Fluorescence-Based Methods for the Study of Biomolecular Interactions.

Autor: Jobin ML; Interdisciplinary Institute for Neurosciences (IINS), CNRS UMR5297, University of Bordeaux; marie-lise.jobin@u-bordeaux.fr.

Publikováno v: Journal of visualized experiments : JoVE [J Vis Exp] 2024 Feb 23 (204). Date of Electronic Publication: 2024 Feb 23.

Correction: Impact of membrane lipid polyunsaturation on dopamine D2 receptor ligand binding and signaling.

Autor: Jobin ML; Université de Bordeaux, INRAE, Bordeaux INP, NutriNeuro, 33000, Bordeaux, France., De Smedt-Peyrusse V; Université de Bordeaux, INRAE, Bordeaux INP, NutriNeuro, 33000, Bordeaux, France., Ducrocq F; Université de Bordeaux, INRAE, Bordeaux INP, NutriNeuro, 33000, Bordeaux, France., Baccouch R; Institute of Chemistry & Biology of Membranes & Nanoobjects, CNRS UMR 5248, Université de Bordeaux, Bordeaux INP, 33600, Pessac, France., Oummadi A; Université de Bordeaux, INRAE, Bordeaux INP, NutriNeuro, 33000, Bordeaux, France., Pedersen MH; Department of Psychiatry, Columbia University Vagelos College of Physicians and Surgeons, New York, NY, 10032, USA.; Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, NY, 10032, USA., Medel-Lacruz B; Research Programme on Biomedical Informatics (GRIB), Hospital del Mar Medical Research Institute (IMIM)-Department of Experimental and Health Sciences, Pompeu Fabra University (UPF), 08003, Barcelona, Spain., Angelo MF; Université de Bordeaux, INRAE, Bordeaux INP, NutriNeuro, 33000, Bordeaux, France., Villette S; Institute of Chemistry & Biology of Membranes & Nanoobjects, CNRS UMR 5248, Université de Bordeaux, Bordeaux INP, 33600, Pessac, France., Van Delft P; Laboratory of Membrane Biogenesis (LBM), Research Mix Unity (UMR) 5200, National Scientific Research Center (CNRS), University of Bordeaux, Bordeaux, France., Fouillen L; Laboratory of Membrane Biogenesis (LBM), Research Mix Unity (UMR) 5200, National Scientific Research Center (CNRS), University of Bordeaux, Bordeaux, France., Mongrand S; Laboratory of Membrane Biogenesis (LBM), Research Mix Unity (UMR) 5200, National Scientific Research Center (CNRS), University of Bordeaux, Bordeaux, France., Selent J; Research Programme on Biomedical Informatics (GRIB), Hospital del Mar Medical Research Institute (IMIM)-Department of Experimental and Health Sciences, Pompeu Fabra University (UPF), 08003, Barcelona, Spain., Tolentino-Cortez T; Research Department, IMG Pharma Biotech S.L., BIC Bizkaia (612), 48160, Derio, Spain., Barreda-Gómez G; Research Department, IMG Pharma Biotech S.L., BIC Bizkaia (612), 48160, Derio, Spain., Grégoire S; Centre des Sciences du Goût et de l'Alimentation, AgroSup Dijon, CNRS, INRAE, Université Bourgogne Franche-Comté, 21000, Dijon, France., Masson E; Centre des Sciences du Goût et de l'Alimentation, AgroSup Dijon, CNRS, INRAE, Université Bourgogne Franche-Comté, 21000, Dijon, France., Durroux T; Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, Montpellier, France., Javitch JA; Department of Psychiatry, Columbia University Vagelos College of Physicians and Surgeons, New York, NY, 10032, USA.; Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, NY, 10032, USA.; Department of Molecular Pharmacology and Therapeutics, Columbia University Vagelos College of Physicians and Surgeons, New York, NY, 10032, USA., Guixà-González R; Condensed Matter Theory Group, Paul Scherrer Institute (PSI), 5232, Villigen, PSI, Switzerland. ramon.guixa@psi.ch., Alves ID; Institute of Chemistry & Biology of Membranes & Nanoobjects, CNRS UMR 5248, Université de Bordeaux, Bordeaux INP, 33600, Pessac, France. i.alves@cbmn.u-bordeaux.fr., Trifilieff P; Université de Bordeaux, INRAE, Bordeaux INP, NutriNeuro, 33000, Bordeaux, France. pierre.trifilieff@inrae.fr.

Publikováno v: Molecular psychiatry [Mol Psychiatry] 2023 May; Vol. 28 (5), pp. 2171.

Heterotrimeric G Protein Subunit Gαq Is a Master Switch for Gβγ-Mediated Calcium Mobilization by Gi-Coupled GPCRs.

Autor: Pfeil EM; Molecular, Cellular and Pharmacobiology Section, Institute for Pharmaceutical Biology, University of Bonn, Nussallee 6, 53115 Bonn, Germany; Research Training Group 1873, University of Bonn, Bonn, Germany., Brands J; Molecular, Cellular and Pharmacobiology Section, Institute for Pharmaceutical Biology, University of Bonn, Nussallee 6, 53115 Bonn, Germany; Research Training Group 1873, University of Bonn, Bonn, Germany., Merten N; Molecular, Cellular and Pharmacobiology Section, Institute for Pharmaceutical Biology, University of Bonn, Nussallee 6, 53115 Bonn, Germany., Vögtle T; Institute of Experimental Biomedicine I, University Hospital Würzburg and Rudolf Virchow Center, University of Würzburg, 97080 Würzburg, Germany., Vescovo M; Molecular, Cellular and Pharmacobiology Section, Institute for Pharmaceutical Biology, University of Bonn, Nussallee 6, 53115 Bonn, Germany., Rick U; Molecular, Cellular and Pharmacobiology Section, Institute for Pharmaceutical Biology, University of Bonn, Nussallee 6, 53115 Bonn, Germany., Albrecht IM; Molecular, Cellular and Pharmacobiology Section, Institute for Pharmaceutical Biology, University of Bonn, Nussallee 6, 53115 Bonn, Germany., Heycke N; Molecular, Cellular and Pharmacobiology Section, Institute for Pharmaceutical Biology, University of Bonn, Nussallee 6, 53115 Bonn, Germany., Kawakami K; Graduate School of Pharmaceutical Science, Tohoku University, Sendai 980-8578, Japan., Ono Y; Graduate School of Pharmaceutical Science, Tohoku University, Sendai 980-8578, Japan., Ngako Kadji FM; Graduate School of Pharmaceutical Science, Tohoku University, Sendai 980-8578, Japan., Hiratsuka S; Graduate School of Pharmaceutical Science, Tohoku University, Sendai 980-8578, Japan., Aoki J; Graduate School of Pharmaceutical Science, Tohoku University, Sendai 980-8578, Japan., Häberlein F; Molecular, Cellular and Pharmacobiology Section, Institute for Pharmaceutical Biology, University of Bonn, Nussallee 6, 53115 Bonn, Germany; Research Training Group 1873, University of Bonn, Bonn, Germany., Matthey M; Department of Systems Physiology, Medical Faculty, Ruhr University Bochum, 44801 Bochum, Germany; Institute of Physiology I, Life and Brain Center, Medical Faculty, University of Bonn, Venusberg-Campus 1, 53127 Bonn, Germany., Garg J; Institute of Pharmacology and Toxicology, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany., Hennen S; Molecular, Cellular and Pharmacobiology Section, Institute for Pharmaceutical Biology, University of Bonn, Nussallee 6, 53115 Bonn, Germany., Jobin ML; Institute of Pharmacology and Toxicology and Bio-Imaging Center, University of Würzburg, 97078 Würzburg, Germany., Seier K; Institute of Pharmacology and Toxicology and Bio-Imaging Center, University of Würzburg, 97078 Würzburg, Germany., Calebiro D; Institute of Pharmacology and Toxicology and Bio-Imaging Center, University of Würzburg, 97078 Würzburg, Germany; Institute of Metabolism and Systems Research and Centre of Membrane Proteins and Receptors, University of Birmingham, B15 2TT Birmingham, UK., Pfeifer A; Institute of Physiology I, Life and Brain Center, Medical Faculty, University of Bonn, Venusberg-Campus 1, 53127 Bonn, Germany., Heinemann A; Division of Pharmacology, Otto-Loewi Research Center for Vascular Biology, Immunology and Inflammation, Medical University of Graz, 8010 Graz, Austria., Wenzel D; Department of Systems Physiology, Medical Faculty, Ruhr University Bochum, 44801 Bochum, Germany; Institute of Physiology I, Life and Brain Center, Medical Faculty, University of Bonn, Venusberg-Campus 1, 53127 Bonn, Germany., König GM; Institute for Pharmaceutical Biology, University of Bonn, Nussallee 6, 53115 Bonn, Germany., Nieswandt B; Institute of Experimental Biomedicine I, University Hospital Würzburg and Rudolf Virchow Center, University of Würzburg, 97080 Würzburg, Germany., Fleischmann BK; Institute of Physiology I, Life and Brain Center, Medical Faculty, University of Bonn, Venusberg-Campus 1, 53127 Bonn, Germany., Inoue A; Graduate School of Pharmaceutical Science, Tohoku University, Sendai 980-8578, Japan., Simon K; Molecular, Cellular and Pharmacobiology Section, Institute for Pharmaceutical Biology, University of Bonn, Nussallee 6, 53115 Bonn, Germany. Electronic address: ksimon1@uni-bonn.de., Kostenis E; Molecular, Cellular and Pharmacobiology Section, Institute for Pharmaceutical Biology, University of Bonn, Nussallee 6, 53115 Bonn, Germany. Electronic address: kostenis@uni-bonn.de.

Publikováno v: Molecular cell [Mol Cell] 2020 Dec 17; Vol. 80 (6), pp. 940-954.e6. Date of Electronic Publication: 2020 Nov 16.

Selective and Wash-Resistant Fluorescent Dihydrocodeinone Derivatives Allow Single-Molecule Imaging of μ-Opioid Receptor Dimerization.

Autor: Gentzsch C; Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy and Food Chemistry, Julius Maximilian University of Würzburg, Am Hubland, 97074, Würzburg, Germany., Seier K; Institute of Pharmacology and Toxicology, Julius Maximilian University of Würzburg, Versbacher Strasse 9, 97078, Würzburg, Germany., Drakopoulos A; Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy and Food Chemistry, Julius Maximilian University of Würzburg, Am Hubland, 97074, Würzburg, Germany., Jobin ML; Institute of Pharmacology and Toxicology, Julius Maximilian University of Würzburg, Versbacher Strasse 9, 97078, Würzburg, Germany., Lanoiselée Y; Institute of Metabolism and Systems Research & Centre of Membrane Proteins and Receptors, University of Birmingham, IBR Tower, Level 2, Edgbaston, Birmingham, B152TT, UK., Koszegi Z; Institute of Metabolism and Systems Research & Centre of Membrane Proteins and Receptors, University of Birmingham, IBR Tower, Level 2, Edgbaston, Birmingham, B152TT, UK., Maurel D; ARPEGE (Pharmacology Screening Interactome) platform facility, Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, 141, rue de la Cardonille, 34094, Montpellier Cedex 05, France., Sounier R; Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, 141, rue de la Cardonille, 34094, Montpellier Cedex 05, France., Hübner H; Medicinal Chemistry, Department of Chemistry and Pharmacy, Friedrich-Alexander University of Erlangen-Nuremberg, 91058, Erlangen, Germany., Gmeiner P; Medicinal Chemistry, Department of Chemistry and Pharmacy, Friedrich-Alexander University of Erlangen-Nuremberg, 91058, Erlangen, Germany., Granier S; Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, 141, rue de la Cardonille, 34094, Montpellier Cedex 05, France., Calebiro D; Institute of Pharmacology and Toxicology, Julius Maximilian University of Würzburg, Versbacher Strasse 9, 97078, Würzburg, Germany.; Institute of Metabolism and Systems Research & Centre of Membrane Proteins and Receptors, University of Birmingham, IBR Tower, Level 2, Edgbaston, Birmingham, B152TT, UK., Decker M; Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy and Food Chemistry, Julius Maximilian University of Würzburg, Am Hubland, 97074, Würzburg, Germany.

Publikováno v: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2020 Apr 06; Vol. 59 (15), pp. 5958-5964. Date of Electronic Publication: 2020 Jan 07.

Hot spots for GPCR signaling: lessons from single-molecule microscopy.

Autor: Calebiro D; Institute of Metabolism and Systems Research, University of Birmingham, Birmingham, UK; Centre of Membrane Proteins and Receptors (COMPARE), Universities of Birmingham and Nottingham, UK; Institute of Pharmacology and Bio-Imaging Center, University of Würzburg, Germany. Electronic address: D.Calebiro@bham.ac.uk., Jobin ML; Institute of Pharmacology and Bio-Imaging Center, University of Würzburg, Germany.

Publikováno v: Current opinion in cell biology [Curr Opin Cell Biol] 2019 Apr; Vol. 57, pp. 57-63. Date of Electronic Publication: 2018 Dec 03.