Zobrazeno 1 - 10
of 32
pro vyhledávání: '"Johannes Andries Roubos"'
Publikováno v:
Ciurkot, K W, Gorochowski, T E, Roubos, J A & Verwaal, R 2021, ' Efficient multiplexed gene regulation in Saccharomyces cerevisiae using dCas12a ', Nucleic Acids Research, vol. 49, no. 13, gkab529, pp. 7775-7790 . https://doi.org/10.1093/nar/gkab529
Nucleic Acids Research
Nucleic Acids Research
CRISPR Cas12a is an RNA-programmable endonuclease particularly suitable for gene regulation. This is due to its preference for T-rich PAMs that allows it to more easily target AT-rich promoter sequences, and built-in RNase activity which can process
Autor:
Bojana Stevović, Roman Jerala, Helena Gradišar, Johannes Andries Roubos, Tina Fink, Mojca Benčina, Rok Gaber, René Verwaal
Publikováno v:
AMB Express
AMB Express, Vol 10, Iss 1, Pp 1-10 (2020)
AMB Express, Vol 10, Iss 1, Pp 1-10 (2020)
The clustering of biosynthetic enzymes is used in nature to channel reaction products and increase the yield of compounds produced by multiple reaction steps. The coupling of multiple enzymes has been shown to increase the biosynthetic product yield.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af79270b2c7de199f2a096926971e363
https://doi.org/10.21203/rs.3.rs-21832/v2
https://doi.org/10.21203/rs.3.rs-21832/v2
Publikováno v:
Yeast. 35:201-211
Cpf1 represents a novel single RNA-guided CRISPR/Cas endonuclease system suitable for genome editing with distinct features compared with Cas9. We demonstrate the functionality of three Cpf1 orthologues - Acidaminococcus spp. BV3L6 (AsCpf1), Lachnosp
Autor:
John van der Oost, Johannes Andries Roubos, René Verwaal, Belén Adiego-Pérez, Pascale Daran-Lapujade, Paola Randazzo, Jean-Marc Daran
Publikováno v:
FEMS Microbiology Letters 366 (2019) 8
FEMS Microbiology Letters
FEMS Microbiology Letters, 366(8)
FEMS Microbiology Letters
FEMS Microbiology Letters, 366(8)
Microbial production of chemical compounds often requires highly engineered microbial cell factories. During the last years, CRISPR-Cas nucleases have been repurposed as powerful tools for genome editing. Here, we briefly review the most frequently u
Autor:
Irem Avcilar-Kucukgoze, Thomas E. Gorochowski, Roel A. L. Bovenberg, Zoya Ignatova, Johannes Andries Roubos
Publikováno v:
Gorochowski, T E, Avcilar-Kucukgoze, I, Bovenberg, R A L, Roubos, J A & Ignatova, Z 2016, ' A Minimal Model of Ribosome Allocation Dynamics Captures Trade-offs in Expression between Endogenous and Synthetic Genes ', ACS Synthetic Biology, vol. 5, no. 7, pp. 710-720 . https://doi.org/10.1021/acssynbio.6b00040
ACS Synthetic Biology, 5(7):6b00040, 710-720. AMER CHEMICAL SOC
ACS Synthetic Biology, 5(7):6b00040, 710-720. AMER CHEMICAL SOC
Cells contain a finite set of resources that must be distributed across many processes to ensure survival. Among them, the largest proportion of cellular resources is dedicated to protein translation. Synthetic biology often exploits these resources
Autor:
D. Benjamin Gordon, Bianca Elisabeth Maria Gielesen, Zheng Zhao, Liang Wu, Eric M. Young, Johannes Andries Roubos, Christopher A. Voigt
Publikováno v:
Metabolic engineering. 48
Metabolic engineering requires multiple rounds of strain construction to evaluate alternative pathways and enzyme concentrations. Optimizing multigene pathways stepwise or by randomly selecting enzymes and expression levels is inefficient. Here, we a
Autor:
Walter Pirovano, Derek Butler, Adalberto Costessi, Johannes Andries Roubos, Ali May, Marc Kolkman, Emiel Ver Loren van Themaat, Bartholomeus van den Bogert
Publikováno v:
FEMS microbiology letters. 365(16)
Industrial biotechnology develops and applies microorganisms for the production of bioproducts and enzymes with applications ranging from food and feed ingredients and processing to bio-based chemicals, biofuels and pharmaceutical products. Next gene
Publikováno v:
Nucleic Acids Research
Wohlgemuth, S E, Gorochowski, T E & Roubos, J A 2013, ' Translational sensitivity of the Escherichia coli genome to fluctuating tRNA availability ', Nucleic Acids Research, vol. 41, no. 17, pp. 8021-33 . https://doi.org/10.1093/nar/gkt602
Wohlgemuth, S E, Gorochowski, T E & Roubos, J A 2013, ' Translational sensitivity of the Escherichia coli genome to fluctuating tRNA availability ', Nucleic Acids Research, vol. 41, no. 17, pp. 8021-33 . https://doi.org/10.1093/nar/gkt602
The synthesis of protein from messenger RNA during translation is a highly dynamic process that plays a key role in controlling the efficiency and fidelity of genome-wide protein expression. The availability of aminoacylated transfer RNA (tRNA) is a
Autor:
M. Meijer, Herman Jan Pel, Jasmyn Pangilinan, Jette Thykaer, Gerhard H. Braus, Scott E. Baker, Rob Samson, Jens Christian Frisvad, Kristian Fog Nielsen, Erika Lindquist, Susanna A. Braus-Stromeyer, Margarita Salazar, Peter J. I. van de Vondervoort, Peter J. Schaap, Michael Lynge Nielsen, Diego Martinez, Susan Lisette Meijer, Johannes Maarten Van Den Brink, Jakob Blæsbjerg Nielsen, Johannes Andries Roubos, Hein Stam, Jon K. Magnuson, Noël Nicolaas Maria Elisabeth Van Peij, Lars Kongsbak Poulsen, Adrian Tsang, Alex Atkins, Asaf Salamov, Ziyu Dai, Jane Grimwood, Harris Shapiro, Igor V. Grigoriev, Piet W.M. van Dijck, Susan Lucas, Andrea Aerts, Luis M. Corrochano, Hildegard Henna Menke, Albert J. J. van Ooyen, David E. Culley, Gerald Hofmann, Mikael Rørdam Andersen, Randy M. Berka, Jens Nielsen, Linda L. Lasure, Yigong Lou, Kaj Albermann, Christian P. Kubicek, Richard Albang
Publikováno v:
Genome Research 21 (2011) 6
idUS. Depósito de Investigación de la Universidad de Sevilla
instname
Genome Research, 21(6), 885-897. Cold Spring Harbor Laboratory Press
Genome Research, 21(6), 885-897
idUS. Depósito de Investigación de la Universidad de Sevilla
instname
Genome Research, 21(6), 885-897. Cold Spring Harbor Laboratory Press
Genome Research, 21(6), 885-897
The filamentous fungus Aspergillus niger exhibits great diversity in its phenotype. It is found globally, both as marine and terrestrial strains, produces both organic acids and hydrolytic enzymes in high amounts, and some isolates exhibit pathogenic
Autor:
Paul S. Dyer, Andrew Plumridge, Johannes Andries Roubos, J. Stark, David B. Archer, Lee Shunburne, Malcolm Stratford, Michaela Novodvorska, Petter Melin, Hein Stam, Hildegard Henna Menke
Publikováno v:
Fungal Genetics and Biology. 47:683-692
The ability to resist anti-microbial compounds is of key evolutionary benefit to microorganisms. Aspergillus niger has previously been shown to require the activity of a phenylacrylic acid decarboxylase (encoded by padA1) for the decarboxylation of t