Zobrazeno 1 - 10
of 37
pro vyhledávání: '"Joel A Lawitts"'
Publikováno v:
PLoS ONE, Vol 9, Iss 6, p e99809 (2014)
The purpose of this study is to develop a mouse sperm preservation method based on evaporative drying. Mouse sperm were evaporatively dried and stored at 4°C and ambient temperature for 3 months to 2 years. Upon rehydration, a single sperm was injec
Externí odkaz:
https://doaj.org/article/d7fe4e1f335847248b103366c065b6ea
Publikováno v:
PLoS ONE, Vol 7, Iss 1, p e29924 (2012)
With the fast advancement in the genetics and bio-medical fields, the vast number of valuable transgenic and rare genetic mouse models need to be preserved. Preservation of mouse sperm by convective drying and subsequent storing at above freezing tem
Externí odkaz:
https://doaj.org/article/c58afff4d3c744fcbd972cc0a318a482
Publikováno v:
Journal of Animal Science. 90:3739-3742
A piezo-driven pipette that includes a small amount of mercury to enhance efficiency is widely used for mouse intracytoplasmic sperm injection (ICSI). Unfortunately, the use of toxic mercury is not permitted in hospital facilities and alternatives to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::508dff9c78b1bd467b61a68ca069fbc4
https://doi.org/10.2527/jas.2012-5136
https://doi.org/10.2527/jas.2012-5136
Autor:
Lynda K. McGinnis, Mehmet Toner, Sankha Bhowmick, John D. Biggers, Liben Zhu, Joel A. Lawitts
Publikováno v:
Biology of Reproduction. 73:627-633
Mouse sperm with and without trehalose were desiccated under nitrogen gas and stored at 4°C and 22°C. After rehydration, sperm were injected into oocytes using intracytoplasmic sperm injection and embryonic development was followed. Sperm were drie
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1664379718eafd99f79ce99d8fdb5585
https://doi.org/10.1095/biolreprod.105.042291
https://doi.org/10.1095/biolreprod.105.042291
Autor:
Bharat D. Nath, Liben Zhu, John D. Biggers, Mehmet Toner, Lynda K. McGinnis, Sankha Bhowmick, Joel A. Lawitts
Publikováno v:
Biology of Reproduction. 68:1779-1786
Long-term preservation of mouse sperm by desiccation is economically and logistically attractive. The current investigation is a feasibility study of the preservation of mouse sperm by convective drying in an inert gas (nitrogen). Mouse sperm from th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::02262f5b31117fde3eaecac4b903a584
https://doi.org/10.1095/biolreprod.102.009407
https://doi.org/10.1095/biolreprod.102.009407
Autor:
Xiaolian Tan, Janice A. Nagy, Dian Feng, Eliza Vasile, Ann M. Dvorak, Christian Sundberg, Harold F. Dvorak, Michael Detmar, Joel A. Lawitts, Laura E. Benjamin, Eleanor J. Manseau, Lawrence F. Brown
Publikováno v:
The Journal of Experimental Medicine
Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF, VEGF-A) is a multifunctional cytokine with important roles in pathological angiogenesis. Using an adenoviral vector engineered to express murine VEGF-A164, we previously inves
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b2020c088e7c43e1cd7d5459b80dec21
http://europepmc.org/articles/PMC2194262
http://europepmc.org/articles/PMC2194262
Publikováno v:
PLoS ONE
PLoS ONE, Vol 9, Iss 6, p e99809 (2014)
PLoS ONE, Vol 9, Iss 6, p e99809 (2014)
The purpose of this study is to develop a mouse sperm preservation method based on evaporative drying. Mouse sperm were evaporatively dried and stored at 4°C and ambient temperature for 3 months to 2 years. Upon rehydration, a single sperm was injec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76254687bb0b33fdf046149790213a96
https://pubmed.ncbi.nlm.nih.gov/24924588
https://pubmed.ncbi.nlm.nih.gov/24924588
Autor:
Pamela L. Sheridan, Konstantin Khrapko, Maxwell C. Furr, Joel A. Lawitts, Xiaomin Zhang, Ana M. Borras, Jianyuan Chai, Ravi P. Misra, Gohar Azhar, Jeanne Y. Wei
Publikováno v:
Journal of Biological Chemistry. 276:40033-40040
Serum response factor (SRF) is a key regulator of a number of extracellular signal-regulated genes important for cell growth and differentiation. A form of the SRF gene with a double mutation (dmSRF) was generated. This mutation reduced the binding a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec61bb759579dfadec28f841e4764071
https://doi.org/10.1074/jbc.m104934200
https://doi.org/10.1074/jbc.m104934200
Autor:
Colin T. Maguire, David L. Paul, Hiroko Wakimoto, Warren J. Manning, Weei-Yuarn Huang, Hideko Kasahara, Tetsuo Shioi, Charles I. Berul, Joel A. Lawitts, Margaret Liu, Seigo Izumo, Kimber L. Converso
Publikováno v:
Journal of Clinical Investigation. 108:189-201
A DNA nonbinding mutant of the NK2 class homeoprotein Nkx2.5 dominantly inhibits cardiogenesis in Xenopus embryos, causing a small heart to develop or blocking heart formation entirely. Recently, ten heterozygous CSX/NKX2.5 homeoprotein mutations wer
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d32b318791f59d541acb611081dc8fd4
https://doi.org/10.1172/jci12694
https://doi.org/10.1172/jci12694
Autor:
Jihong Yang, Jeanne Y. Wei, Gohar Azhar, Koichiro Nagano, Joel A. Lawitts, Jianyuan Chai, Xiaomin Zhang, Pamela L. Sheridan, Ana M. Borras, Thomas Brown, Konstantin Khrapko, Ravi P. Misra
Publikováno v:
American Journal of Physiology-Heart and Circulatory Physiology. 280:H1782-H1792
Serum response factor (SRF), a member of the MCM1, agamous, deficiens, SRF (MADS) family of transcriptional activators, has been implicated in the transcriptional control of a number of cardiac muscle genes, including cardiac alpha-actin, skeletal al
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dba46b6c7213700f3aca45971846e8cb
https://doi.org/10.1152/ajpheart.2001.280.4.h1782
https://doi.org/10.1152/ajpheart.2001.280.4.h1782