Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Johan H. van Heerden"'
Autor:
Pranas Grigaitis, Douwe A. J. Grundel, Eunice van Pelt-KleinJan, Mirushe Isaku, Guixiang Xie, Sebastian Mendoza Farias, Bas Teusink, Johan H. van Heerden
Publikováno v:
mSystems, Vol 7, Iss 4 (2022)
ABSTRACT The fission yeast, Schizosaccharomyces pombe, is a popular eukaryal model organism for cell division and cell cycle studies. With this extensive knowledge of its cell and molecular biology, S. pombe also holds promise for use in metabolism r
Externí odkaz:
https://doaj.org/article/ff808881b8cc4b5596f691d1f6a616d7
Autor:
Iraes Rabbers, Johan H. van Heerden, Niclas Nordholt, Herwig Bachmann, Bas Teusink, Frank J. Bruggeman
Publikováno v:
Metabolites, Vol 5, Iss 2, Pp 311-343 (2015)
Metabolism is generally required for cellular maintenance and for the generation of offspring under conditions that support growth. The rates, yields (efficiencies), adaptation time and robustness of metabolism are therefore key determinants of cellu
Externí odkaz:
https://doaj.org/article/1b19f713b4454d0a85967787826d8d82
Autor:
Johan H. van Heerden, Meike T. Wortel, Frank J. Bruggeman, Joseph J. Heijnen, Yves J.M. Bollen, Robert Planqué, Josephus Hulshof, Tom G. O’Toole, S. Aljoscha Wahl, Bas Teusink
Publikováno v:
Microbial Cell, Vol 1, Iss 3, Pp 103-106 (2015)
In the model eukaryote Saccharomyces cerevisiae, it has long been known that a functional trehalose pathway is indispensable for transitions to high glucose conditions. Upon addition of glucose, cells with a defect in trehalose 6-phosphate synthase (
Externí odkaz:
https://doaj.org/article/dd2ccf032fec4f26af023e85a24c081a
In many bacteria, translating ribosomes are excluded from the nucleoid, while amino-acid and energy-supplying metabolic enzymes spread evenly throughout the cytoplasm. Here we show with time-lapse fluorescence microscopy that this inhomogeneous organ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::89ec10006f8e0878a017f449c30fc18d
https://doi.org/10.1101/2023.04.18.537336
https://doi.org/10.1101/2023.04.18.537336
Autor:
Johan H. van Heerden, Cindy Iris Kranenburg, Dennis Botman, Daan Floris Boreel, Martijn Falk, Emile Zwering, Mariah B.M.J. Kes, Bas Teusink, Rinke J. van Tatenhove-Pel, Herwig Bachmann
Publikováno v:
van Tatenhove-Pel, R J, Zwering, E, Boreel, D F, Falk, M, van Heerden, J H, Kes, M B M J, Kranenburg, C I, Botman, D, Teusink, B & Bachmann, H 2021, ' Serial propagation in water-in-oil emulsions selects for Saccharomyces cerevisiae strains with a reduced cell size or an increased biomass yield on glucose ', Metabolic Engineering, vol. 64, pp. 1-14 . https://doi.org/10.1016/j.ymben.2020.12.005
Metabolic Engineering, 64, 1-14. Academic Press Inc.
Metabolic Engineering, 64, 1-14. Academic Press Inc.
In S. cerevisiae and many other micro-organisms an increase in metabolic efficiency (i.e. ATP yield on carbon) is accompanied by a decrease in growth rate. From a fundamental point of view, studying these yield-rate trade-offs provides insight in for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::58a1af0b586ae6cf969e897c7e370fc4
https://research.vu.nl/en/publications/c8136e5e-090a-4a38-af6b-f497871a0501
https://research.vu.nl/en/publications/c8136e5e-090a-4a38-af6b-f497871a0501
Autor:
Johan H. van Heerden, Frank J. Bruggeman, Tom O'Toole, Bas Teusink, Joachim Goedhart, Dennis Botman
Publikováno v:
Botman, D, O'Toole, T G, Goedhart, J, Bruggeman, F J, van Heerden, J H & Teusink, B 2021, ' A yeast FRET biosensor enlightens cAMP signaling ', Molecular Biology of the Cell, vol. 32, no. 13, pp. 1229-1240 . https://doi.org/10.1091/mbc.E20-05-0319
Molecular Biology of the Cell, 32(13), 1229-1240. American Society for Cell Biology
Botman, D, O'Toole, T G, Goedhart, J, Bruggeman, F J, van Heerden, J H & Teusink, B 2021, ' A yeast FRET biosensor enlightens cAMP signaling ', Molecular Biology of the Cell, vol. 32, no. 13, pp. 1229-1240 . https://doi.org/10.1091/MBC.E20-05-0319
Molecular Biology of the Cell
Molecular Biology of the Cell, 32(13), 1229-1240. American Society for Cell Biology
Botman, D, O'Toole, T G, Goedhart, J, Bruggeman, F J, van Heerden, J H & Teusink, B 2021, ' A yeast FRET biosensor enlightens cAMP signaling ', Molecular Biology of the Cell, vol. 32, no. 13, pp. 1229-1240 . https://doi.org/10.1091/MBC.E20-05-0319
Molecular Biology of the Cell
The cAMP-PKA signaling cascade in budding yeast regulates adaptation to changing environments. We developed yEPAC, a FRET-based biosensor for cAMP measurements in yeast. We used this sensor with flow cytometry for high-throughput single cell-level qu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0a82bb3ac28e6e1ab4e782f8cd829f9
https://research.vu.nl/en/publications/d4a8caea-3705-46f7-b976-73e0e5bce932
https://research.vu.nl/en/publications/d4a8caea-3705-46f7-b976-73e0e5bce932
Publikováno v:
Current Biology, 30(12), 2238-2247.e5. Cell Press
Nordholt, N, van Heerden, J H & Bruggeman, F J 2020, ' Biphasic Cell-Size and Growth-Rate Homeostasis by Single Bacillus subtilis Cells ', Current Biology, vol. 30, no. 12, pp. 2238-2247.e5 . https://doi.org/10.1016/j.cub.2020.04.030
Nordholt, N, van Heerden, J H & Bruggeman, F J 2020, ' Biphasic Cell-Size and Growth-Rate Homeostasis by Single Bacillus subtilis Cells ', Current Biology, vol. 30, no. 12, pp. 2238-2247.e5 . https://doi.org/10.1016/j.cub.2020.04.030
The growth rate of single bacterial cells is continuously disturbed by random fluctuations in biosynthesis rates and by deterministic cell-cycle events, such as division, genome duplication, and septum formation. It is not understood whether, and how
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f92bf69ecba828861750020b866b387b
https://research.vu.nl/en/publications/fedcb4ff-ec00-4f1b-9912-62ebb37a28ef
https://research.vu.nl/en/publications/fedcb4ff-ec00-4f1b-9912-62ebb37a28ef
Publikováno v:
ACS Sensors, 5(3), 814-822. American Chemical Society
ACS Sensors
Botman, D, Van Heerden, J H & Teusink, B 2020, ' An Improved ATP FRET Sensor for Yeast Shows Heterogeneity during Nutrient Transitions ', ACS Sensors, vol. 5, no. 3, pp. 814-822 . https://doi.org/10.1021/acssensors.9b02475
ACS Sensors
Botman, D, Van Heerden, J H & Teusink, B 2020, ' An Improved ATP FRET Sensor for Yeast Shows Heterogeneity during Nutrient Transitions ', ACS Sensors, vol. 5, no. 3, pp. 814-822 . https://doi.org/10.1021/acssensors.9b02475
Adenosine 5-triphosphate (ATP) is the main free energy carrier in metabolism. In budding yeast, shifts to glucose-rich conditions cause dynamic changes in ATP levels, but it is unclear how heterogeneous these dynamics are at the single-cell level. Fu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a17badf500ba81a43c827df08e0721d1
https://doi.org/10.1101/2019.12.12.874115
https://doi.org/10.1101/2019.12.12.874115
Autor:
Bas Teusink, Johan H. van Heerden, Joachim Goedhart, Dennis Botman, Tom O'Toole, Frank J. Bruggeman
The cAMP-PKA signalling cascade in budding yeast regulates adaptation to changing environments. We developed yEPAC, a FRET-based biosensor for cAMP measurements in yeast. We used this sensor with flow cytometry for high-throughput single cell-level q
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::93c5320738f03b60c8f8cdeb6f4b2e22
https://doi.org/10.1101/831354
https://doi.org/10.1101/831354
The growth rate of single bacterial cells is continuously disturbed by random fluctuations in biosynthesis rates and by deterministic cell-cycle events, such as division, genome duplication, and septum formation. It is not understood whether, and how
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2abb29104d8de0053fac9fda131e25d
https://doi.org/10.1101/510925
https://doi.org/10.1101/510925