Translation of non-standard codon nucleotides reveals minimal requirements for codon-anticodon interactions

Autor: Thomas Philipp Hoernes, Klaus Faserl, Michael Andreas Juen, Johannes Kremser, Catherina Gasser, Elisabeth Fuchs, Xinying Shi, Aaron Siewert, Herbert Lindner, Christoph Kreutz, Ronald Micura, Simpson Joseph, Claudia Höbartner, Eric Westhof, Alexander Hüttenhofer, Matthias David Erlacher

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-12 (2018)

The recognition of the mRNA codon by the tRNA anticodon is crucial for protein synthesis. Here the authors introduce non-standard nucleotides in bacterial and eukaryotic mRNA to reveal the minimal hydrogen bond requirement of codon-anticodon interact

Externí odkaz: https://doaj.org/article/ffffbc1b3a5340bdb59935247e94c0d4

Bridging the Antimicrobial Activity of Two Lactoferricin Derivatives in E. coli and Lipid-Only Membranes

Autor: Karl Lohner, Georg Pabst, Enrico F. Semeraro, Johannes Kremser, Johannes Mandl, Lisa Marx, Nermina Malanovic, Moritz P. K. Frewein

Publikováno v: Frontiers in Medical Technology

We coupled the antimicrobial activity of two well-studied lactoferricin derivatives, LF11-215 and LF11-324, in Escherichia coli and different lipid-only mimics of its cytoplasmic membrane using a common thermodynamic framework for peptide partitionin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22520c2ebc3780417a82e398c9d99da7
http://europepmc.org/articles/PMC8757871

Translation of non-standard codon nucleotides reveals minimal requirements for codon-anticodon interactions

Autor: Ronald Micura, Aaron Siewert, Eric Westhof, Christoph Kreutz, Johannes Kremser, Catherina Gasser, Thomas Philipp Hoernes, Elisabeth Fuchs, Alexander Hüttenhofer, Michael Andreas Juen, Herbert Lindner, Klaus Faserl, Xinying Shi, Claudia Höbartner, Matthias D. Erlacher, Simpson Joseph

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
Nature Communications
Nature Communications, Nature Publishing Group, 2018, 9 (1), ⟨10.1038/s41467-018-07321-8⟩

The precise interplay between the mRNA codon and the tRNA anticodon is crucial for ensuring efficient and accurate translation by the ribosome. The insertion of RNA nucleobase derivatives in the mRNA allowed us to modulate the stability of the codon-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ff84538131762a5015fd2942f271a57
http://link.springer.com/article/10.1038/s41467-018-07321-8

NMR resonance assignments for the SAM/SAH-binding riboswitch RNA bound to S-adenosylhomocysteine

Autor: Heiko Keller, Jens Wöhnert, Michael Andreas Juen, Christoph Kreutz, Elke Duchardt-Ferner, Elisabeth Strebitzer, Johannes Kremser, Jan Philip Wurm, A. Katharina Weickhmann

Publikováno v: Biomolecular NMR Assignments. 12:329-334

Riboswitches are structured RNA elements in the 5'-untranslated regions of bacterial mRNAs that are able to control the transcription or translation of these mRNAs in response to the specific binding of small molecules such as certain metabolites. Ri

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf72bcdc135664bdeee7767e5519eff5
https://doi.org/10.1007/s12104-018-9834-3

Chemical synthesis and NMR spectroscopy of long stable isotope labelled RNA

Autor: Heidelinde Glasner, Felix Nußbaumer, Christoph Kreutz, Michael Andreas Juen, Elisabeth Strebitzer, Kathrin Breuker, Johannes Kremser, Raphael Plangger

Publikováno v: Chemical Communications. 53:12938-12941

We showcase the high potential of the 2′-cyanoethoxymethyl (CEM) methodology to synthesize RNAs with naturally occurring modified residues carrying stable isotope (SI) labels for NMR spectroscopic applications. The method was applied to synthesize

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd7632d615bd497d8024314a8c34b2f9
https://doi.org/10.1039/c7cc06747j