Zobrazeno 1 - 10
of 31
pro vyhledávání: '"Joanne, Hothersall"'
Autor:
Anthony S Haines, Steve G Kendrew, Nicola Crowhurst, Elton R Stephens, Jack Connolly, Joanne Hothersall, Claire E Miller, Andrew J Collis, Benjamin D Huckle, Christopher M Thomas
Publikováno v:
PLoS ONE, Vol 17, Iss 5, p e0268072 (2022)
Pseudomonas strain NCIMB10586, in the P. fluorescens subgroup, produces the polyketide antibiotic mupirocin, and has potential as a host for industrial production of a range of valuable products. To underpin further studies on its genetics and physio
Externí odkaz:
https://doaj.org/article/36ac6982536c4cee99fcb464e59df433
Autor:
Stephen J. W. Busby, Joanne Hothersall, Rita E. Godfrey, Douglas F. Browning, Christos Fanitsios, Tim W. Overton
Publikováno v:
New Biotechnology. 64:1-8
Many commonly used bacterial promoters employed for recombinant protein production (RPP) in Escherichia coli are capable of high-level protein expression. However, such promoter systems are often too strong, being ill suited for expressing proteins t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0845b4f00c1c6ecd13328159f4e131eb
https://doi.org/10.1016/j.nbt.2021.05.001
https://doi.org/10.1016/j.nbt.2021.05.001
Autor:
Joanne Hothersall, Alexander Osgerby, Rita E. Godfrey, Tim W. Overton, Stephen J.W. Busby, Douglas F. Browning
Publikováno v:
New biotechnology. 72
We have developed a novel urea-inducible recombinant protein production system by exploiting the Proteus mirabilis urease ureR-ureD promoter region and the ureR AraC-family transcriptional regulator. Experiments using the expression of β-galactosida
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8687198d7835bd3edc998508edc9ec2e
https://pubmed.ncbi.nlm.nih.gov/36273806
https://pubmed.ncbi.nlm.nih.gov/36273806
Autor:
Joanne Hothersall, Sandie Lai, Nan Zhang, Rita E. Godfrey, Patcharawarin Ruanto, Sarah Bischoff, Colin Robinson, Tim W. Overton, Stephen J. W. Busby, Douglas F. Browning
Most Escherichia coli overexpression vectors used for recombinant protein production (RPP) depend on organic inducers, for example, sugars or simple conjugates. However, these can be expensive and, sometimes, chemically unstable. To simplify this and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ce1c4a09511085320934e777785d519
http://wrap.warwick.ac.uk/164107/1/WRAP-inexpensive-protein-overexpression-driven-NarL-transcription-activator-protein-2022.pdf
http://wrap.warwick.ac.uk/164107/1/WRAP-inexpensive-protein-overexpression-driven-NarL-transcription-activator-protein-2022.pdf
Autor:
Anthony S. Haines, Steve G. Kendrew, Nicola Crowhurst, Elton R. Stephens, Jack Connolly, Joanne Hothersall, Claire E. Miller, Andrew J. Collis, Benjamin D. Huckle, Christopher M. Thomas
Publikováno v:
PloS one. 17(5)
Pseudomonas strain NCIMB10586, in the P. fluorescens subgroup, produces the polyketide antibiotic mupirocin, and has potential as a host for industrial production of a range of valuable products. To underpin further studies on its genetics and physio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a239f8931987d67fa41ef9f8108dda18
https://pubmed.ncbi.nlm.nih.gov/35511780
https://pubmed.ncbi.nlm.nih.gov/35511780
Autor:
Stephen J. W. Busby, Rita E. Godfrey, David J. Lee, David L. Chismon, Joanne Hothersall, Douglas F. Browning, Patcharawarin Ruanto
Publikováno v:
Biochemical Journal
The Escherichia coli NarX/NarL two-component response-regulator system regulates gene expression in response to nitrate ions and the NarL protein is a global transcription factor, which activates transcript initiation at many target promoters. One su
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f6825f6caf2905d30105e4b6ff3dbf1
https://pubmed.ncbi.nlm.nih.gov/32662815
https://pubmed.ncbi.nlm.nih.gov/32662815
A natural plasmid uniquely encodes two biosynthetic pathways creating a potent anti-MRSA antibiotic.
Autor:
Daisuke Fukuda, Anthony S Haines, Zhongshu Song, Annabel C Murphy, Joanne Hothersall, Elton R Stephens, Rachel Gurney, Russell J Cox, John Crosby, Christine L Willis, Thomas J Simpson, Christopher M Thomas
Publikováno v:
PLoS ONE, Vol 6, Iss 3, p e18031 (2011)
Understanding how complex antibiotics are synthesised by their producer bacteria is essential for creation of new families of bioactive compounds. Thiomarinols, produced by marine bacteria belonging to the genus Pseudoalteromonas, are hybrids of two
Externí odkaz:
https://doaj.org/article/f65d8ef4119a44789cb398feeee148db
Autor:
Shu-Shan Gao, Luoyi Wang, Zhongshu Song, Joanne Hothersall, Elton R. Stevens, Jack Connolly, Peter J. Winn, Russell J. Cox, Matthew P. Crump, Paul R. Race, Christopher M. Thomas, Thomas J. Simpson, Christine L. Willis
Publikováno v:
Angewandte Chemie. 129:3988-3992
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::27b63e99e595f28a702cd79dda8067e7
https://doi.org/10.1002/ange.201611590
https://doi.org/10.1002/ange.201611590
Autor:
Jack A, Connolly, Amber, Wilson, Malgorzata, Macioszek, Zhongshu, Song, Luoyi, Wang, Hadi H, Mohammad, Mukul, Yadav, Maura, di Martino, Claire E, Miller, Joanne, Hothersall, Anthony S, Haines, Elton R, Stephens, Matthew P, Crump, Christine L, Willis, Thomas J, Simpson, Peter J, Winn, Christopher M, Thomas
Publikováno v:
Scientific Reports
The mupirocin trans-AT polyketide synthase pathway, provides a model system for manipulation of antibiotic biosynthesis. Its final phase involves removal of the tertiary hydroxyl group from pseudomonic acid B, PA-B, producing the fully active PA-A in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::322599a316d1a6c01eee44f709a7fa47
https://pubmed.ncbi.nlm.nih.gov/30733464
https://pubmed.ncbi.nlm.nih.gov/30733464
Autor:
Joanne Hothersall, Zhongshu Song, Jack A. Connolly, Thomas J. Simpson, Peter J. Winn, Hadi H. Mohammad, Paul R. Race, Christine L. Willis, Christopher M. Thomas
Publikováno v:
Mohammad, H H, Connolly, J A, Song, Z, Hothersall, J, Race, P R, Willis, C L, Simpson, T J, Winn, P J & Thomas, C M 2018, ' Fine Tuning of Antibiotic Activity by a Tailoring Hydroxylase in a Trans-AT Polyketide Synthase Pathway ', ChemBioChem, vol. 19, no. 8, pp. 836-841 . https://doi.org/10.1002/cbic.201800036
The addition or removal of hydroxy groups modulates the activity of many pharmacologically active biomolecules. It can be integral to the basic biosynthetic factory or result from associated tailoring steps. For the anti-MRSA antibiotic mupirocin, re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15d2026ceac28f9acda4ee2da1cf8ce8
http://www.scopus.com/inward/record.url?scp=85043569007&partnerID=8YFLogxK
http://www.scopus.com/inward/record.url?scp=85043569007&partnerID=8YFLogxK