Enhanced bioproduction of anticancer precursor vindoline by yeast cell factories

Autor: Natalja Kulagina, Grégory Guirimand, Céline Melin, Pamela Lemos‐Cruz, Ines Carqueijeiro, Johan‐Owen De Craene, Audrey Oudin, Vladimir Heredia, Konstantinos Koudounas, Marianne Unlubayir, Arnaud Lanoue, Nadine Imbault, Benoit St‐Pierre, Nicolas Papon, Marc Clastre, Nathalie Giglioli‐Guivarc’h, Jillian Marc, Sébastien Besseau, Vincent Courdavault

Publikováno v: Microbial Biotechnology, Vol 14, Iss 6, Pp 2693-2699 (2021)

Summary The pharmaceutical industry faces a growing demand and recurrent shortages in many anticancer plant drugs given their extensive use in human chemotherapy. Efficient alternative strategies of supply of these natural products such as bioproduct

Externí odkaz: https://doaj.org/article/60e784bff10b43a9a3cb622a92581120

Optimization of Tabersonine Methoxylation to Increase Vindoline Precursor Synthesis in Yeast Cell Factories

Autor: Pamela Lemos Cruz, Natalja Kulagina, Grégory Guirimand, Johan-Owen De Craene, Sébastien Besseau, Arnaud Lanoue, Audrey Oudin, Nathalie Giglioli-Guivarc’h, Nicolas Papon, Marc Clastre, Vincent Courdavault

Publikováno v: Molecules, Vol 26, Iss 12, p 3596 (2021)

Plant specialized metabolites are widely used in the pharmaceutical industry, including the monoterpene indole alkaloids (MIAs) vinblastine and vincristine, which both display anticancer activity. Both compounds can be obtained through the chemical c

Externí odkaz: https://doaj.org/article/9f981486c15348a2a55be115bff4d7c6

Study of the plant COPII vesicle coat subunits by functional complementation of yeast Saccharomyces cerevisiae mutants.

Autor: Johan-Owen De Craene, Fanny Courte, Bruno Rinaldi, Chantal Fitterer, Mari Carmen Herranz, Corinne Schmitt-Keichinger, Christophe Ritzenthaler, Sylvie Friant

Publikováno v: PLoS ONE, Vol 9, Iss 2, p e90072 (2014)

The formation and budding of endoplasmic reticulum ER-derived vesicles depends on the COPII coat protein complex that was first identified in yeast Saccharomyces cerevisiae. The ER-associated Sec12 and the Sar1 GTPase initiate the COPII coat formatio

Externí odkaz: https://doaj.org/article/3e04af6c28cb4da4aec7b8206d82d3da

Bi-Genomic Mitochondrial-Split-GFP – the yeast system for screening the mitochondrial matrix echoforms of dually localized proteins

Autor: Gaetan Bader, Ludovic Enkler, Yuhei Araiso, Marine Hemmerle, Krystyna Bińko, Emilia Baranowska, Aneta Więsyk, Johan-Owen De Craene, Julie Ruer-Laventie, Jean Pieters, Deborah Tribouillard-Tanvier, Bruno Senger, Jean-Paul di Rago, Sylvie Friant, Hubert Dominique Becker, Róża Kucharczyk

Publikováno v: Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1863:148895

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d93d0e213b8ea078c9940040d7fb4320
https://doi.org/10.1016/j.bbabio.2022.148895

Exploiting Spermidine N -Hydroxycinnamoyltransferase Diversity and Substrate Promiscuity to Produce Various Trihydroxycinnamoyl Spermidines and Analogues in Engineered Yeast

Autor: Thibaut Munsch, Natalja Kulagina, David Gagneul, Marianne Unlubayir, Benoit St-Pierre, Arnaud Lanoue, Thomas Dugé de Bernonville, Vincent Courdavault, Inês Carqueijeiro, Nathalie Giglioli-Guivarc’h, Johan-Owen De Craene, Jennifer Perrin, Marc Clastre, Sébastien Besseau

Publikováno v: ACS Synthetic Biology
ACS Synthetic Biology, American Chemical Society, 2021, 10 (2), pp.286-296. ⟨10.1021/acssynbio.0c00391⟩
ACS Synthetic Biology, 2021, 10 (2), pp.286-296. ⟨10.1021/acssynbio.0c00391⟩

International audience; Trihydroxycinnamoyl spermidines (THCSpd) are plant specialized metabolites with promising pharmacological activities as antifungals, antibacterial, antiviral, and antidepressant drugs. However, their characterization and poten

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c1c2ddc060fa278825decfd4c165cf9
https://hal-univ-tours.archives-ouvertes.fr/hal-03287505