Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Jocelyn Charlton"'
Autor:
Christina Galonska, Jocelyn Charlton, Alexandra L. Mattei, Julie Donaghey, Kendell Clement, Hongcang Gu, Arman W. Mohammad, Elena K. Stamenova, Davide Cacchiarelli, Sven Klages, Bernd Timmermann, Tobias Cantz, Hans R. Schöler, Andreas Gnirke, Michael J. Ziller, Alexander Meissner
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
Catalytically inactive Cas9 fused to a methyltransferase has emerged as a promising epigenome modifying tool. Here the authors generate a methylation depleted but maintenance competent mouse ES cell line and find ubiquitous off-target activity.
Externí odkaz:
https://doaj.org/article/facfcb998fb94a91ab5dd5199cd3a1b4
Autor:
Yingying Zhang, Jocelyn Charlton, Rahul Karnik, Isabel Beerman, Zachary D Smith, Hongcang Gu, Patrick Boyle, Xiaoli Mi, Kendell Clement, Ramona Pop, Andreas Gnirke, Derrick J Rossi, Alexander Meissner
Publikováno v:
eLife, Vol 7 (2018)
DNA methylation plays an essential role in mammalian genomes and expression of the responsible enzymes is tightly controlled. Deregulation of the de novo DNA methyltransferase DNMT3B is frequently observed across cancer types, yet little is known abo
Externí odkaz:
https://doaj.org/article/ec968c14f5da4542813e3bd8668faa0a
Autor:
Douglas A. Melton, Alexander Meissner, Niloofar Rasouli, Juerg R. Straubhaar, Jennifer H. R. Kenty, Julie Donaghey, Juan R. Alvarez-Dominguez, Aharon Helman, Jocelyn Charlton
Publikováno v:
StemJournal. 2:7-13
Stem cell-derived tissues that recap endogenous physiology are key for regenerative medicine. Yet, most methods yield products that function like fetal, not adult tissues. Organoids are typically grown in constant environments, while our tissues matu
Netrin-1 promotes naive pluripotency through Neo1 and Unc5b co-regulation of Wnt and MAPK signalling
Autor:
Alexander Meissner, Manuel Koch, Jenny Y. Zhang, Nicolas Gadot, Xavier Gaume, Fabrice Lavial, Nicolas Rama, Marie Brevet, Pauline Wajda, Thomas Imhof, Bradley J. Merrill, Noémie Combémorel, Jocelyn Charlton, Christina Riemenschneider, Nicolas Allègre, Isabelle Durand, Patrick Mehlen, Claire Chazaud, Pauline Vieugué, Christina Galonska, Duygu Ozmadenci, Giacomo Furlan, Aurélia Huyghe
Publikováno v:
Nature Cell Biology
Nature Cell Biology, 2020, 22 (4), pp.389-400. ⟨10.1038/s41556-020-0483-2⟩
Nature Cell Biology, Nature Publishing Group, 2020, 22 (4), pp.389-400. ⟨10.1038/s41556-020-0483-2⟩
Nature Cell Biology, 2020, 22 (4), pp.389-400. ⟨10.1038/s41556-020-0483-2⟩
Nature Cell Biology, Nature Publishing Group, 2020, 22 (4), pp.389-400. ⟨10.1038/s41556-020-0483-2⟩
International audience; In mouse embryonic stem cells (mESCs), chemical blockade of Gsk3α/β and Mek1/2 (2i) instructs a self-renewing ground state whose endogenous inducers are unknown. Here we show that the axon guidance cue Netrin-1 promotes naiv
Autor:
Simona Cristea, Fei Ji, Michael S. Hoetker, Bruno Di Stefano, Ruslan I. Sadreyev, Masakazu Yamamoto, Kathleen A. Messemer, Alexander Meissner, Aaron J. Huebner, Franziska Michor, Masaki Yagi, Naftali Horwitz, Anthony Patelunas, David J. Goldhamer, A. Almada, Nikolaos Tsopoulidis, Konrad Hochedlinger, Jocelyn Charlton, Ori Bar-Nur, Amy J. Wagers
Publikováno v:
Genes Dev
Genes and Development
Genes & Development, 35 (17-18)
Genes and Development
Genes & Development, 35 (17-18)
The generation of myotubes from fibroblasts upon forced MyoD expression is a classic example of transcription factor-induced reprogramming. We recently discovered that additional modulation of signaling pathways with small molecules facilitates repro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::761430d06c8b0d8285b6cc1b0b333fe2
Autor:
Stuart Aitken, Viktoriya Stancheva, Jocelyn Charlton, Nicholas D. Hastie, Ruthrothaselvi Bharathavikru, Joan Slight, Giulia Petrovich, Kathy Pritchard-Jones, Abdelkader Essafi
Wilms’ tumour 1 (WT1) is a transcription factor and a tumour suppressor, essential for the development and homeostasis of multiple tissues derived from the intermediate and lateral plate mesoderm. Germline WT1 mutations result in the eponymous paed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83d4169bdec49dea9fc4c1b654be537c
Autor:
Kendell Clement, Hans R. Schöler, Alexandra L. Mattei, Elena K. Stamenova, Julie Donaghey, Andreas Gnirke, Hongcang Gu, Alexander Meissner, Bernd Timmermann, Christina Galonska, Arman W. Mohammad, Jocelyn Charlton, Tobias Cantz, Sven Klages, Michael J. Ziller, Davide Cacchiarelli
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
Nature Communications
Nature Communications
In normal mammalian development cytosine methylation is essential and is directed to specific regions of the genome. Despite notable advances through mapping its genome-wide distribution, studying the direct contribution of DNA methylation to gene an
Autor:
Julie Donaghey, Kendell Clement, Jennifer S. Chen, Andreas Gnirke, Casey A. Gifford, John L. Rinn, Michael J. Ziller, Ramona Pop, Rahul Karnik, Elena K. Stamenova, Jocelyn Charlton, David R. Kelley, Sudhir Thakurela, Hongcang Gu, Alexander Meissner, Zachary D. Smith, Davide Cacchiarelli
Publikováno v:
Nature Genetics
Transcription factors (TFs) direct developmental transitions by binding to target DNA sequences, influencing gene expression and establishing complex gene-regultory networks. To systematically determine the molecular components that enable or constra
Autor:
Kendell Clement, Chuck Haggerty, Stefan Aigner, Hongcang Gu, Alexander Meissner, Inna Lipchina, Anthony Anselmo, Steven P. Gygi, Justin Brumbaugh, Fei Ji, John L. Pulice, Mattia F. M. Gerli, Katie J. Clowers, Aaron J. Huebner, Inés Rabano Jiménez, En-Ching Luo, Ruslan I. Sadreyev, Bruno Di Stefano, Konrad Hochedlinger, Jocelyn Charlton, Marit A.C. de Kort, Gene W. Yeo
Publikováno v:
Cell Stem Cell
Cell stem cell, vol 25, iss 5
Cell stem cell, vol 25, iss 5
Post-transcriptional mechanisms have the potential to influence complex changes in gene expression, yet their role in cell fate transitions remains largely unexplored. Here, we show that suppression of the RNA helicase DDX6 endows human and mouse pri
Autor:
Nina Bailly, Andreas Gnirke, Alexander Meissner, Elena K. Stamenova, Eric J. Martin, Franz-Josef Müller, Jocelyn Charlton, Björn Brändl, Zachary D. Smith, Alexandra L. Mattei, Evangelos Kiskinis, Jing Liao, Pay Giesselmann, Eunmi J. Jung
Publikováno v:
Nature genetics
Nature Genetics
Nature Genetics
Mammalian cells stably maintain high levels of DNA methylation despite expressing both positive (DNMT3A/B) and negative (TET1–3) regulators. Here, we used wildtype and TET triple knockout human embryonic stem cells (ESCs), generated DNMT3-null as w