Výsledky vyhledávání - "Jochen Deckert"

Hepatocyte-targeted RNAi Therapeutics for the Treatment of Chronic Hepatitis B Virus Infection

Autor: Darren H. Wakefield, Kerstin Jahn-Hofmann, Julia Hegge, Claudia E. Oropeza, Hans-Peter Vornlocher, Markus Hossbach, Qili Chu, David B. Rozema, Ingo Roehl, Alan McLachlan, Holly Hamilton, Philipp Hadwiger, Christine I. Wooddell, Matthias John, Jason Klein, Jochen Deckert, David L. Lewis

Publikováno v: Molecular Therapy

RNA interference (RNAi)-based therapeutics have the potential to treat chronic hepatitis B virus (HBV) infection in a fundamentally different manner than current therapies. Using RNAi, it is possible to knock down expression of viral RNAs including t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd54760eecf60d79cd46602fcf7f0c3a

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Hepatitis B virus genome replication triggers toll-like receptor 3-dependent interferon responses in the absence of hepatitis B surface antigen

Autor: Ruth Broering, Kerstin Jahn-Hofmann, Ulf Dittmer, Matthias John, Jochen Deckert, Kathrin Gibbert, Reinhold Schirmbeck, CI Real, L Ickenstein, Hans-Peter Vornlocher, Mengji Lu, Xuan Huang, Guido Gerken, Joerg F. Schlaak, Martin Trippler, Jia Liu, Markus Hossbach

Publikováno v: Scientific Reports

The hepatitis B virus (HBV) has been described as stealth virus subverting immune responses initially upon infection. Impaired toll-like receptor signaling by the HBV surface antigen (HBsAg) attenuates immune responses to facilitate chronic infection

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87a3c9bee88b7cbd1f2700f0bb4b97ca

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Semiquantitative Proteomic Analysis of the Human Spliceosome via a Novel Two-Dimensional Gel Electrophoresis Method

Autor: Sergey Bessonov, Cindy L. Will, Elmar Wolf, Dmitry E. Agafonov, Reinhard Lührmann, Henning Urlaub, Jochen Deckert, Peter Odenwälder

Publikováno v: Molecular and Cellular Biology

More than 200 proteins associate with human spliceosomes, but little is known about their relative abundances in a given spliceosomal complex. Here we describe a novel two-dimensional (2D) electrophoresis method that allows separation of high-molecul

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9fdcf6099562d79c907b72b4406a9a3a
https://doi.org/10.1128/mcb.05266-11

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Composition and three-dimensional EM structure of double affinity-purified, human prespliceosomal A complexes

Autor: Klaus Hartmuth, Monika M. Golas, Bjoern Sander, Holger Stark, Berthold Kastner, Prakash Dube, Nastaran Behzadnia, Henning Urlaub, Cindy L. Will, Jochen Deckert, Reinhard Lührmann

Publikováno v: The EMBO Journal

Little is known about the higher-order structure of prespliceosomal A complexes, in which pairing of the pre-mRNA's splice sites occurs. Here, human A complexes were isolated under physiological conditions by double-affinity selection. Purified compl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bcadd26ec1b297908b6707a4dd91f16
https://doi.org/10.1038/sj.emboj.7601631

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U2 snRNA-Protein Contacts in Purified Human 17S U2 snRNPs and in Spliceosomal A and B Complexes

Autor: Olexandr Dybkov, Cindy L. Will, Reinhard Lührmann, Jochen Deckert, Klaus Hartmuth, Nastaran Behzadnia

Publikováno v: Molecular and Cellular Biology

The 17S U2 snRNP plays an essential role in branch point selection and catalysis during pre-mRNA splicing. Much remains to be learned about the molecular architecture of the U2 snRNP, including which proteins contact the functionally important 5′ e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35c5dc1d311bbfa1bc9a223fbfb79ad0
https://doi.org/10.1128/mcb.26.7.2803-2816.2006

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Mass spectrometry-based relative quantification of proteins in precatalytic and catalytically active spliceosomes by metabolic labeling (SILAC), chemical labeling (iTRAQ), and label-free spectral count

Autor: Mads Grønborg, Sergey Bessonov, Henning Urlaub, Jochen Deckert, Thomas Conrad, Reinhard Lührmann, Carla Schmidt

Publikováno v: RNA

The spliceosome undergoes major changes in protein and RNA composition during pre-mRNA splicing. Knowing the proteins—and their respective quantities—at each spliceosomal assembly stage is critical for understanding the molecular mechanisms and r

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca0674ffcea15dd07f8eb17f06d42b71
https://pubmed.ncbi.nlm.nih.gov/24448447

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In vivo expression and purification of aptamer-tagged small RNA regulators

Autor: Henning Urlaub, Jörg Vogel, Robert Hurwitz, Jochen Deckert, Renate Rieder, Nelly Said

Publikováno v: Nucleic Acids Research

Small non-coding RNAs (sRNAs) are an emerging class of post-transcriptional regulators of bacterial gene expression. To study sRNAs and their potential protein interaction partners, it is desirable to purify sRNAs from cells in their native form. Her

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5e1a51eea1a9266636a96754c2a1dca
https://pubmed.ncbi.nlm.nih.gov/19726584

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Exon, intron and splice site locations in the spliceosomal B complex

Autor: Holger Stark, Christian Merz, Elmar Wolf, Jochen Deckert, Reinhard Lührmann, Berthold Kastner

Publikováno v: EMBO Journal

In recent years, electron microscopy (EM) has allowed the generation of three-dimensional structure maps of several spliceosomal complexes. However, owing to their limited resolution, little is known at present about the location of the pre-mRNA, the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e6e31fe0292e005d3308e8f377572ea7
http://www.nature.com/emboj/journal/v28/n15/pdf/emboj2009171a.pdf

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