Zobrazeno 1 - 10
of 36
pro vyhledávání: '"Johan Botterman"'
Autor:
Victor Klimyuk, Gerben van Eldik, Sylvestre Marillonnet, Anatoli Giritch, Yuri Gleba, Carola Engler, Johan Botterman
Publikováno v:
Proceedings of the National Academy of Sciences. 103:14701-14706
Plant viral vectors allow expression of heterologous proteins at high yields, but so far, they have been unable to express heterooligomeric proteins efficiently. We describe here a rapid and indefinitely scalable process for high-level expression of
Publikováno v:
Plant Physiology. 135:828-839
Enzymes that catalyze the condensation of acetyl coenzyme A and 2-oxo acids are likely to be important in two distinct metabolic pathways in Arabidopsis. These are the synthesis of isopropylmalate, an intermediate of Leu biosynthesis in primary metab
Autor:
Marc Van Montagu, Dirk Inzé, Tanya Moens, Luit Slooten, Jean-Marie Stassart, Frank Van Breusegem, Johan Botterman
Publikováno v:
Journal of Experimental Botany. 50:71-78
Transgenic maize plants have been generated by particle gun bombardment that overproduce a Nicotiana plumbaginifolia L. manganese superoxide dismutase (MnSOD). To target this mitochondrial enzyme into chloroplasts, the mature MnSOD-coding sequence wa
Autor:
Brigitte Chabbert, Johan Botterman, Marie Andrée Bernard-Vailhé, Marc Van Montagu, Chris Opsomer, Jean-Michel Besle, Marie Baucher
Publikováno v:
Plant Molecular Biology. 39:437-447
To improve the digestibility of the forage crop alfalfa (Medicago sativa L.), cinnamyl alcohol dehydrogenase (CAD), which catalyses the last step in the biosynthesis of the lignin monomers, was down-regulated by using an antisense approach. A subset
Autor:
Luit Slooten, Frank Van Breusegem, Dirk Inzé, Guy Bauw, Marc Van Montagu, Johan Botterman, Sergei Kushnir
Publikováno v:
Plant Molecular Biology. 38:491-496
Transgenic maize (Zea mays L.) and tobacco (Nicotiana tabacum Petit Havana SR1) plants have been generated, which overproduce a mitochondrial Nicotiana plumbaginifolia manganese superoxide dismutase (MnSOD) in chloroplasts. For this, the mature MnSOD
Publikováno v:
Bio/Technology. 13:1085-1089
We have tested a new strategy for engineering fungal disease resistance in crops which is based on inhibition of fungal growth and reproduction by controlled generation of necrotic lesions at infection sites, analogous to the naturally occurring hype
Autor:
Jef Seurinck, C. Simoens, Jeroen Coppieters, Luciana O Franco, Débora de Oliveira, M. Van Montagu, Johan Botterman
Publikováno v:
The Plant Journal. 3:495-507
Genomic and cDNA clones for three inflorescence-specific genes from Arabidopsis thaliana were isolated and characterized. The genes are tandemly organized in the genome on a 10 kb fragment. The expression of these genes is coordinately regulated in a
Publikováno v:
The EMBO Journal. 11:2345-2355
We studied protein sorting signals which are responsible for the retention of reticuloplasmins in the lumen of the plant endoplasmic reticulum (ER). A non-specific passenger protein, previously shown to be secreted by default, was used as a carrier f
Autor:
Kathleen D'halluin, Barbara Jean Mazur, Jan Leemans, Els Bonne, Johan Botterman, Martien Bossut
Publikováno v:
Nature Biotechnology. 10:309-314
We have developed an Agrobacterium mediated transformation procedure for sugarbeet, which is known for its recalcitrant behavior in vitro. A friable type of callus was used as the starting material, and combinations of different chimeric gene constru
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 483
Over the last decade, plant-based production of pharmaceuticals has made remarkable progress as the expression of a diverse set of proteins has been demonstrated in a range of plant crops. Although the commercial exploitation is still pending, today