Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Joe Uknalis"'
Publikováno v:
Sensors, Vol 6, Iss 8, Pp 796-807 (2006)
Fiber optic biosensor has a great potential to meet the need for rapid, sensitive,and real-time microbial detection systems. We developed an antibody-based fiber-opticbiosensor to rapidly detect low levels of Escherichia coli O157:H7 cells in ground
Externí odkaz:
https://doaj.org/article/c4a34a9fdd0f47b8a6138229ec5f0256
Publikováno v:
Compost Science & Utilization. 22:253-262
On farm production of arbuscular mycorrhizal (AM) fungi is suitable for vegetable and horticultural crop production because the inocula may be efficiently mixed into horticultural potting media for plant production in the greenhouse. These inocula ar
Publikováno v:
Journal of Rapid Methods and Automation in Microbiology. 13:57-70
Escherichia coli O157:H7 was inoculated into ground beef. Samples were incubated in E. coli broth plus novobiocin growth media at 37C for 5 h. Bacteria were captured by magnetic beads coated with anti-E. coli O157:H7 antibodies and were treated with
Publikováno v:
Journal of Rapid Methods & Automation in Microbiology. 11:35-46
Biotinylated anti E. coli O157 antibodies were linked to streptavidin coated magnetic and polystyrene beads of different sizes and densities. These beads were then used to capture cultured E. coli O157:H7. Complexes formed between fluorescein-conjuga
Publikováno v:
Journal of Rapid Methods and Automation in Microbiology. 10:185-195
Two types of commercially available, streptavidin-coated magnetic beads (SAMB) were labeled with the same biotinylated anti E. coli O157 antibodies. Labeled SAMB and other necessary antibody derivatives needed for Light-addressable Potentiometric Sen
Publikováno v:
Food Research International. 33:375-380
A procedure for rapid assay (about 30 min) of viable Escherichia coli O157:H7 in solutions is described. The bacteria, captured by specific immunomagnetic beads, were lysed by commercially available reagents. The released cellular ATP was determined
Publikováno v:
Journal of Food Safety. 18:113-126
Live cells of E. coli 0157:H7 were labeled by 4',6 -diamidino-2-phenylindole (DAPI) in buffers of different pH. The extent of labeling was relatively insensitive to pH in the range of 6.5 to 9.5. The fluorescence intensity of ∼ 10 4 DAPI-labeled ba
Publikováno v:
Protoplasma. 192:70-79
Fluorescence microscopy offers some distinct advantages over other techniques for studying ion transport processes in situ with plant cells. However, the use of this technology in plant cells has been limited by our lack of understanding the mechanis
Publikováno v:
SPIE Proceedings.
We developed an antibody-based fiber-optic biosensor to rapidly detect low levels of Escherichia coli O157:H7 and shiga-like toxins (SLTs) in ground beef samples. The principle of the sensor is a sandwich immunoassay using an antibody which is specif
Publikováno v:
Biosensorsbioelectronics. 22(7)
Piezoelectric-excited millimeter-sized cantilever (PEMC) sensors consisting of a piezoelectric and a borosilicate glass layer with a sensing area of 4 mm2 were fabricated. An antibody specific to Escherichia coli (anti-E. coli) O157:H7 was immobilize