Rational correction of pathogenic conformational defects in HTRA1

Autor: Nathalie Beaufort, Linda Ingendahl, Melisa Merdanovic, Andree Schmidt, David Podlesainski, Tim Richter, Thorben Neumann, Michael Kuszner, Ingrid R. Vetter, Patricia Stege, Steven G. Burston, Anto Filipovic, Yasser B. Ruiz-Blanco, Kenny Bravo-Rodriguez, Joel Mieres-Perez, Christine Beuck, Stephan Uebel, Monika Zobawa, Jasmin Schillinger, Rainer Malik, Katalin Todorov-Völgyi, Juliana Rey, Annabell Roberti, Birte Hagemeier, Benedikt Wefers, Stephan A. Müller, Wolfgang Wurst, Elsa Sanchez-Garcia, Alexander Zimmermann, Xiao-Yu Hu, Tim Clausen, Robert Huber, Stefan F. Lichtenthaler, Carsten Schmuck, Michael Giese, Markus Kaiser, Michael Ehrmann, Martin Dichgans

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-18 (2024)

Abstract Loss-of-function mutations in the homotrimeric serine protease HTRA1 cause cerebral vasculopathy. Here, we establish independent approaches to achieve the functional correction of trimer assembly defects. Focusing on the prototypical R274Q m

Externí odkaz: https://doaj.org/article/ecefd6f18eff4edf90c7e557870791d5

Specific inhibition of the Survivin–CRM1 interaction by peptide-modified molecular tweezers

Autor: Annika Meiners, Sandra Bäcker, Inesa Hadrović, Christian Heid, Christine Beuck, Yasser B. Ruiz-Blanco, Joel Mieres-Perez, Marius Pörschke, Jean-Noël Grad, Cecilia Vallet, Daniel Hoffmann, Peter Bayer, Elsa Sánchez-García, Thomas Schrader, Shirley K. Knauer

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)

Survivin’s dual function as apoptosis inhibitor and regulator of cell proliferation is mediated via its interaction with the export receptor CRM1. Here authors report a strategy addressing its dimer interface overlapping with the nuclear export sig

Externí odkaz: https://doaj.org/article/57a8962dc1a04df6ae79162b6fd9c226

PPI-Affinity: A Web Tool for the Prediction and Optimization of Protein-Peptide and Protein-Protein Binding Affinity

Autor: Sandra Romero-Molina, Yasser B. Ruiz-Blanco, Joel Mieres-Perez, Mirja Harms, Jan Münch, Michael Ehrmann, Elsa Sanchez-Garcia

Publikováno v: Journal of proteome research. 21(8)

Virtual screening of protein-protein and protein-peptide interactions is a challenging task that directly impacts the processes of hit identification and hit-to-lead optimization in drug design projects involving peptide-based pharmaceuticals. Althou

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd3165a25f47be3f9940fe404dcc2e61
https://pubmed.ncbi.nlm.nih.gov/35654412

An allosteric HTRA1-calpain 2 complex with restricted activation profile

Autor: Juliana Rey, Maike Breiden, Vanda Lux, Anika Bluemke, Maike Steindel, Kamilla Ripkens, Bastian Möllers, Kenny Bravo Rodriguez, Prisca Boisguerin, Rudolf Volkmer, Joel Mieres-Perez, Tim Clausen, Elsa Sanchez-Garcia, Michael Ehrmann

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, 2022, 119 (14), pp.e2113520119. ⟨10.1073/pnas.2113520119⟩

Significance Classic serine proteases are synthesized as inactive precursors that are proteolytically processed, resulting in irreversible activation. We report an alternative and reversible mechanism of activation that is executed by an inactive pro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21bffd9bbaeb049d992fcf1c102e5723
https://hal.science/hal-03625904

Advanced Molecular Tweezers with Lipid Anchors against SARS-CoV-2 and Other Respiratory Viruses

Autor: Tatjana Weil, Abbna Kirupakaran, My-Hue Le, Philipp Rebmann, Joel Mieres-Perez, Leila Issmail, Carina Conzelmann, Janis A. Müller, Lena Rauch, Andrea Gilg, Lukas Wettstein, Rüdiger Groß, Clarissa Read, Tim Bergner, Sandra Axberg Pålsson, Nadja Uhlig, Valentina Eberlein, Heike Wöll, Frank-Gerrit Klärner, Steffen Stenger, Beate M. Kümmerer, Hendrik Streeck, Giorgio Fois, Manfred Frick, Peter Braubach, Anna-Lena Spetz, Thomas Grunwald, James Shorter, Elsa Sanchez-Garcia, Thomas Schrader, Jan Münch

Publikováno v: JACS Au

The COVID-19 pandemic caused by SARS-CoV-2 presents a global health emergency. Therapeutic options against SARS-CoV-2 are still very limited but urgently required. Molecular tweezers are supramolecular agents that destabilize the envelope of viruses

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05e23a0fa6926dc7b1800439d47a6417