Proteases Underground: Analysis of the Maize Root Apoplast Identifies Organ Specific Papain-Like Cysteine Protease Activity

Autor: Jan Schulze Hüynck, Farnusch Kaschani, Karina van der Linde, Sebastian Ziemann, André N. Müller, Thomas Colby, Markus Kaiser, Johana C. Misas Villamil, Gunther Doehlemann

Publikováno v: Frontiers in Plant Science, Vol 10 (2019)

Plant proteases are key regulators of plant cell processes such as seed development, immune responses, senescence and programmed cell death (PCD). Apoplastic papain-like cysteine proteases (PL) are hubs in plant-microbe interactions and play an impor

Externí odkaz: https://doaj.org/article/69a68c306c0946c69b0d3676cfd1ec8b

Combination of transcriptomic, proteomic and degradomic profiling reveals common and distinct patterns of pathogen-induced cell death in maize

Autor: Sina Barghahn, Georgios Saridis, Melissa Mantz, Ute Meyer, Jaqueline C Mellüh, Johana C Misas Villamil, Pitter F Huesgen, Gunther Doehlemann

Regulated cell death (RCD) is crucial for plant development, as well as in decision-making in plant-microbe interactions. Previous studies revealed components of the molecular network controlling RCD, including different proteases. However, the ident

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4d4b8d936380312405e75e72a02b3222
https://doi.org/10.1101/2022.12.23.521742

Host apoplastic cysteine protease activity is suppressed during the mutualistic association ofLolium perenneandEpichloë festucae

Autor: Barry Scott, Johana C. Misas Villamil, Kimberly A. Green, Fatih Demir, Andrea Passarge, Jasper R L Depotter, Pitter F. Huesgen, Gunther Doehlemann

Publikováno v: The journal of experimental botany 72(9), 3410–3426 (2021). doi:10.1093/jxb/erab088

Plants secrete various defence-related proteins into the apoplast, including proteases. Papain-like cysteine proteases (PLCPs) are central components of the plant immune system. To overcome plant immunity and successfully colonize their hosts, severa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::544dbda141cb0d247cdae8d5cb1ae3f3
https://doi.org/10.1093/jxb/erab088

Detection of Apoplastic Protease Inhibitors Using Convolution Activity-Based Protein Profiling

Autor: Andrea, Passarge, Gunther, Doehlemann, Johana C, Misas Villamil

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2447

Activity-based protein profiling (ABPP) is a powerful tool in biological chemistry to monitor protein activity using chemical probes that bind covalently and irreversible to active site of enzymes such as proteases. To date, there are three different

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::1661e038da780e79dc3493a1ea237652
https://pubmed.ncbi.nlm.nih.gov/35583775

A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif

Autor: André N. Mueller, Ute Meyer, Jan Schulze Hüynck, Marlen Breuer, Johana C. Misas Villamil, Joe Win, Pitter F. Huesgen, Gunther Doehlemann, Bilal Ökmen, Helen Dauben, Fatih Demir

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-15 (2019)
Nature Communications
Nature Communications 10(1), 1576 (2019). doi:10.1038/s41467-019-09472-8

Ustilago maydis is a biotrophic fungus causing corn smut disease in maize. The secreted effector protein Pit2 is an inhibitor of papain-like cysteine proteases (PLCPs) essential for virulence. Pit2 inhibitory function relies on a conserved 14 amino a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13e5dc3a651464c4feeda8f6c6672d03
http://link.springer.com/article/10.1038/s41467-019-09472-8