden
Publikováno v:
Proceedings of the National Academy of Sciences. 99:16499-16506
In plants, double-stranded RNA that is processed to short RNAs ≈21–24 nt in length can trigger two types of epigenetic gene silencing. Posttranscriptional gene silencing, which is related to RNA interference in animals and quelling in fungi, invo
Publikováno v:
Plant Physiology. 130:6-9
Mining the Arabidopsis genome for transposable elements (TEs) by DNA sequence similarity searches and analysis protocols is revealing previously unidentified families of TEs and providing insights into TE structure, mobility, distribution, and divers
Publikováno v:
Plant Methods, Vol 6, Iss 1, p 2 (2010)
Plant Methods
Plant Methods
Background Interphase chromosome organization and dynamics can be studied in living cells using fluorescent tagging techniques that exploit bacterial operator/repressor systems and auto-fluorescent proteins. A nuclear-localized Repressor Protein-Fluo
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 463
Fluorescence tagging of genomic sites through the use of bacterial operator/repressor systems combined with fluorescent proteins permits high-resolution analysis of interphase chromosomes in living cells. This technique has been used to study interph
Autor:
Tatsuo Kanno, Antonius J. M. Matzke, Ulf Naumann, Marjori Matzke, Etienne Bucher, Johannes van der Winden, Lucia Daxinger
Publikováno v:
The EMBO journal. 28(1)
We used a transgene system to study spreading of RNA-directed DNA methylation (RdDM) during transcriptional gene silencing in Arabidopsis thaliana. Forward and reverse genetics approaches using this system delineated a stepwise pathway for the biogen
Autor:
Marjori Matzke, Antonius J. M. Matzke, Johannes van der Winden, Bruno Huettel, Lucia Daxinger, Etienne Bucher, Tatsuo Kanno
Publikováno v:
Biochimica et biophysica acta. 1769(5-6)
RNA-directed DNA methylation, which is one of several RNAi-mediated pathways in the nucleus, has been highly elaborated in the plant kingdom. RNA-directed DNA methylation requires for the most part conventional DNA methyltransferases, histone modifyi
Autor:
Marjori Matzke, Werner Aufsatz, Stephen E. Schauer, István Papp, Johannes van der Winden, M. Florian Mette, Antonius J. M. Matzke, Animesh Ray, Lucia Daxinger
Publikováno v:
Plant physiology. 132(3)
The Arabidopsis genome encodes four Dicer-like (DCL) proteins, two of which contain putative nuclear localization signals. This suggests one or more nuclear pathways for processing double-stranded (ds) RNA in plants. To study the subcellular location
Autor:
Christian, Kunz, Jarunya, Narangajavana, Johannes, Jakowitsch, Young-Doo, Park, T René, Delon, Ales, Kovarik, Blazena, Koukalová, Johannes, van der Winden, Eduardo, Moscone, Werner, Aufsatz, M Florian, Mette, Marjori, Matzke, Antonius J M, Matzke
Publikováno v:
Plant molecular biology. 52(1)
To test the influence of a Nicotiana tomentosiformis repetitive sequence (R8.3) on transgene expression in N. sylvestris and in N. sylvestris-N. tomentosiformis hybrids, the R8.3 sequence was placed upstream of a nopaline synthase promoter (NOSpro)-N