Hierarchical dynamics in allostery following ATP hydrolysis monitored by single molecule FRET measurements and MD simulations

Autor: Thorsten Hugel, Steffen Wolf, Gerhard Stock, Björn Hellenkamp, Johann Thurn, Benedikt Sohmen

Publikováno v: Chemical Science

We report on a study that combines advanced fluorescence methods with molecular dynamics (MD) simulations to cover timescales from nanoseconds to milliseconds for a large protein. This allows us to delineate how ATP hydrolysis in a protein causes all

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cdf03fa0fc987d463682ead6f940b06
https://doi.org/10.1039/d0sc06134d

A Novel Single Frequency, Pulsed UV Source For Airborne Direct Detection Wind Lidar

Autor: Johann Thurn, Raoul-Amadeus Lorbeer, Peter Mahnke, Matthias Damm, Oliver Kliebisch

Publikováno v: Optica Advanced Photonics Congress 2022.

We have conceptualized a compact UV source with pulse parameters optimized for airborne clear air turbulence detection. An amplified single frequency ns-Nd:YAG source is frequency-tripled to 355 nm, > 2.5W at 3 kHz pulse repetition frequency.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a61da9d634d13e510c4b57d13d150e0a
https://doi.org/10.1364/assl.2022.jm4a.10

Temperature dependence of the conversion efficiency of photochromic perylene bisimide dithienylcyclopentene triads embedded in a polymer

Autor: Jürgen Köhler, Johannes Maier, Martti Pärs, Mukundan Thelakkat, Johann Thurn, Katja Gräf

Publikováno v: Physical Chemistry Chemical Physics. 19:26065-26071

Photochromic molecules that are covalently linked to a strong fluorophore combine the requirements of external control and strong fluorescence, which will become increasingly important for super-resolution microscopy techniques based on single molecu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d0a59d2840b776c67f32ddc6396bf4c
https://doi.org/10.1039/c7cp03634e

Precision and accuracy of single-molecule FRET measurements-a multi-laboratory benchmark study

Autor: Taekjip Ha, Tim Schröder, Philip Tinnefeld, Marcia Levitus, Benjamin Schuler, Christian G. Hübner, Björn Hellenkamp, Christian A. Hanke, Markus Götz, Enrico Gratton, Johannes Hohlbein, Benjamin Ambrose, Sonja Schmid, Jens Michaelis, Anders Barth, Soheila Rezaei Adariani, Mark E. Bowen, Carel Fijen, Eleni Kallis, Edward A. Lemke, Jelle Hendrix, Victoria Birkedal, Thuy T.M. Ngo, Ralf Kühnemuth, Bettina Wünsch, Lasse L. Hildebrandt, Claus A. M. Seidel, Timothy D. Craggs, Henning Seidel, Georg Krainer, Michael Schlierf, Swati Tyagi, Hugo Sanabria, Mikayel Aznauryan, Niels Vandenberk, Giorgos Gouridis, Verena Hirschfeld, Daniel Nettels, Jae-Yeol Kim, Inna S. Yanez-Orozco, Pengyu Hao, Achillefs N. Kapanidis, Brié Levesque, Thorsten Hugel, James J. McCann, Nam Ki Lee, Oleg Opanasyuk, Andreas Hartmann, Johann Thurn, Hongtao Chen, Tobias Eilert, Lisa Streit, Don C. Lamb, Carlheinz Röcker, Ruoyi Qiu, Keith Weninger, Christian Gebhardt, Thorben Cordes, Nicole C. Robb, Nikolaus Naredi-Rainer, Andrés Manuel Vera, Boyang Hua, Olga Doroshenko

Publikováno v: Nature Methods, 15(9), 669-676. Nature Publishing Group
Nature methods
Nature Methods 15 (2018) 9
Hellenkamp, Björn; Schmid, Sonja; Doroshenko, Olga; Opanasyuk, Oleg; Kühnemuth, Ralf; Rezaei Adariani, Soheila; et al.(2018). Precision and accuracy of single-molecule FRET measurements-a multi-laboratory benchmark study.. Nature methods, 15(9), 669-676. doi: 10.1038/s41592-018-0085-0. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/82n6186z
Nature Methods
Hellenkamp, B, Schmid, S, Doroshenko, O, Opanasyuk, O, Kuehnemuth, R, Adariani, S R, Ambrose, B, Aznauryan, M, Barth, A, Birkedal, V, Bowen, M E, Chen, H, Cordes, T, Eilert, T, Fijen, C, Gebhardt, C, Goetz, M, Gouridis, G, Gratton, E, Ha, T, Hao, P, Hanke, C A, Hartmann, A, Hendrix, J, Hildebrandt, L L, Hirschfeld, V, Hohlbein, J, Hua, B, Huebner, C G, Kallis, E, Kapanidis, A N, Kim, J-Y, Krainer, G, Lamb, D C, Lee, N K, Lemke, E A, Levesque, B, Levitus, M, McCann, J J, Naredi-Rainer, N, Nettels, D, Thuy Ngo, T, Qiu, R, Robb, N C, Roecker, C, Sanabria, H, Schlierf, M, Schroeder, T, Schuler, B, Seidel, H, Streit, L, Thurn, J, Tinnefeld, P, Tyagi, S, Vandenberk, N, Vera, A M, Weninger, K R, Wunsch, B, Yanez-Orozco, I S, Michaelis, J, Seidel, C A M, Craggs, T D & Hugel, T 2018, ' Precision and accuracy of single-molecule FRET measurements-a multi-laboratory benchmark study ', Nature Methods, vol. 15, no. 9, pp. 669-676 . https://doi.org/10.1038/s41592-018-0085-0
Nature Methods, 15(9), 669-676
Nature Methods (online), 15(9)

Single-molecule Forster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However, generalized protocols and FRET standards to ensure the reproducibili

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72edbb856467f1a0f8cea053565b29e1
https://research.rug.nl/en/publications/6b30271d-1e88-40c6-a10a-544c2685cb93

Single-Molecule Fluorescence Measurements of Transient Protein Complexes Determined via Diffusion-Independent Microfluidic Mixing

Autor: Thorsten Hugel, Johann Thurn, Björn Hellenkamp

Publikováno v: Biophysical Journal. 116:163a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::46e86ddf81e4ee2405a1812c837dc0b5
https://doi.org/10.1016/j.bpj.2018.11.907