Zobrazeno 1 - 10 of 29 pro vyhledávání: '"Johannes Gierlich"'
1
Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry
Autor: Thomas Carell, Johannes Gierlich, Glenn A. Burley, Philipp M. E. Gramlich, Katrin Gutsmiedl, Alexandra Schmidt
Publikováno v: Chemistry - A European Journal. 13:9486-9494
We report the combination of "click chemistry" with PCR by using alkyne-modified triphosphates for efficient and homogeneous labeling of DNA. A series of modified PCR products of different lengths (300, 900, and 2000 base pairs) were prepared by usin
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4da58359c586c9024937fa100fb5c833
https://doi.org/10.1002/chem.200700502
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2
Pronounced Effect of DNA Hybridization on Click Reaction Efficiency
Autor: Johannes Gierlich, Glenn A. Burley, Christian T. Wirges, Thomas Carell, Katrin Gutsmiedl, Philipp M. E. Gramlich
Publikováno v: QSAR & Combinatorial Science. 26:1159-1164
We report about the efficiency of the click reaction directly on PCR products containing alkyne-modified uridine nucleosides. Galactose azide was clicked onto single-stranded and double-stranded 300mer DNA in order to investigate the efficiency of su
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4fb47dfa619cc9d4fc1822f68eaa9754
https://doi.org/10.1002/qsar.200740069
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9
Synthesis of Modified DNA by PCR with Alkyne-Bearing Purines Followed by a Click Reaction
Autor: Thomas Carell, Christian T. Wirges, Johannes Gierlich, Philipp M. E. Gramlich
Publikováno v: Organic Letters. 10:249-251
Alkyne-bearing deazapurine triphosphates were prepared and successfully incorporated into DNA using the polymerase chain reaction (PCR). The obtained alkyne-labeled DNA was successfully used in a click reaction with galactose azide.
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14111102c6a44b734bc914f5b04cd204
https://doi.org/10.1021/ol7026015
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10
Cyclobutane Pyrimidine Dimers as UV-Induced DNA Lesions
Autor: Marcus G. Friedel, Thomas Carell, Johannes Gierlich
Publikováno v: Patai's Chemistry of Functional Groups
1 Introduction 2 Synthesis of Uracil and Thymine CPDS as the Basis for the Development of Model Compounds 3 CPD Cleavage Investigations 4 Investigations with Covalently Linked Model Compounds 5 Synthesis of Uracil and Thymine CPD Phosphoramidite Buil
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::07993e3214fd68cf5f404d2d2e6206c6
https://doi.org/10.1002/9780470682531.pat0336
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