Zobrazeno 1 - 10
of 35
pro vyhledávání: '"Joel Osuna"'
Autor:
Edson Cárcamo, Abigail Roldán-Salgado, Joel Osuna, Iván Bello-Sanmartin, Jorge A. Yáñez, Gloria Saab-Rincón, Héctor Viadiu, Paul Gaytán
Publikováno v:
ACS Omega, Vol 2, Iss 7, Pp 3183-3191 (2017)
Externí odkaz:
https://doaj.org/article/5c1ab5ceec4548d69256376c479fe82c
Publikováno v:
Archives of biochemistry and biophysics. 714
EF-hand is a common motif in Ca
Publikováno v:
Archives of Biochemistry and Biophysics. 716:109108
Publikováno v:
Archives of Biochemistry and Biophysics. 714:109065
EF-hand is a common motif in Ca2+-binding proteins, some of which present a conformational change upon Ca2+-binding, a relevant property for signal transduction. In the present work, we investigated the behavior of Calbindin D9k, a modulator protein
Publikováno v:
Microbial Physiology. 27:1-10
Recombinant protein expression is one of the key issues in protein engineering and biotechnology. Among the different models for assessing protein production and structure-function studies, green fluorescent protein (GFP) is one of the preferred mode
Autor:
Jorge Sánchez, Sabino Pacheco, Isabel Gómez, Mary Carmen Torres-Quintero, Humberto Flores, Gretel Mendoza, Joel Osuna, Alejandra Bravo, Mario Soberón
Publikováno v:
Scientific Reports
Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
The Cyt and Cry toxins are different pore-forming proteins produced by Bacillus thuringiensis bacteria, and used in insect-pests control. Cry-toxins have a complex mechanism involving interaction with several proteins in the insect gut such as aminop
Autor:
Jorge González-Bacerio, Yanira Méndez, María A. Chávez, Rafael Fando, Jean-Louis Charli, Katherine Figarella, Joel Osuna, Amaia Ponce
Publikováno v:
Protein Expression and Purification. 104:103-114
Plasmodium falciparum neutral metallo-aminopeptidase (PfAM1), a member of the M1 family of metallo proteases, is a promising target for malaria, a devastating human parasitic disease. We report the high-level expression of PfAM1 in Escherichia coli B
Autor:
Jorge Yáñez, Hector Viadiu, Gloria Saab-Rincón, Abigail Roldán-Salgado, Iván Bello-Sanmartin, Joel Osuna, Edson Cárcamo, Paul Gaytán
Publikováno v:
ACS Omega
ACS Omega, Vol 2, Iss 7, Pp 3183-3191 (2017)
ACS Omega, Vol 2, Iss 7, Pp 3183-3191 (2017)
In vitro mutagenesis methods have revolutionized biological research and the biotechnology industry. In this study, we describe a mutagenesis method based on synthesizing a gene using a complete set of forward and reverse spiked oligonucleotides that
Autor:
Adrián Pérez-Maldonado, Benito Barrera, Joel Osuna, Jean-Louis Charli, Patricia Joseph-Bravo, Antonieta Cote-Vélez
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms. 1809:191-199
Expression of hypophysiotropic TRH, that controls thyroid axis activity, is increased by cold exposure; this effect is mimicked in rat hypothalamic cells incubated with norepinephrine or cAMP analogs. TRH proximal promoter contains three putative CRE
Autor:
Agustín López-Munguía, Xavier Soberón, Alfredo Martinez, Joel Vega-Badillo, Joel Osuna, Gabriela Espinosa-Molina, Guillermo Gosset, Consuelo Vazquez-Limón
Publikováno v:
Biotechnology Letters. 29:1857-1863
By complementing a non-fermentative Escherichia coli (ldhA (-) pflB (-)) strain with the recombinant Zymomonas mobilis ethanol pathway (pdc, adhB), we evaluated the effect of different levels of enzymatic activity on growth rate demonstrating that th