Metabolic enzyme clustering by coiled coils improves the biosynthesis of resveratrol and mevalonate

Autor: Tina Fink, Bojana Stevović, René Verwaal, Johannes A. Roubos, Rok Gaber, Mojca Benčina, Roman Jerala, Helena Gradišar

Publikováno v: AMB Express, Vol 10, Iss 1, Pp 1-10 (2020)

Abstract The clustering of biosynthetic enzymes is used in nature to channel reaction products and increase the yield of compounds produced by multiple reaction steps. The coupling of multiple enzymes has been shown to increase the biosynthetic produ

Externí odkaz: https://doaj.org/article/eafc2fc0d72845db91f1a302bce6ded8

Efficient multiplexed gene regulation in Saccharomyces cerevisiae using dCas12a

Autor: René Verwaal, Klaudia W Ciurkot, Thomas E. Gorochowski, Johannes Andries Roubos

Publikováno v: Ciurkot, K W, Gorochowski, T E, Roubos, J A & Verwaal, R 2021, ' Efficient multiplexed gene regulation in Saccharomyces cerevisiae using dCas12a ', Nucleic Acids Research, vol. 49, no. 13, gkab529, pp. 7775-7790 . https://doi.org/10.1093/nar/gkab529
Nucleic Acids Research

CRISPR Cas12a is an RNA-programmable endonuclease particularly suitable for gene regulation. This is due to its preference for T-rich PAMs that allows it to more easily target AT-rich promoter sequences, and built-in RNase activity which can process

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c64301b120308cd6c40facea8754c32
https://doi.org/10.1093/nar/gkab529

Exploring sequence characteristics related to high-level production of secreted proteins in Aspergillus niger.

Autor: Bastiaan A van den Berg, Marcel J T Reinders, Marc Hulsman, Liang Wu, Herman J Pel, Johannes A Roubos, Dick de Ridder

Publikováno v: PLoS ONE, Vol 7, Iss 10, p e45869 (2012)

Protein sequence features are explored in relation to the production of over-expressed extracellular proteins by fungi. Knowledge on features influencing protein production and secretion could be employed to improve enzyme production levels in indust

Externí odkaz: https://doaj.org/article/7e77c22f95304804bfce54f403bcb041

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Autor: Klaudia, Ciurkot, Brenda, Vonk, Thomas E, Gorochowski, Johannes A, Roubos, René, Verwaal

Publikováno v: Journal of visualized experiments : JoVE. (147)

High efficiency, ease of use and versatility of the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system has facilitated advanced genetic modification of Saccharomyces cerevisiae, a model organism

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::cc8d9f3d57948ba6d8aba7245dc8d336
https://pubmed.ncbi.nlm.nih.gov/31205318

A Minimal Model of Ribosome Allocation Dynamics Captures Trade-offs in Expression between Endogenous and Synthetic Genes

Autor: Irem Avcilar-Kucukgoze, Thomas E. Gorochowski, Roel A. L. Bovenberg, Zoya Ignatova, Johannes Andries Roubos

Publikováno v: Gorochowski, T E, Avcilar-Kucukgoze, I, Bovenberg, R A L, Roubos, J A & Ignatova, Z 2016, ' A Minimal Model of Ribosome Allocation Dynamics Captures Trade-offs in Expression between Endogenous and Synthetic Genes ', ACS Synthetic Biology, vol. 5, no. 7, pp. 710-720 . https://doi.org/10.1021/acssynbio.6b00040
ACS Synthetic Biology, 5(7):6b00040, 710-720. AMER CHEMICAL SOC

Cells contain a finite set of resources that must be distributed across many processes to ensure survival. Among them, the largest proportion of cellular resources is dedicated to protein translation. Synthetic biology often exploits these resources

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ad0700eb4a8cdc6af45dfdc3b2dd802
https://hdl.handle.net/11370/64bfd342-7db2-4941-8440-2221d3739ba6