Zobrazeno 1 - 10
of 24
pro vyhledávání: '"Johannes Oehlke"'
Autor:
Eberhard Krause, Giovanna Valenti, Enno Klussmann, Johannes Oehlke, Maria Svelto, Walter Rosenthal, Grazia Tamma
Publikováno v:
Journal of Cell Science. 134
Publikováno v:
Current Drug Delivery. 8:526-533
The ability of peptide nucleic acids (PNA) to enter and to cross filter-grown MDCK, HEK and CHO cells was studied by means of a protocol based on capillary electrophoresis combined with laser-induced fluorescence detection. The used approach avoided
Autor:
Burkhard Wiesner, Angelika Ehrlich, Stephan Pritz, Eberhard Krause, Michael Beyermann, Johannes Oehlke
Publikováno v:
Nucleic Acid Therapeutics. 21:285-291
Evidence is presented that components of fetal calf serum (FCS) can significantly enhance the splicing correction activity of peptide nucleic acids (PNA) in HeLa pLuc 705 cells. The effect proved more pronounced for PNAs bearing fluorescence tags and
Publikováno v:
Journal of Peptide Science. 16:71-80
A 12-mer peptide nucleic acid (PNA) directed against the nociceptin/orphanin FQ receptor mRNA was disulfide bridged with various peptides without and with cell-penetrating features. The cellular uptake and the antisense activity of these conjugates w
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. 1667:222-228
The influence of the peptide-to-cell ratio and energy depletion on uptake and degradation of the cell-penetrating peptides (CPPs) MAP (model amphipathic peptide) was investigated. The intracellular concentration of the CPPs, MAP and penetratin was mo
Autor:
Hartmut Berger, Gerd Wallukat, Yvonne Wolf, Johannes Oehlke, Burkhard Wiesner, Michael Bienert, Angelika Ehrlich
Publikováno v:
European Journal of Biochemistry. 271:3043-3049
In order to evaluate the ability of the cell-penetrating α-helical amphipathic model peptide KLALKLALKALKAALKLA-NH2 (MAP) to deliver peptide nucleic acids (PNAs) into mammalian cells, MAP was covalently linked to the 12-mer PNA 5′-GGAGCAGGAAAG-3
Autor:
Burkhard Wiesner, Michael Bienert, E. Klauschenz, P. Birth, Michael Beyermann, A. Oksche, Johannes Oehlke
Publikováno v:
European Journal of Biochemistry. 269:4025-4032
The uptake by mammalian cells of phosphorothioate oligonucleotides was compared with that of their respective complexes or conjugates with cationic, cell-penetrating model peptides of varying helix-forming propensity and amphipathicity. An HPLC-based
Publikováno v:
Letters in Peptide Science. 8:29-34
Extracellular administration of a membranepermeable model peptide containing the tripeptide sequence,SLV, at the C-terminus to human endothelial and kidney cellsresulted in an induction of caspase-8 (FLICE), the apicalenzyme of the apoptosis cascade.
Publikováno v:
Letters in Peptide Science. 8:29-34
Publikováno v:
European Journal of Biochemistry. 267:6043-6050
The cellular uptake of a peptide set derived from membrane-permeable α-helical amphipathic peptides by stepwise alterations of structure forming propensity and charge was studied by confocal laser scanning microscopy (CLSM) combined with HPLC. For C